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11 August 2024, Volume 65 Issue 8 Previous Issue    Next Issue

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Practices, challenges, and countermeasures for running a village SHI Guiqing, GAO Yajia, JIANG Weiqin, ZHANG Mingsheng 2024, 65 (8):  1745-1750.  DOI: 10.16178/j.issn.0528-9017.20240177 Abstract ( 142 )   HTML ( 8 )   PDF (1146KB) ( 312 )   The management of villages has become an epochal proposition of a beautiful countryside to the beautiful economic transformation. Village management is a practical way to develop new rural collective economy, activate rural resources, enrich rural business forms and promote the employment and income of villagers. This paper analyzes the origin, connotation, trend and significance of village management, focusing on the practice of village management in H village in northern Zhejiang Province participation of the whole people and joint efforts with farmers to achieve common prosperity. In the early stage, 31 projects were developed through village operations, brand building, camp development and business development, and a total of 100 000 visitors were received in 2023 to the H village scenic spot, producing better social and economic benefits. However, there are some problems such as poor operation mechanism, poor integration of resources, gradual decrease of flow and serious shortage of talents. In view of these challenges, the author puts forward some countermeasures and suggestions, such as clarifying the operation structure, attaching importance to brand marketing, integrating resources efficiently and strengthening the construction of talents. References | Related Articles | Metrics

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Current situation and development countermeasures of characteristic crops in Zhejiang Province CHEN Tao, SHEN Binliang, CAI Renxiang, ZHANG Weijiang 2024, 65 (8):  1751-1754.  DOI: 10.16178/j.issn.0528-9017.20240355 Abstract ( 164 )   HTML ( 8 )   PDF (1189KB) ( 410 )   Zhejiang has a wide variety of characteristic crops, with a perennial sowing area of 133 300 hm2 and a total yield of about 600 000 t. It is the main food and economic crop in mountainous and island areas, playing an important role in ensuring food security, increasing agricultural efficiency, and increasing farmers' income. With the improvement of people's living standards, the demand for characteristic crops has increased in quantity and price, and has developed into a sunrise industry. The development of characteristic crops towards fresh food and processing is a clear characteristic of the industry, characterized by high-quality, efficient, multi cropping cultivation, and miniaturization of fresh food. Promoting excellent varieties and practical technologies, implementing standardized production, and increasing the promotion and marketing of characteristic crop products are the future development directions. Figures and Tables | References | Related Articles | Metrics

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Effects of different cultivation modes on the yield and nutritional components of Abelmoschus sagittifolius ZHENG Bigu, LI Zhongyuan, ZHENG Guoming, WANG Hao, SHEN Bo, LU Yunfeng 2024, 65 (8):  1755-1758.  DOI: 10.16178/j.issn.0528-9017.20240183 Abstract ( 111 )   HTML ( 4 )   PDF (1152KB) ( 340 )   To study the effects of different cultivation modes on the yield and nutritional composition of Abelmoschus sagittifolius, and to screen out the suitable cultivation modes for promoting and applying Abelmoschus sagittifolius in Ningbo. The effects of three cultivation modes, including greenhouse, field, and under peach grove, on the key phenological stages, yield, and nutritional composition of Abelmoschus sagittifolius were investigated. The results showed that the first flowering period of Abelmoschus sagittifolius planted in greenhouse was earlier than that in field and under peach grove, the last flowering period was later, and the flowering period was the longest.The unit area and single plant yield of Abelmoschus sagittifolius were in the order of greenhouse, under peach grove, and field. The contents of protein, total saponins, amino acids and crude polysaccharides of Abelmoschus sagittifolius planted under peach grove were higher than those planted in field and greenhouse. Figures and Tables | References | Related Articles | Metrics

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Purification technology of total flavonoids from Tetrastigma hemsleyanum FU Changyong, XU Haishun 2024, 65 (8):  1759-1763.  DOI: 10.16178/j.issn.0528-9017.20240320 Abstract ( 100 )   HTML ( 3 )   PDF (2395KB) ( 326 )   In order to optimize the process conditions for the separation and purification of total flavonoids from polyamide, the content of total flavonoids in Tetrastigma hemsleyanum was determined by ultraviolet spectrophotometry, and the flavonoids were adsorbed by polyamide resin, and the static and dynamic adsorption and desorption properties were studied from three aspects: static and dynamic adsorption and desorption performance after different treatments. The results showed that the optimal conditions for the adsorption of total flavonoids in the leaves of Tetrastigma hemsleyanum were 15 mg·mL-1 and 1.75 g of polyamide in the loading solution, and the pH value was controlled at about 3.0 for static adsorption, and the highest adsorption rate was 76.7%. The polyamide resin was then desorbed with 60% ethanol with a desorption rate of up to 75.9%. Figures and Tables | References | Related Articles | Metrics

