Study on the prevention and control of flutriafol and prothioconazole compound on wheat crown rot

doi:10.16178/j.issn.0528-9017.20240797

Abstract

Abstract:

Wheat crown rot caused by Fusarium pseudograminearum is an important plant disease in wheat production. At present, there is no highly resistant wheat variety that can effectively resist wheat crown rot, and chemical pesticides are mainly used for its prevention and control. Flutriafol and prothioconazole are widely used fungicides in the prevention and control of wheat diseases, and are widely praised for their excellent antibacterial effects. Clarifying the control effect of the flutriafol and prothioconazole compound on wheat crown rot is of great significance for effectively controlling wheat crown rot and prolonging the service life of fungicides. In this study, the Sun Yunpei co-toxicity coefficient method was used to evaluate the binary combination of flutriafol and prothioconazole and its antibacterial effect on Fusarium pseudograminearum. The results showed that the flutriafol and prothioconazole compound showed excellent antibacterial activity against F.pseudograminearum, and showed a synergistic effect within a certain mass ratio, and when the mass ratios were 1∶9 and 4∶6, the co-toxicity coefficient was the biggest in the mycelial growth and spore germination test of F.pseudograminearum, which showed synergistic effect. Meanwhile, pot experiments further showed that the control effect of the compound agent was higher than that of the single dose, and the difference was significant, when flutriafol and prothioconazole were compounded with 1∶9, it had excellent control effects on wheat crown rot, which were 86.53%, better than epoxiconazole, fluazinam, flutriafol, tebuconazole and prothioconazole with 65.21%, 42.28%, 52.38%, 31.54% and 63.64% control effects, respectively.

Key words: flutriafol, prothioconazole, fungicide mixture, pot experiment, wheat crown rot

CLC Number:

S482.2

LI Guanlong, ZHANG Sheng, LI Jianbing, QIAO Tiantian, TU Guangwei, TIAN Qizhen, LI Weiguo, LIU Runqiang, ZHOU Feng. Study on the prevention and control of flutriafol and prothioconazole compound on wheat crown rot[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(9): 2223-2229.

