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    11 June 2025, Volume 66 Issue 6 Previous Issue   

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    Progress in molecular mechanism research of indica-japonica hybrid rice
    MA Shuangshuang, HUANG Lingling, WANG Wei, YANG Yongdan, ZHAI Rongrong, YE Jing, WU Mingming, YE Shenghai
    2025, 66 (6):  1297-1307.  DOI: 10.16178/j.issn.0528-9017.20240808
    Abstract ( 26 )   HTML ( 6 )   PDF (1833KB) ( 32 )  

    Asian cultivated rice (Oryza sativa L.) has differentiated into two subspecies, indica and japonica, during domestication, exhibiting significant variations in cultivation environments, plant and grain characteristics, and genome. The effective utilization of genetic divergence between indica and japonica subspecies could further enhance rice yield through their strong heterosis. This paper systematically reviews the taxonomic classification and differentiation characteristics between indica and japonica, discusses currently mapped and cloned intersubspecific hybrid sterility genes and their molecular mechanisms, analyzes identified heterosis-related genes and their functional mechanisms, and compiles data of certified indica-japonica hybrid rice varieties approved at provincial or national levels. These comprehensive analyses aim to provide valuable insights for advancing research on intersubspecific heterosis utilization and developing novel indica-japonica hybrid rice cultivars.

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    Effects of different varieties and transplanting densities on salt tolerance, root system, and yield of rice in coastal saline-alkali land
    GAN Yingying, QIU Gaoyang, GUO Bin, FU Qinglin, ZHANG Junbo, LIU Junli, CHEN Xiaodong
    2025, 66 (6):  1308-1314.  DOI: 10.16178/j.issn.0528-9017.20250034
    Abstract ( 29 )   HTML ( 3 )   PDF (1971KB) ( 26 )  

    The effects of 3 rice varieties(Yueyou 938, Zhongyou 208 and Xieyou 205) and transplanting density on salt tolerance, root distribution and yield of rice were studied in Hangzhou Bay coastal saline-alkali land. The results showed that after treatment with 6 g·L-1 NaCl solution, the salt tolerance index of root of Yueyou 938, Zhongyou 208 and Xieyou 205 was greater than 70%, and the salt tolerance index of bud was greater than 80%. After treatment with 10 g·L-1 NaCl solution, only the salt tolerance index of root of Xieyou 205 was greater than 60%, while the salt tolerance index of buds was greater than 70%, so Xieyou 205 was identified as a salt-tolerant variety. The root biomass, root length density and rice yield were the highest with the basic seedling number of 450 000 per hm2 when the basic seedling number was 150 000, 300 000, 450 000 and 600 000 seedlings per hm2. Therefore, Xieyou 205 is a suitable rice variety for planting in Hangzhou Bay coastal saline-alkali land, and the basic number of seedlings suitable for planting is 450 000 per hm2.

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    Effects of rapeseed-rice-bean rotation on soil physicochemical properties and rice trace element content
    XU Youxiang, ZHU Zhenling, WANG Yufei, JIANG Jianfeng, YANG Haijun, DONG Xiangwei, LI Chengyong, CHEN Jinpeng, XU Kan, YE Yihao, ZHANG Yan, WANG Honghang, SHAO Guosheng
    2025, 66 (6):  1315-1321.  DOI: 10.16178/j.issn.0528-9017.20240757
    Abstract ( 28 )   HTML ( 2 )   PDF (1927KB) ( 29 )  

    To investigate the effects of the new triple-cropping rotation model of rapeseed-rice-bean on soil physicochemical properties and rice trace element content, and to promote the improvement and standardization of cultivation techniques for this rotation model, a field positioning experiment was conducted. Field positioning experiment was conducted with two treatments: continuous early rice-late rice cropping and rapeseed-rice-bean rotation, analyzing the effects of different treatments on basic soil physicochemical properties and rice trace element content. The results showed that, compared with traditional continuous cropping, the soil pH value significantly decreased after two years of rapeseed-rice-bean rotation, alkali-hydrolyzable nitrogen and available potassium content increased significantly,but no significant difference was observed in cation exchange capacity, bulk density and organic matter, total nitrogen, total phosphorus, total potassium and available phosphorus content. After two years of rapeseed-rice-bean rotation, the soil alkali-hydrolyzable nitrogen and available potassium content significantly increased, and available phosphorus content increased, but the difference was not significant. After two years of rapeseed-rice-bean rotation, the total content of soil iron, manganese, zinc, copper, and cadmium showed no significant difference, but the available content significantly increased. After two years of rapeseed-rice-bean rotation, the cadmium content in the above-ground straw and brown rice of early rice significantly increased. Under the conditions of this experiment, rapeseed-rice-bean rotation can effectively improve the level of soil available nutrients, but it also significantly reduces soil pH value content, and poses a risk of heavy metal cadmium pollution in early rice.

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    Effects of different forms of nitrogen nutrition on the growth of rice seedlings under tetracycline stress
    CHEN Min, SUN-LUO Mingsheng, SHEN Jiaming, YE Youxiang, ZHAO Guohua
    2025, 66 (6):  1322-1327.  DOI: 10.16178/j.issn.0528-9017.20230631
    Abstract ( 26 )   HTML ( 4 )   PDF (2930KB) ( 29 )  

    To investigate the effects of different forms of nitrogen nutrition on the growth of rice seedlings under tetracycline antibiotic stress, a nutrient solution culture method was employed. Under different nitrogen forms ( NH 4 +-N and NO 3 --N), various levels of tetracycline stress were applied to study the changes in dry matter accumulation, root-shoot ratio, leaf area, chlorophyll content, and antioxidant enzyme activities in rice. The results showed that different forms of nitrogen nutrition could regulate the impact of tetracycline stress on rice growth. Under low-dose tetracycline stress, NH 4 +-N was beneficial for promoting rice growth and increasing dry weight in both aboveground and underground parts. In contrast, under NO 3 --N treatment, the dry weight in both aboveground and underground parts was not significantly affected by tetracycline content. As the tetracycline content increased, the content of chlorophyll a and b decreased under NO 3 --N treatment, while the total nitrogen content in both aboveground and underground parts increased. Under NH 4 +-N treatment, the content of chlorophyll a and b and plant nitrogen accumulation did not show significant differences, with the highest total nitrogen content in both aboveground and underground parts observed under low-content tetracycline. As the tetracycline content increased, the POD activity in rice increased under different forms of nitrogen nutrition, while SOD activity showed no significant change under NH 4 +-N treatment and even decreased under NO 3 --N treatment. These findings suggest that the stress effects of tetracycline on rice plants can be regulated through different forms of nitrogen nutrition.

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    Effects of optimizing fertilization on the yield and nutrient utilization efficiency of Yongyou 1540
    WANG Xuefeng, MA Liya, HU Zefan, HUANG Yimian, LI Xiao, YE Tingyun, YE Xuezhu, CHEN De
    2025, 66 (6):  1328-1332.  DOI: 10.16178/j.issn.0528-9017.20250231
    Abstract ( 21 )   HTML ( 3 )   PDF (1577KB) ( 25 )  

    The irrational application of chemical fertilizers is one of the major issues in China's agricultural environment. Exploring region-specific fertilizer reduction technologies, determining appropriate reduction ratios, and improving fertilizer use efficiency are of significant importance. In this study, the late rice cultivar Yongyou 1540 was used as experimental material. A field experiment was conducted with five treatments: no fertilization (CK), no nitrogen (N0), no phosphorus (P0), no potassium (K0), and optimized fertilization (NPK). The effects of different treatments on rice yield, nutrient uptake, and fertilizer utilization efficiency were investigated.The results showed that there was no significant difference in rice yield between CK and N0 treatments. Similarly, no significant differences were observed among P0, K0, and NPK treatments, but their yields were significantly higher than those of CK and N0. Compared with CK, the P0, K0, and NPK treatments increased rice yield by 25.1%, 22.2%, and 24.7%, respectively; straw biomass increased by 27.9%, 31.4%, and 25.0%, respectively; grain nitrogen content increased by 35.8%, 33.7%, and 37.4%, respectively; and straw nitrogen content increased by 102.4%, 60.7%, and 79.3%, respectively. The nitrogen, phosphorus, and potassium fertilizer use efficiencies were 44.9%, 8.9%, and 25.2%, respectively. This study demonstrates that, under the current experimental conditions, nitrogen is the decisive factor affecting rice yield, whereas phosphorus and potassium exhibit no significant yield-increasing effects. Future fertilization strategies should prioritize ensuring nitrogen supply while reducing phosphorus and potassium application rates.