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HPLC fingerprint and content analysis of adenosine and uridine in Ganoderma lucidum LIN Ren'an, LI Jingjing, LIN Lin, HUANG Aiyun, GUAN Chuyun, ZHANG Haiying, ZHANG Yihan, LIN Ziqi, LIU Jingjing 2024, 65 (8):  1764-1769.  DOI: 10.16178/j.issn.0528-9017.20240435 Abstract ( 88 )   HTML ( 1 )   PDF (2210KB) ( 151 )   The fingerprint spectrum were used to detect and determine the content of adenosine and uridine in water-soluble components of Ganoderma lucidum,in order to find out the variation of the content of different spore powders and fruiting bodies. The results showed that there were significant differences in fingerprints between Ganoderma lucidum powder and spore powder from different sources; the content of uridine in the pileus of Ganoderma lucidum was 0.072-0.849 mg·g-1, the content of uridine in the stipe was 0.056-0.302 mg·g-1, and the content of uridine in the broken spore powder was 0.032-0.089 mg·g-1; the content of adenosine in the pileus of Ganoderma lucidum was 0.019-0.118 mg·g-1, the content of adenosine in the stipe was 0.005-0.024 mg·g-1, and the content of adenosine in the broken spore powder is 0.024-0.107 mg·g-1; There was no significant difference in the content of the main components of the unbroken spore powder harvested from the same variety but with different tides. There was no significant difference between the broken spore powder and the unbroken spore powder of the same variety. The difference of adenosine and uridine content in different batches of the same variety was obvious. The identification of broken spore powder can be achieved by using the fingerprint spectrum of high-performance liquid chromatography(HPLC)with only one peak in the area of 8.5-20.0 minutes for broken spore powder. The content of uridine and adenosine is used to identify whether the spore powder contains Ganoderma lucidum powder. Figures and Tables | References | Related Articles | Metrics

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Research and application of facilities, mechanization, and digital production technology for Chinese red bayberry TANG Xujun, WU Mengjiao, FENG Xingli, LI Shuangwei, XU Minjie, HAN Kaiyuan, HUA Shan 2024, 65 (8):  1770-1775.  DOI: 10.16178/j.issn.0528-9017.20240342 Abstract ( 117 )   HTML ( 2 )   PDF (11876KB) ( 291 )   This study aims to address the issues of low production efficiency, reliance on manual experience in agricultural management, and lack of quality and safety monitoring in traditional cultivation methods of Chinese red bayberry. By constructing a chemical, mechanized, and digital technology system, a systematic design and integration of an intelligent production system for Chinese red bayberry in mountain facilities was carried out, and an application demonstration was carried out at Tangyougui Family Farm in Lanxi City. A new model of facility based, mechanized, and digital production technology for Chinese red bayberry was summarized, aiming to build a scientific, efficient, and sustainable mountain bayberry cultivation technology system. The research results have important theoretical value and practical significance in promoting the transformation of traditional mountainous agriculture towards modernization, improving the quality and efficiency of agricultural products. Figures and Tables | References | Related Articles | Metrics

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Study on the effect of water-soluble fertilizer on the quality of early-maturing Citrus unshiu Marc. cv. Miyagawa Wase FENG Renfang, WU Shaohui, LAN Liuxin 2024, 65 (8):  1776-1780.  DOI: 10.16178/j.issn.0528-9017.20240162 Abstract ( 116 )   HTML ( 4 )   PDF (1476KB) ( 336 )   In this study, the effect of water-soluble fertilizer on the internal quality of early-maturing Citrus unshiu Marc. cv. Miyagawa Wase was studied by applying it at different growth stages. The results showed that spraying elemental water-soluble fertilizers on the leaves and applying organic water-soluble fertilizers on the ground could effectively improve the internal quality of citrus fruits, such as soluble solid content and sugar-acid ratio, among which the soluble solid content and sugar-acid ratio of the fruit treated with 1 000 times of organic water-soluble fertilizers were higher than those of other treatments and CK. At the maturity of October 19th, several water-soluble fertilizer treatments can effectively promote the fruit coloring, and the color difference values L, b* and ΔE* were significantly higher than those of CK, which was beneficial to the early market time. Therefore, the application of a large number of elements and organic water-soluble fertilizers in the growth period of early-maturing Citrus unshiu Marc. cv. Miyagawa Wase could effectively promote the coloring of peel and improve the internal quality of fruit. Figures and Tables | References | Related Articles | Metrics

