Principal component analysis and comprehensive evaluation of cold and hypoxia resistance of different tobacco varieties at the seedling stage

doi:10.16178/j.issn.0528-9017.20240496

Abstract

Abstract:

Using 15 tobacco varieties as experimental materials, two treatments were applied to the tobacco seedlings: distilled water (control) and a combination of 5 ℃ low temperature with 1.28 mg·L^-1 dissolved oxygen concentration (cold and hypoxia stress, CH). Seedling height, fresh weight, dry weight, chlorophyll relative content, seedling fresh weight, and root fresh weight under different treatments were measured for each tobacco variety. Statistical analyses, including difference significance analysis, correlation analysis, principal component analysis (PCA) and cluster analysis, were used to evaluate cold and hypoxia resistance and to screen the tobacco varieties. Compared with the control, all measured indicators of the tested tobacco varieties were affected to varying degrees under the cold and hypoxia stress. There was a significant or extremely significant correlation between cold and hypoxia resistance coefficients of most test indicators. Using principal component analysis, cold and hypoxia resistance coefficients of six individual indicators were divided into two principal components, with a cumulative variance contribution rate of 75.003%. Based on cluster analysis of the comprehensive scores, the 15 tobacco varieties were classified into 4 categories: one CH-tolerant variety: MS Yunyan87; eight moderately tolerant varieties: Yunyan 121, NC82, Yunyan 97, Yunyan 100, NC55, NC297, Honghua dajinyuan and Yunyan 116; two general sensitive varieties: K326 and KRK26; four sensitive varieties: Yunxue 2, Yunxue 1, V2 and G28.

Key words: tobacco(Nicotiana tabacum L.), seedling stage, cold and hypoxia stress, variety screening

CLC Number:

S572

SUO Wenlong, LI Longxi, NIU Yongzhi, GUAN Yajing, ZHENG Yunye. Principal component analysis and comprehensive evaluation of cold and hypoxia resistance of different tobacco varieties at the seedling stage[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(6): 1355-1362.

Figures/Tables 7

References 52

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处理	溶解氧浓度/ (mg·L^-1)	溶解氧含量/ %
200 mL营养液+0 g无水亚硫酸钠	8.50	100.00
200 mL营养液+0.01 g无水亚硫酸钠	8.22	92.00
200 mL营养液+0.02 g无水亚硫酸钠	5.54	61.60
200 mL营养液+0.03 g无水亚硫酸钠	4.16	46.40
200 mL营养液+0.04 g无水亚硫酸钠	1.28	14.30

处理	溶解氧浓度/ (mg·L^-1)	溶解氧含量/ %
200 mL营养液+0 g无水亚硫酸钠	8.50	100.00
200 mL营养液+0.01 g无水亚硫酸钠	8.22	92.00
200 mL营养液+0.02 g无水亚硫酸钠	5.54	61.60
200 mL营养液+0.03 g无水亚硫酸钠	4.16	46.40
200 mL营养液+0.04 g无水亚硫酸钠	1.28	14.30