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    Effects of different fertilization dose before and after topping on yield and quality of rapeseed
    WANG Nuan, SHAO Ting, WU Meijuan, HUANG Hongming, ZHANG Yanan
    2025, 66 (6):  1333-1337.  DOI: 10.16178/j.issn.0528-9017.20240772
    Abstract ( 16 )   HTML ( 2 )   PDF (1143KB) ( 29 )  

    To study the effects of different fertilization dose before and after topping on the yield and quality of rapeseed, a related experiment was conducted in Lanxi City from 2023 to 2024. The rapeseed shoots were processed into dried pickled vegetable, and their nutritional components were analyzed. The results showed that topping delayed the growth period of rapeseed, reduced plant height, and increased the protein content of rapeseed. As the fertilization dose increased before and after topping, the growth period was correspondingly delayed, the number of primary branches slightly increased, the number of pods per plant showed no clear trend, and the overall rapeseed yield tended to increase. In summary, when urea application was ≥7.5 kg per 667 m2 either on 5 days before topping or on the day of topping, the rapeseed yield was higher, and the comprehensive benefits of the rapeseed field were improved. The dried pickled vegetable made from rapeseed shoots have high nutritional value and are worth further promotion.

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    Study on harvesting methods of rapeseed (Brassica napus L.) shoots for vegetable and green manure dual-use in winter fallow fields
    WU Zhaohui, WANG Mengmeng, DONG Ningyu, SONG Yandan, WANG Dian, LIU Qiaozhen, XU Wenzheng, HAN Huige, CHEN Guangqing
    2025, 66 (6):  1338-1343.  DOI: 10.16178/j.issn.0528-9017.20250175
    Abstract ( 16 )   HTML ( 3 )   PDF (1538KB) ( 26 )  

    Utilizing fallow winter farmland to plant rapeseed for vegetable and green manure dual-use can increase the income of tobacco farmers, improve soil quality, balance nutrients, prevent soil-borne diseases, and enhance the yield and quality of subsequent crops. A field experiment was conducted using the Fengyou 10 variety as the test material to study the effects of harvesting rapeseed shoots at different heights on the yield (weight fresh) and quality of bolt, as well as the biomass before overturning of rapeseed. The results showed that the highest yield of rapeseed bolt was achieved when harvested at a height of 20 cm, while the lowest yield was observed when harvested at a height of 30 cm. The trend in biomass before overturning was the opposite. Harvesting methods on the quality of rapeseed stems varied depending on the growth height and cutting length at the time of harvesting. When the stems were harvested at a height of 20 cm, the soluble sugar content is the highest if they were cut from the base. At a height of 30 cm, the stems exhibited significantly higher levels of crude fiber, protein, and Zn compared with other treatments. When harvested at 40 cm, the vitamin C content was significantly higher than in other treatments. Harvesting at 20 cm resulted in a significantly lower biomass before overturning compared with other treatments. No significant differences were observed among the other treatments. For winter fallow field planted with rapeseed for vegetable and green manure dual-use, shoots harvested at a height of 40 cm with a 25 cm topping length. This approach ensures high rapeseed shoots yield while maintaining sufficient biomass for green manure, both economic and ecological benefits.

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    Effects of green waste composting on growth, quality and soil properties of Chinese rape
    XU Yiyao, LI Siqi, DONG Haixian, LIU Qiang, LIU Shuxin
    2025, 66 (6):  1344-1348.  DOI: 10.16178/j.issn
    Abstract ( 17 )   HTML ( 3 )   PDF (3285KB) ( 23 )  

    To clarify the effect of applying green waste composting in vegetable planting, Chinese rape were used as experimental materials to explore the effects of dosages of green waste composting on the growth, quality indicators, and soil properties of Chinese rape. The results showed that the application of green waste composting to the soil significantly promoted the growth of Chinese rape. The plant height, stem diameter, leaf number and fresh weight increased significantly. The contents of vitamin C, soluble protein and soluble sugar were significantly increased, and the content of nitrite was significantly reduced. The soil pH value increased significantly, and the contents of soil organic matter, alkali hydrolyzable nitrogen, available phosphorus and available potassium increased significantly. Different dosages of green waste composting showed different effects on the growth,quality,and soil properties of Chinese rape. The contents of vitamin C, soluble protein and soluble sugar showed a trend of first increasing and then decreasing, while the nitrite content showed a trend of first decreasing and then increasing with the increase of the application dosages of green waste composting. The treatment effect of 40% addition of green waste composting was the best. Soil nutrient contents increased with the increase of the application dosages of green waste composting.Based on a comprehensive analysis of the growth, quality, and soil properties of Chinese rape, it is recommended to apply 40% green waste composting.

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    Relationship between soil acidity and exchangeable hydrogen and aluminum in tea gardens of Guangxi
    YIN Beibei, HUANG Jinsheng, WANG Lei, ZHOU Liuqiang, ZHU Xiaohui, PENG Jiayu, XIE Rulin, ZENG Yan, OU Huiping, LI Zhongning, LU Hongmei, MO Wenbin, HE Dongqiu, LIU Lijun, ZHANG Xian
    2025, 66 (6):  1349-1354.  DOI: 10.16178/j.issn.0528-9017.20240200
    Abstract ( 13 )   HTML ( 2 )   PDF (3547KB) ( 25 )  

    To study the relationship between soil acidity and exchangeable hydrogen and aluminium in the main tea gardens of Guangxi, and to provide technical support for the scientific cultivation of tea trees and the improvement of acidified soil, 290 soil samples were collected from the tea tree planting areas in Guangxi, and their pH values, exchangeable hydrogen and aluminum contents were analyzed. The results show that the pH value of the soil in the tea-growing areas of Guangxi fluctuates greatly, ranging from 3.11 to 6.89. 66.55% of the soil samples have a pH value lower than 4.5, and the soil in the tea gardens is extremely acidic. The smaller the pH value, the greater the content of exchangeable acids. Exchangeable acid is the main factor affecting soil pH value. When the pH value is low, the content of exchangeable H+ and exchangeable Al3+ will be higher. There is a significant linear positive correlation between soil exchangeable acid and exchangeable H+ and exchangeable Al3+ With the increase of the contents of H+ and Al3+, soil exchangeable acid will also increase accordingly. The contribution of exchangeable Al3+ to soil acidity is greater than that of exchangeable H+ to soil acidity. It can be seen that the soil in tea gardens in Guangxi is severely acidified. In production, soil acidity improvement should be carried out in a timely manner.

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    Principal component analysis and comprehensive evaluation of cold and hypoxia resistance of different tobacco varieties at the seedling stage
    SUO Wenlong, LI Longxi, NIU Yongzhi, GUAN Yajing, ZHENG Yunye
    2025, 66 (6):  1355-1362.  DOI: 10.16178/j.issn.0528-9017.20240496
    Abstract ( 13 )   HTML ( 2 )   PDF (1679KB) ( 25 )  

    Using 15 tobacco varieties as experimental materials, two treatments were applied to the tobacco seedlings: distilled water (control) and a combination of 5 ℃ low temperature with 1.28 mg·L-1 dissolved oxygen concentration (cold and hypoxia stress, CH). Seedling height, fresh weight, dry weight, chlorophyll relative content, seedling fresh weight, and root fresh weight under different treatments were measured for each tobacco variety. Statistical analyses, including difference significance analysis, correlation analysis, principal component analysis (PCA) and cluster analysis, were used to evaluate cold and hypoxia resistance and to screen the tobacco varieties. Compared with the control, all measured indicators of the tested tobacco varieties were affected to varying degrees under the cold and hypoxia stress. There was a significant or extremely significant correlation between cold and hypoxia resistance coefficients of most test indicators. Using principal component analysis, cold and hypoxia resistance coefficients of six individual indicators were divided into two principal components, with a cumulative variance contribution rate of 75.003%. Based on cluster analysis of the comprehensive scores, the 15 tobacco varieties were classified into 4 categories: one CH-tolerant variety: MS Yunyan87; eight moderately tolerant varieties: Yunyan 121, NC82, Yunyan 97, Yunyan 100, NC55, NC297, Honghua dajinyuan and Yunyan 116; two general sensitive varieties: K326 and KRK26; four sensitive varieties: Yunxue 2, Yunxue 1, V2 and G28.