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Preliminary report of six new strawberry varieties planted in greenhouses in Fenghua District, Ningbo City ZHANG Yubo, ZHANG Chengyi, LI Si, HE Yong, HE Yongchoulin, HU Meihua 2024, 65 (8):  1781-1785.  DOI: 10.16178/j.issn.0528-9017.20240401 Abstract ( 122 )   HTML ( 4 )   PDF (1149KB) ( 374 )   In order to enrich the variety types and optimize the variety structure of greenhouse strawberries in Fenghua, Ningbo, a comparative experiment was conducted on six new strawberry varieties, namely Yuexiu, Jiandehong, Fenyu 1, Mengjing, Danxue, and Baili, with the main locally planted varieties Hongyan and Zhangji as controls. The results showed that Yuexiu fruit was large, high-yielding, high-quality, and resistant to storage and transportation. Fenyu 1 and Danxue had good early ripening. Fenyu 1 was pink white in fruit surface color, Danxue was orange in fruit surface color with beautiful appearance and good quality. Baili was pure white in color, with a large fruit shape, a high sugar to acid ratio, and had good resistance to mites. Therefore, the four varieties of Yuexiu, Fenyu 1, Danxue, and Baili can be promoted and planted in Fenghua District. Figures and Tables | References | Related Articles | Metrics

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Breeding and cultivation techniques of a new middle and late maturing variety of Brassica rapa cv. Qingtai 305 HAN Guoqiang, ZHU Shouye, SHI Zhengkan, YANG Changqin, SHI Ruimin, ZHENG Ying, CHEN Yue, FAN Yi 2024, 65 (8):  1786-1790.  DOI: 10.16178/j.issn.0528-9017.20240128 Abstract ( 104 )   HTML ( 0 )   PDF (1157KB) ( 341 )   Qingtai 305 is a new middle and late maturing Brassica rapa variety, developed by Zheyan Agriculture (Hangzhou) Co., Ltd., using male sterile line 203 as the female parent and self incompatible line 205 as the male parent. The growth habit of the plant is upright, with strong growth momentum. The basal leaves are oval shaped and the leaves are oil green in color; The main stem is thicker, oily green, with a stem length of 18.6 cm and a single stem weight of 101.7 g. The stem is crispy and refreshing, delicate and sweet, and has good commercial value; Cabbage sprouts are neat, with strong lateral sprouting ability and a long harvesting time, making it suitable for planting in Zhejiang, Yunnan, Guangdong, Fujian, and Hubei Province. Figures and Tables | References | Related Articles | Metrics

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Identification and evaluation of Cucurbita moschata germplasm resources LEI Juanli, YUE Zhichen, CHEN Xiaoyang, LI Yan, TAO Peng, ZHAO Yanting, HU Qizan, LI Biyuan 2024, 65 (8):  1791-1801.  DOI: 10.16178/j.issn.0528-9017.20240375 Abstract ( 83 )   HTML ( 2 )   PDF (9273KB) ( 298 )   In order to better develop and utilize Cucurbita moschata germplasm resources, a two-year field planting identification on 248 Cucurbita moschata germplasm resources including 117 newly collected Cucurbita moschata local germplasm resources from the Zhejiang Project Group of the “Third National Census and Collection of Crop Germplasm Resources” was carried out. The agronomic traits, quality traits and disease resistance traits were investigated, analyzed and evaluated. The results showed that the Cucurbita moschata planting resources studied had rich diversity. The variation range of 13 agronomic traits was 12.30%-66.45%, among which the leaf width and leaf length had the lowest variation coefficients, which were 12.30% and 13.22%, respectively. The longitudinal diameter of commercial melon and the single weight of old melon had the highest variation coefficients, which were 66.45% and 53.70%, respectively. The variation range of 7 quality traits was 3.62%-81.82%, among which the minimum variation coefficient of water content was 3.62%, and the maximum variation coefficient of starch content was 81.82%. The powdery mildew index and virus disease index had the variation coefficients of 27.79% and 28.03%, respectively. Correlation analysis showed that the single weight of old melon was correlated with leaf width, first female flower node, main stem diameter, commercial melon thickness, commercial melon transverse diameter and water content to varying degrees. The water content was negatively correlated with soluble solids content, and the correlation coefficient was -0.919. The pectin content was significantly correlated with soluble sugar content, and the correlation coefficient was 0.801. Principal component analysis classified 22 traits into 7 principal components, with a cumulative contribution rate of 63.997%. The results of this study provide a theoretical basis for better development and utilization of Cucurbita moschata germplasm resources. Figures and Tables | References | Related Articles | Metrics

