Effects of combined application of bio-organic fertilizer and microbial agents on soil carbon composition, enzyme activity, and carbon process in newly cultivated paddy fields

doi:10.16178/j.issn.0528-9017.20240524

Abstract

Abstract:

Exploring the effects of combined application of bio-organic fertilizer and microbial agents on soil organic carbon and enzyme activity is of great significance for rapid improved soil fertility and sustainable production in newly cultivated paddy fields. Selecting newly cultivated paddy fields in hilly areas under the "balance of ccupation and compensation"policy in Quzhou City,Zhejiang Province as the study object, five different treatments were set up using bio-organic fertilizer combined with different gradient microbial agents: Treatment 1 (bio-organic fertilizer+per 667 m² 30 kg microbial agent), Treatment 2 (bio-organic fertilizer+per 667 m² 35 kg microbial agent), Treatment 3 (bio-organic fertilizer+per 667 m² 60 kg microbial agent), Treatment 4 (bio-organic fertilizer+per 667 m² 50 kg microbial agent), and Treatment 5 (bio-organic fertilizer+per 667 m² 60 kg microbial agent). Soil samples of the plough layer were collected during rice harvest period to analyze the soil organic carbon content and the characteristics of enzyme acticity. The results showed that as the microbial agent gradient increased, the organic carbon content of newly cultivated paddy soil showed an increasing trend, but there was no significant difference between the treatments. The increase in microbial agent gradient improved the activity of soil carbon cycling enzymes to some extent. Compared with treatment 1, treatment 4 and 5 significantly increased cellulase activity (P<0.05). High microbial agent gradients (treatment 4 and 5) increased soil carbon processes, but there was no significant difference between treatment 4 and 5. There was a significant positive correlation (P<0.05) between soil carbon processes and phenolic oxidase and cellulase. In summary, as the gradient of combined bio-organic fertilizer and microbial agents increases, it can improve soil organic carbon content and enzyme activity, promote soil carbon cycling process, which will be beneficial for the sustainable utilization of newly cultivated paddy fields under long-term continuous improvement.

Key words: newly cultivated paddy fields, bio-organic fertilizer, soil organic carbon, enzyme activity, carbon process

CLC Number:

S158.1

LI Youjie, CHEN Zihan, DU Jiamei, CHEN Xiani, ZHANG Yue, YANG Qiyu, LI Zixuan, ZHAO Mingxing, ZHANG Xiancui, LI Peng, ZHOU Wei, ZHANG Yuliang, HU Lihua, WU Choufei. Effects of combined application of bio-organic fertilizer and microbial agents on soil carbon composition, enzyme activity, and carbon process in newly cultivated paddy fields[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(6): 1501-1505.

Figures/Tables 6

Table 1 Fertilization treatments

处理	667 m²基面肥/kg			667 m²穗肥/kg		667 m²穗肥/kg
处理	生物有机肥	菌剂	复合肥	菌剂	尿素	菌剂	尿素	氯化钾
1	750	10	17.5	10	14	10	3.5	1.4
2	750	15	17.5	10	14	10	3.5	1.4
3	750	15	17.5	15	14	10	3.5	1.4
4	750	20	17.5	20	14	10	3.5	1.4
5	750	25	17.5	25	14	10	3.5	1.4

Fig.1 Soil organic carbon fraction contents under different treatments

Table 2 Proportion of soil active organic carbon components to total organic carbon

处理	DOC/TOC/%	POC/TOC/%
1	0.26±0.15 a	57.98±8.88 a
2	0.30±0.14 a	44.95±14.70 a
3	0.41±0.36 a	71.19±52.86 a
4	0.17±0.06 a	44.61±21.62 a
5	0.05±0.01 a	24.31±2.50 a

Fig.2 Soil carbon cycling-related enzyme activity under different treatments

Fig.3 Soil carbon process under different treatments

Fig.4 Interrelationship between soil organic carbon components, carbon cycle enzyme activities and carbon processes

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