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    Spatiotemporal variation characteristics and suitability evaluation of soil nutrients in tobacco-growing areas of southwestern Hubei
    CHEN Chengli, WEN Tao, ZHANG Fang, XIONG Tao, QIN Yongsheng, WANG Junyong, ZHOU Yuzi, LUO Fang, MA Fanglei, RAN Juan
    2025, 66 (6):  1363-1368.  DOI: 10.16178/j.issn.0528-9017.20250091
    Abstract ( 13 )   HTML ( 3 )   PDF (1202KB) ( 27 )  

    To clarify the spatiotemporal variation characteristics of soil nutrients in tobacco-growing soils in the mountainous areas of southwestern Hubei and optimize tobacco-growing layouts and balanced fertilization practices, 578 tobacco-growing soil samples from 10 townships in Jianshi County were collected and analyzed in 2018 and 2021. The results indicated that, compared with 2018, the soil pH value, organic matter, and total nitrogen content in 2021 decreased by 0.55, 6.06 g·kg-1, and 0.47 g·kg-1, respectively, while the available phosphorus and available potassium content increased by 9.91 and 37.62 mg·kg-1, respectively. The average integrated fertility index in 2021 was 0.72, showing an increase compared with 2018. Based on these findings, measures such as increasing organic fertilizer application and adjusting potassium fertilization methods are recommended to improve soil fertility, enhance potassium utilization efficiency, and increase organic matter and total nitrogen content in Jianshi County's tobacco-growing soils.

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    Effects of shading time on the growth and photosynthetic physiological characteristics of cigar wrapper leaves
    SONG Hao, ZHANG Wenhan, WANG Yanyan, QIAO Baoming, DU Fu, ZHAO Zeyu, FAN Wenpeng, WANG Haoli, SHAN Yujing, TIAN Lijun, LI Ziwei, LIU Liping, YANG Yongxia
    2025, 66 (6):  1369-1376.  DOI: 10.16178/j.issn.0528-9017.20250172
    Abstract ( 15 )   HTML ( 2 )   PDF (7086KB) ( 28 )  

    This study aims to clarify the impact of shading time on the growth and development, photosynthetic physiological characteristics of and cigar wrapper leaves and physical characteristics of cured cigar leaves. Using the cigar variety Chuxue 80 (CX-80) as the material, four treatments were established: no shading (T1), shading throughout the whole growth period (T2), shading from the 20 days after transplanting (T3), shading from the 40 days after transplanting (T4). The effects of shading time on the plant height, leaf morphology, photosynthetic characteristics, physical properties of cured leaves and vein thickness of CX-80 were analyzed. The results showed that shading throughout the whole growth period (T2) significantly increased the plant height and maximum leaf length but reduced the stem circumference. Shading before the twentieth days after transplanting significantly reduced the net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr), as well as lower relative leaf temperature and thinner leaves. Chlorophyll fluorescence parameter analysis showed that, with the increase of shading time, the initial fluorescence (Fo) increases, the maximum fluorescence (Fm), the actual photochemical efficiency Y(Ⅱ), photochemical quenching coefficient (qP) and non-photochemical quenching coefficient (qN) gradually decreased. Leaf environmental scanning electron microscope results showed that, with the increase of shading time, the number of leaf stomata decreased and the stomata area and circumference gradually increased. After curing and fermentation, the stalk rate, leaf vein diameter and main and branch vein diameter gradually decreased. In conclusion, shading time affects the growth and development of cigar leaves, photosynthetic physiological characteristics and the physical characteristics of cured tobacco leaves, with potential to modify the growing environment and improve the availability of cigar wrapper leaves through different shading cultivation measure. So, shading from 20 days after transplanting can be recommended for application in Enshi cigar planting area.

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    Key technologies for full-process mechanized production of fresh soybean
    ZHANG Haipeng, DAI Wenhua, CHEN Jingjie, WANG Jue, ZHANG Yonggen
    2025, 66 (6):  1377-1380.  DOI: 10.16178/j.issn.0528-9017.20250291
    Abstract ( 13 )   HTML ( 2 )   PDF (1117KB) ( 22 )  

    To enhance the production efficiency of fresh soybean, research on key technologies for full-process mechanized production was conducted. Field experiments were carried out to investigate soybean varieties suitable for mechanical harvesting, harvester models, and technologies such as agricultural UAVs (unmanned aerial vehicles) for aerial seeding and plant protection. The results showed that Zhenong 6, Zhexian 9, and Gaoxiong 9 were more suitable for mechanized production. Among harvester models, the Wanke Baotian 4LDZ-48 and Yizhuo 4TD-16 fresh soybean harvesters exhibited better comprehensive performance, with Yizhuo 4TD-16 demonstrating more significant advantages in cost reduction and efficiency improvement. Among three sowing methods, manual sowing showed optimal comprehensive indicators; however, considering labor costs and sowing speed, aerial seeding was more ideal. Full-process mechanized production saved approximately 16 365 yuan·hm-2 in labor costs, demonstrating significant economic benefits and time savings, which is highly advantageous for optimizing farming schedules.

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    Effects of 6 soil treatments on continuous cropping obstacle of pepper in greenhouse
    JIN Ruo, GONG Jun, CHAI Weidong, KONG Wei, ZHOU Hanyue, ZHENG Jianbin
    2025, 66 (6):  1381-1384.  DOI: 10.16178/j.issn.0528-9017.20240319
    Abstract ( 13 )   HTML ( 4 )   PDF (1111KB) ( 24 )  

    In recent years, in order to ensure vegetable supply and improve vegetable planting efficiency, greenhouse vegetables have been rapidly developed and applied. However, due to unreasonable fertilizer and water management, unscientific cropping arrangements, continuous cropping obstacles of pepper in greenhouses have been increasing year by year, which was the most common in the western Zhejiang region. By setting up flooded greenhouses, soil conditioners, microbial composite agents 1, 2, soil conditioner+microbial composite agent 1, and soil conditioner+microbial composite agent 2 in areas with severe continuous cropping of pepper in greenhouses, the effects of different treatments on the control of continuous cropping obstacles of pepper in greenhouses were studied. The results showed that the treatment of adding microbial composite agent 2 had achieved the best effect on preventing and controlling continuous cropping obstacles of pepper in greenhouses, with a diseased incidence rate of 2.2%. Besides, the 667 m2 yield of peppers was 28.3% higher than that of the control group, with the output value increased by 3 456.3 yuan per 667 m2 compared with the control, and the net income increased by 2 426.3 yuan per 667 m2 compared with the control. Therefore, microbial composite agent 2 can be appropriately applied on continuous cropping of pepper in greenhouses.

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    Comparison of the application effects of two acidic soil conditioners in greenhouse vegetables
    BAI Songhua, NI Jinzhuang, PAN Haoliang, SHAO Yujing, LOU Ling, GU Wanfan, ZHU Shenghua
    2025, 66 (6):  1385-1388.  DOI: 10.16178/j.issn.0528-9017.20231205
    Abstract ( 13 )   HTML ( 2 )   PDF (1131KB) ( 26 )  

    Using a control group without soil conditioner application, this study compared the effects of two acidic soil conditioners on facility vegetables. The results showed that applying acidic soil conditioners improved the agronomic traits of Brassica rapa, increased its biomass yield, and enhanced planting benefits. Additionally, the soil pH value was elevated, mitigating soil acidification after application of acidic soil onditioners. In terms of vegetable yield and economic benefits, the application of Tuaobao soil conditioner demonstrated superior performance, achieving a 17.9% yield increase compared with the control and raising comprehensive benefits by 13 020.3 yuan·hm-2. The Tianshifu soil conditioner also showed notable effects, with a 12.8% yield increase and an additional benefit of 9 061.2 yuan·hm-2. Regarding soil pH value improvement, Tianshifu exhibited better efficacy, increasing soil pH value by 0.5 and reducing soil exchangeable acid content by 48.1% compared with the control. The study also revealed that applying soil conditioners in greenhouse vegetable cultivation contributed to controlling the occurrence of Brassica rapa clubroot disease.

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    Introduction performance of new loquat varieties Yingxue and Zhebai 28 in Lanxi
    MIAO Xiaodan, FAN Jun, ZHANG Qi, HU Jiahui, ZHOU Qingquan, LI Xiaoying
    2025, 66 (6):  1389-1393.  DOI: 10.16178/j.issn.0528-9017.20250151
    Abstract ( 15 )   HTML ( 4 )   PDF (1139KB) ( 28 )  

    To further develop the loquat industry in Lanxi, optimize the variety structure of loquats, extend the loquat marketing period, and achieve the effect of high quality and increased production. Lanxi Economic and Specialty Technology Promotion Center introduced two new loquat varieties Yingxue and Zhebai 28 from Zhejiang Academy of Agricultural Sciences. Through open-field cultivation, the introduction performance was observed. The results showed that the shoot emergence period of Yingxue and Zhebai 28 was delayed by 10-22 days compared with the local main variety Lanxi Baisha. The flowering period was from early October to early January. The budding period was delayed by about 21 days compared with Lanxi Baisha. Mature in mid to late May, more than 13 days later than Lanxi Baisha, Yingxue and Zhebai 28 both were late-maturing varieties.The growth characters and fruit quality characters (soluble solids contents, single fruit weight and edible rate, etc.) of the new varieties are better, with strong stress resistance, high yield, high popularity among consumers, and excellent comprehensive agronomic traits, which are suitable for promotion and planting in Lanxi.

