Screening,identification and antagonistic mechanism for biological control of tea anthracnose

doi:10.16178/j.issn.0528-9017.20240224

Abstract

Abstract:

In order to obtain antagonistic bacteria for the biocontrol of tea anthracnose, 63 strains of bacteria were isolated and purified from the rhizosphere soil of tea plants by dilution plate method. Among these isolates, strain HZ-01 showed the highest inhibition rate(71.5%). Combined with morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence comparison analysis, strain HZ-01 was identified as Bacillus velezensis. Extracellular hydrolase activity analysis showed that strain HZ-01 could produce β-1,3-glucanase, protease and cellulase. The fermentation products had an obvious inhibitory effect on the mycelial growth and spore germination of Colletotrichum camelliae. The above results indicated that strain HZ-01 could be used as a candidate strain for biological control of tea anthracnose.

Key words: Colletotrichum camelliae, Bacillus, isolation and identification, biological control

CLC Number:

S435.71

LI Hongli, LU Jian, ZHAO Yun, HUANG Haitao, CUI Hongchun, ZHU Jianjie, REN Guohua. Screening,identification and antagonistic mechanism for biological control of tea anthracnose[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(8): 1902-1907.

Figures/Tables 6

Fig.1 Inhibitory effect of strain HZ-01 on Colletotrichum camelliae

Fig.2 Morphological observation of strain HZ-01

Table 1 Physiological and biochemical characteristics of strain HZ-01

测定项目	结果	测定项目	结果
V-P试验	-	D-甘露醇	-
柠檬酸盐	-	明胶液化	+
丙酸盐	-	淀粉水解	+
D-木糖	-	硝酸盐还原	+
L-阿拉伯糖	-	pH5.7生长	+
柠檬酸盐	-	耐盐7%	+
蔗糖	+	3%过氧化氢	+

Fig.3 Phylogenetic tree of strain HZ-01 based on the 16S rDNA sequences

Fig.4 Metabolites detection of strain HZ-01

Fig.5 Effect of strain HZ-01 on the growth of spore germination of Colletotrichum camelliae under microscope

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