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Effects of different parental sowing period, planting density and fruiting node position on the seed yield of bottle gourd cultivars Zhepu 6 and Zhepu 9 LI Kunyu, WANG Jian, WANG Ying, LU Zhongfu, WU Xinyi, WANG Baogen, LI Guojing, WU Xiaohua 2024, 65 (8):  1802-1805.  DOI: 10.16178/j.issn.0528-9017.20230667 Abstract ( 91 )   HTML ( 2 )   PDF (1265KB) ( 331 )   In order to improve the seed yield of bottle gourd varieties (Zhepu 6 and Zhepu 9), the effects of different parent sowing dates, planting density and fruiting node position on seed yield were explored. The results showed that the female parent should not be sown later than the male parent during seed production of Zhepu 6, and the female parent should be planted close together, and the best distance between plants was 40 cm. The female parent of Zhepu 9 gourd should be sowed 10 days earlier than the male parent, and the planting density should be 40 cm, the fruiting node position should be a low node (7-8 nodes of the subvine). After implementing these cultural practices, the yield of seed production can be significantly improved. Figures and Tables | References | Related Articles | Metrics

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Effect of polyaspartic acid salts on reducing fertilizer utilization and increasing efficiency in potato planting FAN Zhanquan, LIU Shuanghu, ZHANG Kuo, LI Huashuai, JIAO Yongkang 2024, 65 (8):  1806-1809.  DOI: 10.16178/j.issn.0528-9017.20230628 Abstract ( 76 )   HTML ( 0 )   PDF (1135KB) ( 166 )   In order to improve the utilization rate of fertilizers, reduce the use of plant regulators, and improve the quality of potato tubers during potato cultivation, the study used a field experiment to study the weight loss and efficiency enhancement effect of applying a specialized polyaspartic acid salts fertilizer synergist during the planting process of potato, under the condition of reducing fertilizer application by 20%. The results showed that under the conditions of applying 15 kg·hm-2 of synergists and reducing fertilizer use by 20%, three specialized synergists increased potato yield by 5.86%-9.47%, and effectively improved the commercialization of potatoes. This indicates that specialized polyaspartic acid salts synergists can effectively reduce fertilizer use and increase potato yield characteristics, with T2 treatment being the most obvious. Meanwhile, the application of polyaspartic acid salts can effectively improve the quality of potato tubers. Figures and Tables | References | Related Articles | Metrics

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Effect of corn cob treatment on Pleurotus ostreatus production MA Haitao, PENG Dong, ZHAO Jianxuan, ZHU Hongcai, WANG Lingyan, DOU Shishu 2024, 65 (8):  1810-1813.  DOI: 10.16178/j.issn.0528-9017.20230643 Abstract ( 83 )   HTML ( 2 )   PDF (1147KB) ( 246 )   Corn cobs treated with clinker, fermentation material and fermentation with short-time high temperature were used for the cultivation of Pleurotus ostreatus, and the effects of the three treatments on the production of Pleurotus ostreatus were analyzed. The results showed that the mycelial growth rate under the fermentation with short-term high temperature was the fastest, reaching 0.72 cm·d-1, and there was no significant difference in the mycelial growth rate between clinker and fermentation material. In the early stage of mushroom emergence, the agronomic traits of the fruiting body of Pleurotus pleurotus produced by the three treatments were basically the same, and the agronomic traits of the fruiting body of Pleurotus pleurotus produced by the treatments of clinker and fermentation with short-term high temperature were better than those of fermentation material, and the biological efficiency of Pleurotus pleurotus was the highest under the corn cob treatment of clinker, and the lowest under the corn cob treatment of fermentation with short-time high temperature was the second, and the corn cob treatment of fermentation material was the lowest. Figures and Tables | References | Related Articles | Metrics