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    Study on the cultivation characteristics of peach variety Chiyue in Jiaxing
    HU Binhui, CHEN Jianxiang, JIA Huijuan, LI Bin
    2025, 66 (6):  1394-1397.  DOI: 10.16178/j.issn.0528-9017.20250142
    Abstract ( 12 )   HTML ( 2 )   PDF (1112KB) ( 22 )  

    Using the Japanese peach variety Chiyue as the research object and Baifeng with similar maturity as the control, the main biological characteristics and fruit quality of Chiyue cultivated in Jiaxing Area were observed, measured, and analyzed. The results showed that Chiyue was characterized by round shape, with light yellowish-white peel and whole surface red. The flesh was sweet and juicy, with aroma, hard solute. The average fruit weight was 189.08 g, with a maximum weight of 205.30 g, and the soluble solid content(SSC) was approximately 13.91%. The fruit matured in early July, which was 1-3 days later than Baifeng, demonstrating early ripening, vibrant coloration, resistance to fruit cracking, high quality, and high yield potential. It can basically avoid typhoon damage. Furthermore, its superior quality and higher economic returns compared with mature peaches of the same period make it a valuable addition to optimizing the variety structure, and late-season peaches in Jiaxing Area.These attributes highlight the significant potential of Chiyue in promoting the development of the peach industry in Jiaxing Area.

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    Situation and quality evaluation of soil nutrients of Majia pomelo main producing area in Jiangxi Province
    SHAO Xinghua, CHEN Kai, CAI Jianbin, LIU Yanan
    2025, 66 (6):  1398-1403.  DOI: 10.16178/j.issn.0528-9017.20240774
    Abstract ( 11 )   HTML ( 2 )   PDF (1952KB) ( 26 )  

    Soil samples (0-30 cm) from ten Majia pomelo orchards were collected in four towns of Guangfeng District, Shangrao City, Jiangxi Province, in order to find out the soil fertility status of Majia pomelo orchards. The chemical properties of the soil pH value, organic matter, available nitrogen, available phosphorus, available potassium contents, and cation exchange capacity were measured, and soil fertility characteristics were evaluated by single factor evaluation and comprehensive evaluation. The results showed that average soil pH value in the main production area of Majia pomelo was 5.46, the average cation exchange capacity and organic matter content was moderate, which was 11.66 cmol·kg-1 and 22.64 g·kg-1, respectively. The average contents of available phosphorus and available potassium were 52.60 and 190.38 mg·kg-1, respectively, indicating abundant content. The average content of available nitrogen was 7.80 mg·kg-1, indicating a relative deficiency. The average comprehensive index (IFI) of soil fertility in 10 pomelo orchards was 0.53, 70% of sites classified as grade Ⅱ and Ⅲ, and 30% of pomelo orchards with lower fertility. Ten Majia pomelo orchards are generally lack of nitrogen nutrients, more nitrogen fertilizer should be applied. It is necessary to reduce the amount of phosphorus and potassium fertilizers appropriately, and application of more organic fertilizer is helpful to improve soil fertility and promote soil physical properties.

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    Effect of mixed sowing on component allocation and phenotypic traits of Agropyron mongolicum
    MA Yuan, XU Dongmei, LI Yongkang, SA Chunning, CHANG Yiran, KANG Rulong
    2025, 66 (6):  1404-1411.  DOI: 10.16178/j.issn.0528-9017.20240180
    Abstract ( 14 )   HTML ( 3 )   PDF (2940KB) ( 25 )  

    Mixed sowing of leguminous and gramineous forage plays an important role in improving forage quality and grassland productivity. A pot experiment was conducted to study the changes of individual biomass, component allocation, leaf traits and root traits of A. mongolicum based on the mixed sowing ratios of Agropyron mongolicum and Lespedeza potaninii with 12∶0 (M12N0), 8∶4 (M8N4) and 4∶8 (M4N8). The total individual biomass, root biomass, and root-shoot ratio of A. mongolicum were all higher under M8N4 treatment. The leaf biomass decreased with the decrease of the proportion of A. mongolicum in mixed sowing, and the highest was in the M12N0 treatment, with 0.50 g per plant, which was significantly higher than those under M8N4 and M4N8 treatments (P<0.05). The leaf length and area of A. mongolicum were the highest under M12N0 treatment and the lowest under M8N4 treatment. The specific leaf area, dry matter content and leaf thickness under M8N4 treatment were higher. The average root diameter of A. mongolicum was the highest under M12N0 treatment. The root length, specific root length, root surface area and root bifurcation number were the highest under M4N8 treatment, which were significantly higher than those under M12N0 and M8N4 treatments. The root volume and number of root tips under M8N4 treatment were high. With the increase of the proportion of A.mongolicum in mixed sowing the root length and specific root length of A. mongolicum decreased.Under different mixed sowing modes, the competitiveness of A.mongolicum can be enhanced by adjusting the component resource allocation and the plasticity of leaf traits and root traits, so as to ensure its growth and development. This study provides theoretical guidance for the restoration of degraded grasslands.

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    Effects of different rhizobia inoculations on the growth of Medicago sativa L.cv.Zhongmu 10
    CHEN Peng, SUO Mingchun, DONG Wenli, WEN Jiefu, ZHANG Yarong, WANG Zhenyu, FAN Hailong, SUN Jie
    2025, 66 (6):  1412-1419.  DOI: 10.16178/j.issn.0528-9017.20240223
    Abstract ( 11 )   HTML ( 2 )   PDF (1880KB) ( 23 )  

    Different rhizobia fungicide and fertilizer were used to inoculate Medicago sativa L.cv.Zhongmu 10, and the indicators of branching number, plant height, number of root nodules and biomass of Medicago sativa L. were measured at the early flowering stage to screen the fungicide and fertilizer that could improve the yield of Medicago sativa L.cv.Zhongmu 10. The results showed that, compared with the CK group, ACCC 17779 Chinese alfalfa rhizobia fungicide 5 mL (group A1) significantly increased the number of root nodules and underground biomass of Medicago sativa L., 10 mL (group A2) significantly increased the number of branches, plant height, the number of root nodules and underground biomass of Medicago sativa L.,15 mL (group A3) significantly increased the aboveground and underground biomass of Medicago sativa L.ACCC 17780 Chinese alfalfa rhizobia fungicide 5 mL (group B1) significantly increased the aboveground biomass of Medicago sativa L.,10 mL (group B2) significantly increased plant height, number of root nodules, aboveground and underground biomass of Medicago sativa L., and 15 mL (group B3) significantly increased underground biomass of Medicago sativa L. ACCC 03094 alfalfa rhizobial fungicide 5 mL (group C1) significantly increased the number of root nodules and underground biomass of Medicago sativa L, 10 mL (group C2) significantly increased the number of root nodules and underground biomass of Medicago sativa L., 15 mL (C3 group) significantly increased aboveground and underground biomass of Medicago sativa L. Huachuangjianong alfalfa rhizobia fungicide 5 mL (group D1) significantly increased the number of root nodules, 15 mL (group D3) significantly increased underground biomass of Medicago sativa L. Qiaonong rhizobia fertilizer 50 mL (group E1) and 100 mL (group E2) significantly increased the number of root nodules and root length, and 150 mL (E3 group) significantly increased the number of branches, root length, and aboveground and underground biomass of Medicago sativa L.. The above agents can be used as the main raw materials for the development of fungicide and fertilizer with the appropriate concentration for Medicago sativa L.cv.Zhongmu 10; Overall, Qiaonong rhizobia fertilizer has a lower cost and better cost-effectiveness compared with other agents.

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    High quality and efficient tissue culture and rapid propagation technology of Sedum formosanum N.E.Br.
    ZHAN Jing, SHEN Linjun
    2025, 66 (6):  1420-1424.  DOI: 10.16178/j.issn.0528-9017.20231228
    Abstract ( 13 )   HTML ( 2 )   PDF (2485KB) ( 22 )  

    This study utilized stem segments of Sedum formosanum N.E.Br. as experimental materials. Sterile seedlings were obtained through pretreatment and sterilization of explants. Subsequently, the explants were transferred to different proliferation and rooting media for gradient experiments, followed by comparative analysis to screen the optimal tissue culture formulation. The results showed that spraying the mother plants with 800-1 000 times diluted 50% carbendazim solution every other day in one week prior to explant collection effectively reduced endophytic bacteria and improved the survival rate of sterile explants. The highest sterile survival rate of explants (76%) was achieved by adding 0.2% plant tissue culture antimicrobial agent to the induction medium, combined with sterilization using alcohol for 30 s and 0.1% HgCl2 for 8 min. The proliferation medium MS + 1.5 mg·L-1 6-BA + 0.1 mg·L-1 NAA + 30 g·L-1 sucrose + 8 g·L-1 agar demonstrated superior proliferation effects, with a proliferation coefficient of 21.4. Sedum formosanum N.E.Br. exhibited excellent rooting performance on 1/2 MS medium, achieving a 100% rooting rate. This study established a tissue culture rapid propagation system for Sedum formosanum N.E.Br., laying a foundation for its industrialized production and enriching China's groundcover plant species resources.