品种编号	抗低温低氧系数(CHC)
品种编号	苗高	鲜重	干重	叶绿素相对含量	苗鲜重	根鲜重
1	1.04±0.03 bcd	1.18±0.05 bcd	1.33±0.13 cd	0.84±0.03 de	1.44±0.38 def	0.84±0.07 ef
2	1.02±0.04 bcde	1.19±0.05 bcd	1.43±0.08 bc	0.87±0 c	1.35±0.12 efg	1.09±0.09 b
3	0.92±0.06 cde	0.87±0.09 fg	1.07±0.07 d e	0.82±0 e	0.95±0.02 hi	0.92±0.04 de
4	1.02±0.10 bcd	0.91±0.14 efg	1.10±0.14 de	0.81±0.02 e	1.08±0.07 ghi	0.71±0.07 gh
5	0.86±0.07 e	1.12±0.09 bcdef	1.31±0.12 cd	0.87±0.03 c	0.82±0.04 i	0.49±0 j
6	1.15±0.10 ab	1.37±0.22 abc	1.73±0.22 a	1.02±0.02 a	1.19±0.08 efgh	0.76±0.01 fg
7	0.89±0.04 de	0.77±0.14 g	0.95±0.20 e	0.76±0 f	1.10±0.05 ghi	0.57±0.03 ij
8	1.07±0.10 bc	1.29±0.20 abcd	1.60±0.17 ab	0.87±0.04 c	1.16±0.05 fgh	1.23±0.08 a
9	0.99±0.07 cde	0.90±0.02 efg	1.14±0.03 de	0.76±0.01 f	1.70±0.10 cd	1.01±0.10 bcd
10	0.88±0.07 de	1.10±0.12 cdef	1.32±0.07 cd	1.00±0.02 a	2.46±0.24 a	1.12±0.05 b
11	0.97±0.04 cde	1.10±0.25 cdef	1.24±0.16 cd	0.93±0.01 b	2.10±0.12 b	0.95±0.09 cde
12	1.03±0.10 bcd	1.15±0.07 bcde	1.33±0.06 cd	0.93±0.03 b	1.49±0.40 de	1.11±0.05 b
13	1.01±0.11 bcde	1.39±0.11 ab	1.74±0.17 a	0.86±0.02 cd	1.81±0.15 bc	0.62±0.03 hi
14	1.05±0.07 bc	1.02±0.14 defg	1.10±0.13 de	1.00±0 a	2.07±0.05 b	1.06±0.10 bc
15	1.28±0.13 a	1.54±0.21 a	1.69±0.21 a	0.93±0.01 b	1.84±0.08 bc	0.79±0.08 fg

品种编号	抗低温低氧系数(CHC)
品种编号	苗高	鲜重	干重	叶绿素相对含量	苗鲜重	根鲜重
1	1.04±0.03 bcd	1.18±0.05 bcd	1.33±0.13 cd	0.84±0.03 de	1.44±0.38 def	0.84±0.07 ef
2	1.02±0.04 bcde	1.19±0.05 bcd	1.43±0.08 bc	0.87±0 c	1.35±0.12 efg	1.09±0.09 b
3	0.92±0.06 cde	0.87±0.09 fg	1.07±0.07 d e	0.82±0 e	0.95±0.02 hi	0.92±0.04 de
4	1.02±0.10 bcd	0.91±0.14 efg	1.10±0.14 de	0.81±0.02 e	1.08±0.07 ghi	0.71±0.07 gh
5	0.86±0.07 e	1.12±0.09 bcdef	1.31±0.12 cd	0.87±0.03 c	0.82±0.04 i	0.49±0 j
6	1.15±0.10 ab	1.37±0.22 abc	1.73±0.22 a	1.02±0.02 a	1.19±0.08 efgh	0.76±0.01 fg
7	0.89±0.04 de	0.77±0.14 g	0.95±0.20 e	0.76±0 f	1.10±0.05 ghi	0.57±0.03 ij
8	1.07±0.10 bc	1.29±0.20 abcd	1.60±0.17 ab	0.87±0.04 c	1.16±0.05 fgh	1.23±0.08 a
9	0.99±0.07 cde	0.90±0.02 efg	1.14±0.03 de	0.76±0.01 f	1.70±0.10 cd	1.01±0.10 bcd
10	0.88±0.07 de	1.10±0.12 cdef	1.32±0.07 cd	1.00±0.02 a	2.46±0.24 a	1.12±0.05 b
11	0.97±0.04 cde	1.10±0.25 cdef	1.24±0.16 cd	0.93±0.01 b	2.10±0.12 b	0.95±0.09 cde
12	1.03±0.10 bcd	1.15±0.07 bcde	1.33±0.06 cd	0.93±0.03 b	1.49±0.40 de	1.11±0.05 b
13	1.01±0.11 bcde	1.39±0.11 ab	1.74±0.17 a	0.86±0.02 cd	1.81±0.15 bc	0.62±0.03 hi
14	1.05±0.07 bc	1.02±0.14 defg	1.10±0.13 de	1.00±0 a	2.07±0.05 b	1.06±0.10 bc
15	1.28±0.13 a	1.54±0.21 a	1.69±0.21 a	0.93±0.01 b	1.84±0.08 bc	0.79±0.08 fg

指标	x₁	x₂	x₃	x₄	x₅
x₂	0.709^{* *}
x₃	0.629^*	0.956^{* *}
x₄	0.357	0.525^*	0.428
x₅	0.123	0.219	0.114	0.510
x₆	0.138	0.040	0.013	0.312	0.409