Accumulation regularity of isoflavone and its components during soybean seed development

doi:10.16178/j.issn.0528-9017.20240464

Abstract

Abstract:

Isoflavones, also known as phytoestrogens, are a class of secondary metabolites mostly produced in soybean seeds. This study focused on the differences about total isoflavone content and 12 isoflavone components during seed development among five soybean varieties using high performance liquid chromatography. During the progressing of seed development, the content of isoflavones continued to increase in the seeds of the five soybean varieties. However, there were significant differences in the accumulation mode and rate among the varieties. The proportion of the main components such as malonyl genistein, malonyl daidzein, genistein and daidzein changed among the five varieties according to the total isoflavone content. The accumulation pattern of genistein and daidzein (two physiological active components) were also different compared with the main components. The breeding and production of high-quality soybean varieties with high isoflavone content is supported by studying the physiological mechanism of isoflavone accumulation in soybean seeds.

Key words: soybean, isoflavone, seed development, accumulation regularity

CLC Number:

S565.1

YU Xiaomin, YUAN Fengjie, FU Xujun, YANG Qinghua, JIN Hangxia, ZHU Longming. Accumulation regularity of isoflavone and its components during soybean seed development[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(4): 904-909.

Figures/Tables 8

References 21

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品种	染料木苷类					黄豆苷类					黄豆黄素苷类						总含量
品种	G	MG	AG	Ge	合计	D	MD	AD	De	合计	GL	MGL	AGL	Gle	合计		总含量
中豆27	483.75	1 379.45	33.48	36.96	1 933.64	432.84	1 253.64	38.99	28.53	1 754.00	82.89	120.97	139.86	12.86	356.58	4 044.22
浙秋豆2号	361.36	822.97	26.19	33.20	1 243.72	128.72	279.09	10.61	11.96	430.38	55.26	70.44	86.69	29.02	241.41	1 915.51
浙春豆2014	389.56	967.52	37.97	56.51	1 451.56	218.12	574.11	25.00	37.51	854.74	56.35	51.84	100.25	22.42	230.86	2 537.16
九月黄	202.51	720.86	21.68	18.92	963.97	99.62	320.37	13.57	12.38	445.94	32.24	45.88	69.14	7.84	155.10	1 565.01
浙夏豆2022	238.53	622.19	20.44	20.63	901.79	94.56	227.08	9.76	12.33	343.73	55.41	72.99	65.08	22.36	215.84	1 461.36
平均	335.14	902.60	27.95	33.24	1 298.94	194.77	530.86	19.59	20.54	765.76	56.43	72.42	92.20	18.90	239.95	2 304.65

品种	染料木苷类					黄豆苷类					黄豆黄素苷类						总含量
品种	G	MG	AG	Ge	合计	D	MD	AD	De	合计	GL	MGL	AGL	Gle	合计		总含量
中豆27	483.75	1 379.45	33.48	36.96	1 933.64	432.84	1 253.64	38.99	28.53	1 754.00	82.89	120.97	139.86	12.86	356.58	4 044.22
浙秋豆2号	361.36	822.97	26.19	33.20	1 243.72	128.72	279.09	10.61	11.96	430.38	55.26	70.44	86.69	29.02	241.41	1 915.51
浙春豆2014	389.56	967.52	37.97	56.51	1 451.56	218.12	574.11	25.00	37.51	854.74	56.35	51.84	100.25	22.42	230.86	2 537.16
九月黄	202.51	720.86	21.68	18.92	963.97	99.62	320.37	13.57	12.38	445.94	32.24	45.88	69.14	7.84	155.10	1 565.01
浙夏豆2022	238.53	622.19	20.44	20.63	901.79	94.56	227.08	9.76	12.33	343.73	55.41	72.99	65.08	22.36	215.84	1 461.36
平均	335.14	902.60	27.95	33.24	1 298.94	194.77	530.86	19.59	20.54	765.76	56.43	72.42	92.20	18.90	239.95	2 304.65

品种	R5	R6	R7	R8
中豆27	274.40 b	771.55 a	2 444.19 a	4 044.22 a
浙秋豆2号	367.87 a	522.18 c	898.66 c	1 915.51 c
浙春豆2014	178.78 d	600.03 b	1 536.42 b	2 537.16 b
九月黄	295.12 b	525.93 c	1 419.72 b	1 565.01 d
浙夏豆2022	219.44 c	286.95 d	583.61 d	1 461.36 d
平均	267.12	541.33	1 376.52	2 304.65

品种	R5	R6	R7	R8
中豆27	274.40 b	771.55 a	2 444.19 a	4 044.22 a
浙秋豆2号	367.87 a	522.18 c	898.66 c	1 915.51 c
浙春豆2014	178.78 d	600.03 b	1 536.42 b	2 537.16 b
九月黄	295.12 b	525.93 c	1 419.72 b	1 565.01 d
浙夏豆2022	219.44 c	286.95 d	583.61 d	1 461.36 d
平均	267.12	541.33	1 376.52	2 304.65

品种	R5		R6		R7		R8
品种	MG含量/ (μg·g^-1)	占比/ %	MG含量/ (μg·g^-1)	占比/ %	MG含量/ (μg·g^-1)	占比/ %	MG含量/ (μg·g^-1)	占比/ %
中豆27	40.81 c	14.87	211.25 a	27.38	751.79 a	30.76	1 379.45 a	34.11
浙秋豆2号	102.92 a	27.98	164.55 c	31.51	329.62 c	36.68	822.97 c	42.96
浙春豆2014	41.68 c	23.32	180.29 b	30.05	590.33 b	38.42	967.52 b	38.13
九月黄	49.30 b	16.70	175.02 b	33.28	609.02 b	42.90	720.86 d	46.06
浙夏豆2022	37.40 d	17.04	70.73 d	24.65	183.52 d	31.44	622.19 e	42.58