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    Analysis of the pathogens of gray mold on leek in Zhejiang Province
    WU Jun, FANG Li, KE Jingjing, WANG Hanrong
    2025, 66 (6):  1425-1428.  DOI: 10.16178/j.issn.0528-9017.20240227
    Abstract ( 10 )   HTML ( 2 )   PDF (4229KB) ( 25 )  

    Leek is a small crop that creates high economic value. Gray mold has become one of the important diseases restricting the development of leek industry. In this study, these diseased plants were collected from Yuhang District, Hangzhou City and Longyou County, Quzhou City, the pathogens species of gray mold on leek were identified based on the molecular identification, and pathogenicity determination. The results showed that 92 strains were isolated, which consisted of Botrytis squamosa and Botrytis cinerea. Botrytis squamosa were extremely dominant. The results can provide a reference for the control and prevention of gray mold on leek.

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    Pathogen identification, biological characteristics and fungicides screening of leaf spot disease of Saccharum officinarum
    ZHANG Yuqing, WU Jun, FANG Li, JIN Wenxuan, WANG Hanrong
    2025, 66 (6):  1429-1432.  DOI: 10.16178/j.issn.0528-9017.20240266
    Abstract ( 12 )   HTML ( 1 )   PDF (3768KB) ( 29 )  

    The diseased leaves and stems of Saccharum officinarum were collected from Wenling County, Taizhou City, Zhejiang Province. The results showed that the pathogens were identified as Curvularia lunata based on pathogen isolation, Koch postulates and molecular identification. The effect of carbon source, nitrogen source, temperature, light and pH value on growth of GZWB21 were determined, these results suggested that strain GZWB21 grew well on six carbon sources, the optimal carbon source and nitrogen source of GZWB21 were sucrose and yeast powder, respectively. The optimal temperature was 35℃, the optimal pH value was 8. Besides, 43% fluoromystrobin suspension, 450 g·L-1 prochloraz aqueous emulsion and 50% acetimidine aqueous dispersible granules had a strong inhibitory effect on GZWB21, with EC50 of 0.003 8、0.002 7 and 0.124 0 mg·L-1. This study can provide a reference for the prevention and control of leaf spot disease of Saccharum officinarum.

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    The biological characteristics of Phenacoccus solenopsis Tinsley on potato leaves
    ZHI Fuying, LIU Xiaotong, ZHANG Juan, FU Kaiyun, YANG Dong, GAO Jiancheng, HUANG Jun, LYU Yaobin
    2025, 66 (6):  1433-1436.  DOI: 10.16178/j.issn.0528-9017.20240132
    Abstract ( 11 )   HTML ( 2 )   PDF (2829KB) ( 22 )  

    In order to elucidate the biological characteristics of Phenacoccus solenopsis Tinsley on host potato leaves, this study conducted indoor feeding observations to investigate the life history, developmental changes, physiological transitions, and reproductive features of P. solenopsi on potato leaves. The findings revealed that P. solenopsi reproduces through amphitrogenesis, with distinct developmental periods for female and male insects. Female development encompasses egg, larvae, and adult stages, while male development includes egg, larvae, pre-pupae, pupae, and adult stages. Furthermore, the developmental time of female and male insects varies depending on their developmental periods. The developmental period for male larvae and pupae was 14-23 days, with a total lifespan of 18-25 days; whereas the developmental period for female larvae was 12-21 days, with a total lifespan of 26-54 days. Additionally, the dark coloration of the dorsal black spots in both male and female insects can serve as indicators for distinguishing each instar.

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    Study on the effect of 9 fungicides on the pregnancy of Zizania latifolia
    CHEN Liping, CHEN Shuting, XU Biqi, LI Yipeng, CANG Tao, LIU Caixiu, ZHANG Changpeng, WANG Xiangyun
    2025, 66 (6):  1437-1441.  DOI: 10.16178/j.issn.0528-9017.20240850
    Abstract ( 15 )   HTML ( 3 )   PDF (1166KB) ( 25 )  

    Zizania latifolia is an important aquatic vegetable in China. However, there are few registered pesticides for it, leading to a common issue of off-label pesticide use. The inappropriate use of fungicides can easily result in reduced pregnancy rates and other phytotoxicities in Z. latifolia. This study selected nine commonly used fungicides for field and laboratory experiments to evaluate their impact on Z. latifolia pregnancy. The field experiment results showed that Z. latifolia pregnancy stage is not suitable for the use of azoxystrobin, triadimefon, flutriafol, and tebuconazole. After three consecutive applications, the pregnancy rates were only 39.44%, 27.76%, 21.64%, and 10.37%, respectively. It is also not advisable to use tebuconazole and azoxystrobin 30 days before the pregnancy stage of Z. latifolia, as the pregnancy rates were only 30.77% and 11.70%, respectively after a single application. The laboratory experiment results indicated that the EC50 values of the nine fungicides for inhibiting the growth of the Ustilago esculenta haploid strain MT3 ranged from 0.017 5 mg·L-1 to 8.470 6 mg·L-1, with tebuconazole having the lowest EC50 value. However, the EC50 value ranking of the nine fungicides did not align with their field performance, suggesting that the impact of fungicides on Z. latifolia pregnancy in the field is a comprehensive result of the actual dosage acting on the U. esculenta and possible metabolic products.

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    Inhibitory effect of winter spraying calcium based composite polymers on kiwifruit canker
    WANG Zhengqian, WANG Xiao, HE Gang, HE Zimei, DU Chunxue, LIU Chunhong, LI Mingjie, LI Chunhua
    2025, 66 (6):  1442-1449.  DOI: 10.16178/j.issn.0528-9017.20240231
    Abstract ( 13 )   HTML ( 2 )   PDF (6420KB) ( 21 )  

    The cell wall surface and intercellular layer were filled with reagents after spraying calcium based composite polymer, which could significantly change the overwintering performance of kiwifruit. The cut and wound of kiwifruit branch were not frozen and thawed, which reduced the alternate freeze-thaw injury,with the field incidence did not exceed 2.18%, and the prevention effect was not less than 84.5%. The field control effect was better when Cu (OH) 2 was added to the composite polymer. The incidence and prevention effect of kiwifruit sprayed with calcium based composite polymer adding Cu (OH) 2once were 1.34% and 90.5%, respectively, while incidence and prevention effect of kiwifruit sprayed with calcium based composite polymer adding Cu (OH)2 twice were 0.96% and 93.1%, respectively. The net photosynthetic rate, stomatal conductance, transpiration rate and total chlorophyll content of the susceptible kiwifruit plants after spraying calcium based composite polymer adding Cu (OH)2 for 3 months were 48.1%, 57.3%, 24.8% and 69.9% higher than those after spraying for 1 month, respectively. The intercellular CO2 concentration was reduced by 16.0%. When there was white bacterial pus, calcium based composite polymer adding Cu (OH)2 could be sprayed for treatment, with the cure rate not less than 87.5%.

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    Study on selectivity of Myzus persicae(Sulzer)to different tobacco varieties
    WANG Yale, XUE Bingjie, SUN Jiping, LI Xuejun, SUN Huan
    2025, 66 (6):  1450-1455.  DOI: 10.16178/j.issn.0528-9017.20250153
    Abstract ( 13 )   HTML ( 2 )   PDF (1289KB) ( 23 )  

    In order to provide a valuable resistance source for the cultivation of Myzus persicae(Sulzer) resistant varieties, 373 tobacco germplasm resources were evaluated by in vitro leaf method. The selectivity of Myzus persicae (Sulzer) to different tobacco varieties was analyzed, and the resistance of Myzus persicae(Sulzer) was evaluated according to the evaluation method of Myzus persicae(Sulzer) resistance of tobacco varieties. The results showed that the Myzus persicae(Sulzer) had feeding selectivity to different tobacco varieties. Thirteen varieties with low selectivity to tobacco aphids were screened out, among which Shuba 2139, Shuba 2136, Heimiaomaoyan, Huangmiao 2230, Baijiancao 2489, Shuba 2129, Shubalaomuji 2113 and huangmiaosongbian were local varieties, and Jiyan 5, Yuyan 13, Y102, Longjiang 911 and 664-01 were domestic bred varieties. Seventy-eight varieties with lower selectivity were screened out. These varieties can be used as candidate materials for further identification of Myzus persicae(Sulzer) resistance. The number of tobacco with medium selectivity was the highest (32.44%), followed by tobacco varieties with high selectivity to Myzus persicae(Sulzer)(28.42%). The proportion of varieties with low selectivity to Myzus persicae(Sulzer) was small, and the genetic basis was narrow.