Figures/Tables 5

References 24

[1]	ALBRECHT T, WHITE S, LAYTON M, et al. Occurrence of wheat curl mite and mite-vectored viruses of wheat in Colorado and insights into the wheat virome[J]. Plant Disease, 2022, 106(10): 2678-2688.
[2]	郭子仪, 刘志金, 刘靓雯, 等. 浅谈小麦粉微生物的防控[J]. 粮食与食品工业, 2024, 31(4): 13-18.
[3]	KAZAN K, GARDINER D M. Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects[J]. Molecular Plant Pathology, 2018, 19(7): 1547-1562.
[4]	BACKHOUSE D, ABUBAKAR A A, BURGESS L W, et al. Survey of Fusarium species associated with crown rot of wheat and barley in eastern Australia[J]. Australasian Plant Pathology, 2004, 33(2): 255-261.
[5]	DENG Y Y, LI W, ZHANG P, et al. Fusarium pseudograminearum as an emerging pathogen of crown rot of wheat in Eastern China[J]. Plant Pathology, 2020, 69(2): 240-248.
[6]	XU F, YANG G Q, WANG J M, et al. Spatial distribution of root and crown rot fungi associated with winter wheat in the North China Plain and its relationship with climate variables[J]. Frontiers in Microbiology, 2018, 9: 1054.
[7]	MUDGE A M, DILL-MACKY R, DONG Y H, et al. A role for the mycotoxin deoxynivalenol in stem colonisation during crown rot disease of wheat caused by Fusarium graminearum and Fusarium pseudograminearum[J]. Physiological and Molecular Plant Pathology, 2006, 69(1/2/3): 73-85.
[8]	巫琪, 余曼丽, 曹立冬, 等. 中国小麦茎基腐病的农药防治策略与展望[J]. 现代农药, 2024, 23(4): 22-28.
[9]	徐飞, 李淑芳, 石瑞杰, 等. 黄淮麦区主栽小麦品种抗茎基腐病评价及茎秆和籽粒中毒素积累分析[J]. 植物病理学报, 2021, 51(6): 912-920.
[10]	陈宏州, 周晨, 庄义庆, 等. 江苏省水稻恶苗病菌种群鉴定及抗药性检测[J]. 植物保护, 2022, 48(2): 48-62.
[11]	吴佳文, 杨荣明, 杨红福, 等. 江苏省小麦赤霉病病菌对多菌灵抗药性发展及其治理对策研究[J]. 中国植保导刊, 2019, 39(12): 51-54, 78.
[12]	周锋, 韩奥辉, 崔伟伟, 等. 粉唑醇对假禾谷镰刀菌的抑菌活性及田间防效[J]. 贵州农业科学, 2024, 52(2): 64-69.
[13]	祁月月, 邵宇, 张平, 等. 丙硫菌唑和氟唑菌酰羟胺复配对小麦赤霉病菌的联合毒力[J]. 浙江农业科学, 2024, 65(3): 667-671.
[14]	张志伟, 魏秀华, 于海涛, 等. 丙硫菌唑·戊唑醇对小麦白粉病田间防效及其产量的影响[J]. 现代农业科技, 2023(17): 93-96.
[15]	佚名. Nortox在巴西推出新型杀菌剂Scudeiro(丙硫菌唑+戊唑醇)[J]. 农药, 2023, 62(12): 907.
[16]	陈宏州, 王兵兵, 王陈斌, 等. 丙硫菌唑与丙环唑混剂对小麦赤霉病菌的联合毒力及田间防效[J]. 中国农学通报, 2024, 40(2): 114-120.
[17]	中华人民共和国农业行业标准. 农药室内生物测定试验准则杀菌剂第2部分:抑制病原真菌菌丝生长试验平皿法: NY/T 1156.2—2006[S]. 北京: 中国农业出版社, 2006.
[18]	中华人民共和国农业行业标准. 农药室内生物测定试验准则杀菌剂第1部分:抑制病原真菌孢子萌发试验凹玻片法: NY/T 1156.1—2006[S]. 北京: 中国农业出版社, 2006.
[19]	SUN Y P, JOHNSON E R. Analysis of joint action of insecticides against house flies[J]. Journal of Economic Entomology, 1960, 53(5): 887-892.
[20]	ZHOU F, CUI Y X, ZHOU Y D, et al. Baseline pydiflumetofen sensitivity of Fusarium pseudograminearum isolates collected from Henan, China, and potential resistance mechanisms[J]. Plant Disease, 2023, 107(8): 2417-2423.
[21]	谭欢欢, 姜佳, 刘金亮, 等. 河南省禾谷镰孢菌对丙硫菌唑的敏感性[J]. 农药学学报, 2023, 25(2): 474-478.
[22]	王江来, 张锦锋, 马金秀, 等. 香芹酚和丁香酚对腐皮镰刀菌的抑菌活性及抑菌机理[J]. 微生物学通报, 2022, 49(5): 1638-1650.
[23]	李纳, 姚平平, 郑思, 等. 酷拉斯拌种对小麦茎基腐病的防治效果及产量影响[J]. 湖北植保, 2024(3): 28-30.
[24]	吴振锋, 王陆军, 任淑芳, 等. 苯醚甲环唑及其复配剂对小麦茎基腐病的防效[J]. 中国植保导刊, 2023, 43(9): 88-90.

杀菌剂处理	菌丝生长			孢子萌发
杀菌剂处理	EC₅₀/(μg·mL^-1)	R²	毒力回归方程	EC₅₀/(μg·mL^-1)	R²	毒力回归方程
戊唑醇	0.049	0.941	y=2.06+1.60x	0.077	0.838	y=3.18+2.68x
氟环唑	0.079	0.956	y=1.19+1.08x	0.226	0.899	y=0.58+0.89x
氟啶胺	0.139	0.962	y=0.95+1.13x	0.073	0.913	y=2.36+2.26x
粉唑醇	0.532	0.956	y=0.26+0.93x	0.338	0.932	y=0.57+1.19x
丙硫菌唑	0.602	0.963	y=0.18+0.87x	0.401	0.994	y=0.34+0.86x
1∶9	0.352	0.947	y=0.34+0.74x	0.209	0.965	y=0.70+1.02x
2∶8	0.744	0.981	y=0.12+0.97x	0.438	0.951	y=0.39+1.11x
3∶7	0.728	0.972	y=0.13+1.03x	0.605	0.986	y=0.22+1.00x
4∶6	0.192	0.970	y=0.55+0.76x	0.257	0.985	y=0.73+1.23x
5∶5	0.461	0.996	y=0.37+1.08x	0.502	0.952	y=0.31+1.05x
6∶4	0.259	0.973	y=0.51+0.86x	0.737	0.915	y=0.11+0.99x
7∶3	0.319	0.993	y=0.52+1.05x	0.449	0.906	y=0.36+1.01x
8∶2	0.328	0.994	y=0.50+1.04x	0.382	0.971	y=0.61+1.45x
9∶1	0.475	0.970	y=0.39+1.17x	0.416	0.955	y=0.35+0.93x