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    Analysis of the effect of five kinds of chemical agents on the emergency control of Phenacoccus solenopsis Tinsley
    QIAN Cheng, CHEN Zengqian, LIU Xiaotong, WEI Haohua, ZHANG Juan, WANG Ke, YU Xianwen, HUANG Jun
    2025, 66 (6):  1456-1459.  DOI: 10.16178/j.issn.0528-9017.20240765
    Abstract ( 11 )   HTML ( 2 )   PDF (1386KB) ( 20 )  

    Phenacoccus solenopsis Tinsley poses a significant threat as an invasive pest in our region with severe implications for cotton, eggplant, and other plant species. Its formidable challenge for prevention and management stems from its large spawning amount, strong reproduction ability and fast development rate, leading to extensive crop damage. To address timely and effective emergency response strategies against P. solenopsis Tinsley, we compared and analyzed the prevention and control effects of 5 chemical agents, including 24% spirochaete·pyriproxyfen, 33% spiroxamine, 28% pukechu, 40% fluoxetine·spirotrifonate and 22% spiroworm·Thiacloprid on this pest. The results showed that 22% Spiroworm·Thiacloprid had the best control effect on P. solenopsis Tinsley, and the control effect of 1∶1 000 diluent was up to 90.0% after 2 days.

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    Analysis of insect community structure in rice field under monitoring lamp
    HONG Zhihui, KE Hanyun, ZHAO Shuaifeng, HU Xuanxiang, HUANG Yanping, XU Yunhong, PAN Xiaozhi, QIU Zhiqiang, WENG Bingxin, XIE Shengwang
    2025, 66 (6):  1460-1466.  DOI: 10.16178/j.issn.0528-9017.20240865
    Abstract ( 11 )   HTML ( 2 )   PDF (1325KB) ( 24 )  

    In order to understand the insect community structure during the growth period of rice in the field and explore its occurrence rule and damage characteristics, the insect community under the monitoring lamp was investigated in the rice field of Jiande City, Zhejiang Province from 2014 to 2023. In this study, the natural composition, dominant species, functional composition of the insect community and comparison of insect communities at different time were analyzed. The data showed that the insect community under the monitoring lamp in the rice field consisted of 14 orders and 81 species, among which Lepidoptera had the largest number, accounting for 30.86%, followed by Homoptera and Coleoptera, accounting for 17.28% and 14.81%, respectively. According to the nutrient level, the insect community can be divided into four functional groups, in which the phytophagous insects occupy the dominant position, with the number of species accounting for 72.83% and the number of individuals accounting for 32.79%. Among the phytophagous insects, the main pests that pose a threat to rice accounted for 48.14% of the total species, and these pests are numerous and large in number, causing the most serious damage to the rice field. The top 7 dominant insects in the community were Anopheles sp., Sogatella furcifera, Lasius niger, Stenchaetothrips, Colaphellus bowringi, Chilo suppressalis and Nilaparvate lugens, which accounted for 78.77% of the total number of the community and played a decisive role in the community structure and community environment. According to the time division, the diversity and evenness of insect community in rice field showed a decreasing trend year by year, but the diversity and evenness of insect community in rice field increased abnormally in 2020-2021, and the diversity index (H') and evenness (J) were the largest in 10 years.

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    Study on the control efficacy and toxicity of eight pesticides against greenhouse tomato whitefly
    WU Cong, CHEN Jiahao, SHAO Ting, DAI Dejiang
    2025, 66 (6):  1467-1470.  DOI: 10.16178/j.issn.0528-9017.20240024
    Abstract ( 13 )   HTML ( 2 )   PDF (1136KB) ( 24 )  

    To promptly understand the development of resistance in whitefly, a major pest in tomato greenhouses in Lanxi City, to conventional pesticides, and to screen for highly effective, safe, and low-toxicity pesticides for controlling greenhouse whiteflies, laboratory toxicity bioassays and field trials were conducted to compare the toxicity and control efficacy of eight pesticides against adult whitefly. The results revealed significant differences in toxicity among the tested pesticides. Among them, 25% nitenpyram SP maintained relatively high toxicity against adult whiteflies, with an average LC50 of 1.58 mg·L-1. In contrast, adult whitefly exhibited low sensitivity to 22% thiacloprid WG and 1.3% matrine AS, particularly the latter, which had an average LC50 of 75.92 mg·L-1. Field trial results demonstrated that 25% Nitenpyram SP, 20% dinotefuran WP, 70% imidacloprid WG, and 10% flonicamid WG all showed superior control efficacy at 7 and 14 days after application. Given the significant variation in the susceptibility of greenhouse whitefly adults to different pesticides and combined with laboratory toxicity data, 25% nitenpyram SP, 20% dinotefuran WP, 70% imidacloprid WG, and 10% flonicamid WG are recommended as effective pesticides for controlling whitefly in greenhouses in Lanxi City.

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    Monitoring of cadmium content in sweet potato based on hyperspectral analysis
    CHEN Yu, SHU Xing, BAI Jiacheng, LYU Zunfu
    2025, 66 (6):  1471-1476.  DOI: 10.16178/j.issn.0528-9017.20240192
    Abstract ( 13 )   HTML ( 5 )   PDF (3805KB) ( 22 )  

    The rapid detection of cadmium is of great significance for the high-quality and safe production of sweet potatoes. This experiment used five sweet potato varieties, Xinxiang, Shulv 1, Guangshu 87, Zheshu 13, and Shangshu 19, for pot cultivation. By setting five different contents of cadmium treatment gradients, the characteristics of cadmium absorption and accumulation in sweet potatoes were explored. The results showed that the heavy metal cadmium content in sweet potato leaves and root tubers showed an upward trend with the increase of soil cadmium content. By analyzing the spectral reflectance characteristics of sweet potato leaves, key spectral bands closely related to cadmium content were identified, and two sensitive bands (830 and 910 nm) with the strongest correlation with leaf cadmium content and normalized vegetation index (NDVI) were determined within these bands. A quantitative estimation model for heavy metal content in sweet potato leaves was constructed based on the NDVI index,achieving real-time monitoring and accurate diagnosis of cadmium content in sweet potato plants.

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    Inhibition of key enzymes in glucose and lipid metabolism by extracts from Ganoderma lucidum at different growth stages
    ZHAO Ruimeng, WU Zhipeng, LAN Liuxin, XU Zhouwei, CHEN Jinrong, LYU Guoying, WANG Mengyu, ZHANG Zuofa
    2025, 66 (6):  1477-1481.  DOI: 10.16178/j.issn.0528-9017.20231212
    Abstract ( 12 )   HTML ( 2 )   PDF (4684KB) ( 26 )  

    Ganoderma lucidum is a highly valued medicinal and edible mushroom rich in bioactive components with broad pharmacological activities. This study systematically compared the inhibitory effects of ethanol extracts and crude polysaccharides from G. lucidum at different growth stages on the activity of key enzymes involved in glucose and lipid metabolism. The results revealed that ethanol extracts from the cap-opening stage exhibited the highest inhibition rates on α-amylase and α-glucosidase, while those from the primordial stage showed the strongest inhibition on lipase. For crude polysaccharides, the maximum inhibition rates on α-amylase, α-glucosidase, and lipase were observed during the primordial, cap-opening, and mature stages, respectively. These findings provide critical insights for optimizing the harvesting period of G. lucidum and guiding its product development.

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    Determination of 43 kinds of pesticide residues in the samples of planting proficiency testing
    CHEN Ziping, HE Mingshan, ZHANG Xuehong
    2025, 66 (6):  1482-1488.  DOI: 10.16178/j.issn.0528-9017.20240286
    Abstract ( 13 )   HTML ( 2 )   PDF (2947KB) ( 26 )  

    Gas chromatography is widely used in county-level agricultural product quality and safety testing institutions, and was used to detect 43 pesticide residues in pak choi samples numbered LAB-156 in the proficiency testing of pesticide residue detection for planting products in Guangdong Province in 2023. The results showed that five pesticides such as triazophos, acephate, methidathion, triadimefon and fenvalerate were added to the positive test samples of pak choi, and the other 38 kinds of pesticides were not detected. The detection values of quantitative detection were 0.086 8, 0.084 9, 0.118 0, 0.102 0 and 0.099 2 mg·kg-1, respectively. The recovery rates of pesticide residues in the quality control standard samples of the same batch were between 79.0%-99.2%. Combined with the spiked values announced by the organizer at a later stage, the recovery rates of triazophos, acephate, methidathion, triadimefon and fenvalerate in the test samples were 108.5%, 84.9%, 98.3%, 102.0% and 99.2%, respectively, which were all within the qualified range of 70.0%-120.0%, and there was no missed or multiple detection. It can be judged that the test results of 43 kinds of pesticide residues in the numbered LAB-156 samples were qualified. The results of this study can be used as a reference for the determination of pesticide residues in planting products by county-level agricultural product quality and safety testing institutions.

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    Optimization of extraction process of gardenia yellow pigment by response surface methodology
    LI Qunhe, SU Fengxian, ZOU Ying, ZHOU Yanzi
    2025, 66 (6):  1489-1495.  DOI: 10.16178/j.issn.0528-9017.20250205
    Abstract ( 13 )   HTML ( 2 )   PDF (6393KB) ( 24 )  

    In this study, the extraction technology of gardenia yellow pigment was optimized through response surface test design. Based on single-factor experimental results, a Box-Behnken response surface methodology was employed to design a 4-factor 3-level experimental scheme. With gardenia yellow pigment extraction yield as the response variable, the investigation focused on pulsed electric field intensity, ethanol volume fraction, solid-to-liquid ratio, and pulse times as critical process parameters. According to the regression equation, the optimal extraction conditions were obtained as follows: Electric field strength was 25 kV·cm-1, ethanol volume fraction was 28%, solid-liquid ratio was 1∶18 g·mL-1, pulse times was 8. Under these conditions, the comprehensive score was(9.13±0.53)%, which was basically consistent with the theoretical prediction value of 9.18% by the regression equation. In conclusion, the high voltage pulsed electric field assisted extraction process optimized by response surface methodology is simple and reliable in the extraction of gardenia yellow pigment, providing a theoretical basis for large-scale extraction of gardenia yellow pigment.

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    Isolation, identification, and drug resistance analysis of Streptococcus equi subsp. zooepidemicus from guinea pigs
    CHEN Qiaodan, CHEN Yijie, CHENG Jufen, YUAN Xiufang, YU Bin, SU Fei, YE Shiyi, XU Lihua, ZHANG Hui, LI Junxing
    2025, 66 (6):  1496-1500.  DOI: 10.16178/j.issn.0528-9017.20240202
    Abstract ( 13 )   HTML ( 2 )   PDF (2340KB) ( 22 )  

    In order to identify the causative agent of sudden death of guinea pigs at a farm, clinical observation, pathological dissection and bacterial isolation were conducted on guinea pigs suspected of bacterial infection. Gram staining, 16S rDNA sequencing and antimicrobial susceptibility test were performed following bacterial isolation. The results showed that the same pathogenic bacteria could be isolated from multiple organs of dead guinea pigs, which had obvious β- hemolytic rings, Gram positive staining, and were arranged in pairs or short chains. The 16S rDNA sequence of the isolated bacterial strain had a high homology of 99.77% with that of Streptococcus equi subsp. zooepidemicus (MK614224). The results of antimicrobial susceptibility test showed that the isolated bacterial strain was sensitive to 4 drugs, including ceftiofur and doxycycline; Moderate sensitivite to erythromycin and ciprofloxacin; Resistant to 15 drugs including cefotaxime and penicillin. The results of this experiment confirmed that Streptococcus equi subsp. zooepidemicus was the pathogen causing sudden death of guinea pigs, and the isolated strain showed strong drug resistance, providing a reference for the prevention and control of Streptococcus equi subsp. zooepidemicus.

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    Effects of combined application of bio-organic fertilizer and microbial agents on soil carbon composition, enzyme activity, and carbon process in newly cultivated paddy fields
    LI Youjie, CHEN Zihan, DU Jiamei, CHEN Xiani, ZHANG Yue, YANG Qiyu, LI Zixuan, ZHAO Mingxing, ZHANG Xiancui, LI Peng, ZHOU Wei, ZHANG Yuliang, HU Lihua, WU Choufei
    2025, 66 (6):  1501-1505.  DOI: 10.16178/j.issn.0528-9017.20240524
    Abstract ( 12 )   HTML ( 3 )   PDF (3014KB) ( 24 )  

    Exploring the effects of combined application of bio-organic fertilizer and microbial agents on soil organic carbon and enzyme activity is of great significance for rapid improved soil fertility and sustainable production in newly cultivated paddy fields. Selecting newly cultivated paddy fields in hilly areas under the "balance of ccupation and compensation"policy in Quzhou City,Zhejiang Province as the study object, five different treatments were set up using bio-organic fertilizer combined with different gradient microbial agents: Treatment 1 (bio-organic fertilizer+per 667 m2 30 kg microbial agent), Treatment 2 (bio-organic fertilizer+per 667 m2 35 kg microbial agent), Treatment 3 (bio-organic fertilizer+per 667 m2 60 kg microbial agent), Treatment 4 (bio-organic fertilizer+per 667 m2 50 kg microbial agent), and Treatment 5 (bio-organic fertilizer+per 667 m2 60 kg microbial agent). Soil samples of the plough layer were collected during rice harvest period to analyze the soil organic carbon content and the characteristics of enzyme acticity. The results showed that as the microbial agent gradient increased, the organic carbon content of newly cultivated paddy soil showed an increasing trend, but there was no significant difference between the treatments. The increase in microbial agent gradient improved the activity of soil carbon cycling enzymes to some extent. Compared with treatment 1, treatment 4 and 5 significantly increased cellulase activity (P<0.05). High microbial agent gradients (treatment 4 and 5) increased soil carbon processes, but there was no significant difference between treatment 4 and 5. There was a significant positive correlation (P<0.05) between soil carbon processes and phenolic oxidase and cellulase. In summary, as the gradient of combined bio-organic fertilizer and microbial agents increases, it can improve soil organic carbon content and enzyme activity, promote soil carbon cycling process, which will be beneficial for the sustainable utilization of newly cultivated paddy fields under long-term continuous improvement.

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    Research progress on the effects of melatonin on plant growth and development under stress
    ZHANG Dongdong, ZHOU Cece, SHANGGUAN Yibo, YANG Lina, LIU Xiaoen, CHENG Qiongmin, BAO Xiaorong, YAN Yali, WANG Hui
    2025, 66 (6):  1506-1510.  DOI: 10.16178/j.issn.0528-9017.20240245
    Abstract ( 15 )   HTML ( 4 )   PDF (1169KB) ( 20 )  

    Melatonin (MT), as a type of hormone substance, has been proven to be widely present and participate in the growth and development of plants. It plays an important role in the continuous adaptation of plants to new environments. In recent years, scholars at home and abroad have conducted comprehensive research on the involvement of MT in plant resistance to different stress such as drought, salt, heavy metals, high temperatures and low temperatures. This article introduced the natural synthesis pathway of MT in plants, the mechanism of MT in plant stress resistance, and the relationship between MT content and different stresses by referring to existing research results. It also proposed future research directions and prospects for MT in stress resistance.

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    Research progress and development proposals of microbial seed coating agents
    ZHANG Keying, ZHANG Jiaxuan, SUO Xinge, LIU Xinyue, XUE Qianwen, HE Yanhui, WU Zhansheng, FAN Zhongdi
    2025, 66 (6):  1511-1517.  DOI: 10.16178/j.issn.0528-9017.20240521
    Abstract ( 13 )   HTML ( 2 )   PDF (1194KB) ( 24 )  

    Microbial seed coating agents, as a high-tech that drives the development of the seed industry and promotes increased agricultural yields and abundant harvests, have been widely used worldwide, and have become a mainstream in agricultural production. This paper described the current status of domestic and international research on microorganisms, film-forming agents, adhesives and coating methods of microbial seed coating agents, analyzed their impacts on the performance of microbial seed coating agents, and explored the impacts of biotic and abiotic stresses with the growth of the seed coating agents on crops and so on. At the same time, in combination with the main problems of agricultural development of seed coating agents in China, corresponding solutions and development proposals are put forward, with a view to providing reference for future in-depth research on seed coating.