杀菌剂处理	菌丝生长			孢子萌发
杀菌剂处理	EC₅₀/(μg·mL^-1)	R²	毒力回归方程	EC₅₀/(μg·mL^-1)	R²	毒力回归方程
戊唑醇	0.049	0.941	y=2.06+1.60x	0.077	0.838	y=3.18+2.68x
氟环唑	0.079	0.956	y=1.19+1.08x	0.226	0.899	y=0.58+0.89x
氟啶胺	0.139	0.962	y=0.95+1.13x	0.073	0.913	y=2.36+2.26x
粉唑醇	0.532	0.956	y=0.26+0.93x	0.338	0.932	y=0.57+1.19x
丙硫菌唑	0.602	0.963	y=0.18+0.87x	0.401	0.994	y=0.34+0.86x
1∶9	0.352	0.947	y=0.34+0.74x	0.209	0.965	y=0.70+1.02x
2∶8	0.744	0.981	y=0.12+0.97x	0.438	0.951	y=0.39+1.11x
3∶7	0.728	0.972	y=0.13+1.03x	0.605	0.986	y=0.22+1.00x
4∶6	0.192	0.970	y=0.55+0.76x	0.257	0.985	y=0.73+1.23x
5∶5	0.461	0.996	y=0.37+1.08x	0.502	0.952	y=0.31+1.05x
6∶4	0.259	0.973	y=0.51+0.86x	0.737	0.915	y=0.11+0.99x
7∶3	0.319	0.993	y=0.52+1.05x	0.449	0.906	y=0.36+1.01x
8∶2	0.328	0.994	y=0.50+1.04x	0.382	0.971	y=0.61+1.45x
9∶1	0.475	0.970	y=0.39+1.17x	0.416	0.955	y=0.35+0.93x

杀菌剂处理	菌丝生长		孢子萌发
杀菌剂处理	EC₅₀/(μg·mL^-1)	CTC	EC₅₀/(μg·mL^-1)	CTC
戊唑醇	0.049	—	0.077	—
氟环唑	0.079	—	0.226	—
氟啶胺	0.139	—	0.073	—
粉唑醇	0.532	—	0.338	—
丙硫菌唑	0.602	—	0.401	—
1∶9	0.352	168.80	0.209	188.36
2∶8	0.744	78.84	0.438	88.26
3∶7	0.728	79.55	0.605	62.77
4∶6	0.192	297.86	0.257	145.21
5∶5	0.461	122.52	0.502	73.07
6∶4	0.259	215.43	0.737	44.90
7∶3	0.319	172.80	0.449	79.00
8∶2	0.328	166.06	0.382	91.35
9∶1	0.475	113.32	0.416	82.55

杀菌剂处理	菌丝生长		孢子萌发
杀菌剂处理	EC₅₀/(μg·mL^-1)	CTC	EC₅₀/(μg·mL^-1)	CTC
戊唑醇	0.049	—	0.077	—
氟环唑	0.079	—	0.226	—
氟啶胺	0.139	—	0.073	—
粉唑醇	0.532	—	0.338	—
丙硫菌唑	0.602	—	0.401	—
1∶9	0.352	168.80	0.209	188.36
2∶8	0.744	78.84	0.438	88.26
3∶7	0.728	79.55	0.605	62.77
4∶6	0.192	297.86	0.257	145.21
5∶5	0.461	122.52	0.502	73.07
6∶4	0.259	215.43	0.737	44.90
7∶3	0.319	172.80	0.449	79.00
8∶2	0.328	166.06	0.382	91.35
9∶1	0.475	113.32	0.416	82.55