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    Distribution and biodiversity effects of invasive plants in important wetlands in southern Jiangsu Province
    WANG Zhe, ZHANG Hanbo, WANG Miao, ZHANG Qingqing, LIU Maosong
    2025, 66 (6):  1518-1525.  DOI: 10.16178/j.issn.0528-9017.20240273
    Abstract ( 12 )   HTML ( 2 )   PDF (2112KB) ( 20 )  

    In order to clarify the relationship between alien invasive plants and diversity of native plant in typical wetlands in the Yangtze River and its southern regions in Jiangsu Province, the typical wetlands in Taihu Lake and Shijiu Lake were selected as the research areas for plant investigation, and the correlation between the richness of alien invasive plants and the richness of native plants, as well as the invasion coefficient of alien invasive plants and the diversity of native plant species were analyzed. The results showed that a total of 625 species of higher vascular plants were found in the survey, including 28 species of invasive plants belonging to 21 genera and 12 families. Among them, Compositae was the dominant family, with the majority being annual herbs. Solidago canadensis and Veronica persica had a high frequency of invasion and a wide distribution. Coreopsis basalis and Chenopodium glaucum had the greatest dominance. The alien invasive plants in the study area significantly reduced the diversity of native plants, and the invasion coefficient of alien invasive plants was significantly negatively correlated with the richness, the Shannon-Wiener index, the Simpson index, and the Pielou index of native plants(P < 0.05).

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    Study on accurate prediction of forest volume based on ARMA model
    GAO Changjian, WANG Hailong, LAN Yihan, WANG Shihong
    2025, 66 (6):  1526-1530.  DOI: 10.16178/j.issn.0528-9017.20250127
    Abstract ( 12 )   HTML ( 3 )   PDF (1154KB) ( 22 )  

    Forest volume is a crucial indicator for assessing forest resource abundance, ecosystem health, and carbon sequestration capacity. Accurate predictions of forest volume are essential for sustainable forest management and carbon neutrality planning. This study comprehensively compared the performance of four methods for predicting forest volume: the auto regressive moving average(ARMA) model combined with the partial least squares method (ARMAP), the ordinary least squares method (ARMAO), the grey system theory (GST), and the BP neural network (BPNN). Using forest land area and forest volume data from Chengfeng Town, Yongtai County, Fuzhou City, from 1986 to 1999, models were developed and validated with data from 1998 and 1999. The results indicate that the highest prediction accuracy by effectively filtering colored noise, with significantly lower residual variance and prediction errors compared with other models. Further validation using Zhejiang Province's 2020 forest volume data demonstrated the model's strong generalization ability. This study shows that the ARMAP model can achieve high-precision and efficient forest volume predictions under complex disturbance scenarios, providing methodological support for forest resource management and carbon sink evaluation.

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    Research progress on carbon sequestration capacity and influencing factors of bamboo forests
    ZHANG Hua, ZHAN Weiming, XU Qianliang, TU Chunbao, LI Jinhui, YUE Cong, WANG Yuhang, WANG Zhe
    2025, 66 (6):  1531-1535.  DOI: 10.16178/j.issn.0528-9017.20240246
    Abstract ( 12 )   HTML ( 3 )   PDF (1134KB) ( 23 )  

    Global climate change is an urgent problem that humanity needs to face, and it concerns the future of humanity. Low carbon economy is an important way to address global climate change. Forests account for 46.3% of the total global carbon pool, and bamboo forest carbon storage occupies an important position in the entire forest carbon storage, playing an indispensable role in carbon sequestration and emission reduction. This article summarizes and analyzes the carbon sequestration and emission reduction capacity of bamboo forests and their influencing factors, aiming to provide reference and assistance for addressing global climate change, exploring methods for balancing ecological and economic benefits of bamboo forests, and conducting subsequent research on the comprehensive impact of multiple factors on bamboo forests.

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    Study on brand construction and promotion path of characteristic agricultural products in Lishui
    LIAO Xiaoli, LIANG Ziyi, LIAN Yan
    2025, 66 (6):  1536-1541.  DOI: 10.16178/j.issn.0528-9017.20250094
    Abstract ( 15 )   HTML ( 2 )   PDF (1144KB) ( 26 )  

    The branding construction of characteristic agricultural products is the main way to achieve rural revitalization. Relying on its excellent ecological environment and abundant agricultural resources, Lishui City has discovered and cultivated a large number of characteristic agricultural products. Through the implementation of the agricultural product brand strategy, it has created well-known brands such as "Lishuishangeng", promoting the organic integration of local ecological industries and increasing farmers' income. However, due to the limitations of regional characteristics, the brand construction of agricultural products in Lishui City has problems such as relatively small industrial scale, low standardization degree, short industrial chain, and insufficient brand driving force, which have led to weak market competitiveness and low added value of Lishui's characteristic agricultural products. In order to solve the above-mentioned problems, Lishui City has adopted a series of strategies such as strengthening the construction of regional public brands, promoting the integrated and coordinated development of industries, building a multi-channel marketing system, and improving quality and ensuring quantity. Remarkable achievements have been made in enhancing brand influence, adding value to agricultural industries, increasing farmers' income, and converting ecological benefits. Overall, the improvement paths for the brand of Lishui's characteristic agricultural products mainly include expanding the marketing system, driving industrial upgrading through the market, creating characteristic leading products, integrating multiple industries, and building a cluster industrial development model, etc. The success of brand promotion has driven the development of the agricultural industry in Lishui area, increased farmers' income, and provided a successful model for rural revitalization in mountainous areas of China.

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    Application of artificial intelligence in enzyme engineering
    WU Jianxiong, BAO Yufeng
    2025, 66 (6):  1542-1550.  DOI: 10.16178/j.issn.0528-9017.20250132
    Abstract ( 9 )   HTML ( 2 )   PDF (2652KB) ( 22 )  

    As an efficient and specific biocatalyst, enzymes are widely used in industry, medicine and environmental protection. However, the limitations of natural enzymes in terms of stability, catalytic activity, and selectivity limit their industrial applications. Enzyme engineering mainly adopts a variety of strategies such as rational design, directed evolution, semi-rational design, and artificial intelligence-aided design. Among them, directed evolution simulated the process of natural selection and screened out enzyme mutants with better performance. The rational design is based on the known structure and active site of the enzyme and is precisely modified. Although traditional enzyme engineering methods have achieved some success, they face challenges such as large sequence space, high experimental cost and scarce data. In recent years, the introduction of artificial intelligence technology has brought revolutionary breakthroughs to enzyme engineering. AI technology has shown significant advantages in protein structure prediction, function optimization, and mutant screening, which greatly expands the ability to explore enzyme sequence space and improves the efficiency of enzyme molecular modification. However, the application of AI technology in enzyme engineering still faces challenges such as data scarcity, model generalization, and experimental verification efficiency. In the future, with the improvement of computing power and the advancement of experimental technology, AI technology is expected to achieve higher precision and wider application in enzyme design, providing strong support for green industry and sustainable development.

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    Study on the integrated development of agriculture and tourism in county areas
    XU Jie, WANG Lei
    2025, 66 (6):  1551-1558.  DOI: 10.16178/j.issn.0528-9017.20250600
    Abstract ( 9 )   HTML ( 2 )   PDF (1179KB) ( 21 )  

    The integration of agriculture and tourism can effectively promote rural revitalization, better solve the problems of agriculture, rural areas, and farmers, and has practical significance for regional economic and income growth, as well as ecological construction. This paper is based on relevant literature and theory, and uses a coupling coordination degree model to calculate the coupling coordination of agriculture and tourism in 132 counties in Zhejiang Province, Anhui Province, and Chongqing City from 2012 to 2020. The results show that the coupling degree of most counties is in the stage of high-quality coordination, indicating a high degree of closeness between the agricultural and tourism industries; Most counties have changed from transitional coordination zone to moderate coordination zone, indicating that although agriculture and tourism are closely related and developing well, the integration level is still at a low level. The integrated development of agriculture and tourism has not been put forward for a long time, and the road of integrated development of various regions still needs to be explored in practice.

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    Current situation and development strategies of vegetable industry in Zhejiang Province
    WANG Shuwen, SHI Yisen, XIE Shuzhen, LIU Lingmin, SHENG Kai, ZHOU Jiayan, HU Zhangjian
    2025, 66 (6):  1559-1564.  DOI: 10.16178/j.issn.0528-9017.20240328
    Abstract ( 13 )   HTML ( 3 )   PDF (1135KB) ( 20 )  

    This paper collected and organized data related to the vegetable industry in Zhejiang Province over the past decade. Based on agricultural industry theory, regional advantages, and comparative advantage theory, it analyzed the current development status of the vegetable industry in Zhejiang Province, compared data changes, and obtained the characteristics of the evolution of the vegetable industry layout in Zhejiang Province. Based on the analysis of the comparative advantages and existing problems of the vegetable industry, this paper proposed strategies and suggestions to promote the high-quality development of the vegetable industry in Zhejiang Province.

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