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Effects of different application amount of silicon fertilizer on growth and fruit quality of apple WEI Jiafeng, MA Xinyuan, ZHANG Ruiping 2024, 65 (8):  1814-1820.  DOI: 10.16178/j.issn.0528-9017.20230666 Abstract ( 116 )   HTML ( 3 )   PDF (1665KB) ( 354 )   In order to study the effects of different silicon fertilizers on the growth and fruit quality of apple, the nine-year-old Eai 2 was selected as the research object, and four treatments were set up, T1 (Rongdimei 15 mL per plant), T2 (Rongdimei 11.25 mL per plant), T3 (Rongdimei 7.5 mL per plant) and CK (no fertilization). The effects of different dosage of Rongdimei on the growth and fruit quality of apple were studied by measuring the growth and fruit quality of apple. The results showed that after the application of Rongdimei, the difference of T3 was the most significant (P<0.05). Compared with CK, the new shoot length, new shoot thickness, hundred-leaf weight and hundred-leaf thickness were increased by 17.41%, 13.51%, 17.52% and 11.68%, respectively. Fruit weight, hardness, increased by 12.96%, 15.20%, soluble solid content increased by 1.370 percentage points and titrable acid decreased by 0.048 percentage points;Leaf net photosynthetic rate, stomatal conductance, intercellular CO2 concentration and transpiration rate were increased by 10.84%, 8.08%, 5.25% and 22.66%, respectively. In conclusion, 7.5 mL per plant Rongdimei silicon fertilizer can effectively promote the growth and development of apple and improve fruit quality, and can be recommended for use in production. Figures and Tables | References | Related Articles | Metrics

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Effect of different composite substrate on growth and fruit quality of strawberry cultivar Benihoppe DI Huatao, LI Jinfeng, WAN Chunyan, CHEN Xueping, HUO Hengzhi, CHEN Bingyi 2024, 65 (8):  1821-1825.  DOI: 10.16178/j.issn.0528-9017.20230701 Abstract ( 97 )   HTML ( 2 )   PDF (1154KB) ( 369 )   To investigate the effects of four different composite substrates on the plant growth and fruit quality of strawberry cultivar Benihoppe, this study utilized common agricultural wastes such as mushroom residue, coconut bran and vinegar lees to mix with peat, perlite and vermiculite in a composite substrate, according to the demand of substrate characteristics for strawberry plant growth,then compared their effects on the plant growth and fruit quality of strawberry cultivar Benihoppe. These substrates were peat∶perlite∶vermiculite=60∶20∶20 (T1); coconut bran∶peat∶vermiculite∶perlite=50∶40∶5∶5 (T2); mushroom residue∶peat∶vermiculite∶perlite=40∶30∶15∶15 (T3); vinegar lees∶peat∶vermiculite∶perlite=60∶20∶10∶10 (T4). The results showed that there was no significant difference in plant morphology among T1, T2, and T3 treatment, while the leaf length, leaf width, and plant height of T4 treatment were significantly lower than T1 treatment; In terms of phenology, the fruit ripening period of T3 treatment was earlier than the other three treatments for 8-11 days; In terms of fruit yield and quality, T1 treatment had the highest single plant yield and soluble solid content, while T2 treatment had the highest average single fruit weight and lowest total acid content. The fruit hardness of T3 treatment was significantly lower than other treatments, but its soluble solid content was similar to T1, and significantly higher than T2 and T4. T4 treatment had the highest hardness and total acid content, but had the lowest single plant yield, single fruit weight, and soluble solid content. Therefore, coconut bran and mushroom residue can replace some peat, especially the T3 composite substrate has good application prospects in precocious cultivation. Figures and Tables | References | Related Articles | Metrics

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Differential analysis of soil factor affecting the formation of fruit quality in different Myric rubra varieties SUN Li, ZHENG Xiliang, LIANG Senmiao, ZHANG Qi, YU Zheping, QI Xingjiang, ZHANG Shuwen 2024, 65 (8):  1826-1832.  DOI: 10.16178/j.issn.0528-9017.20230737 Abstract ( 112 )   HTML ( 4 )   PDF (1220KB) ( 316 )   Using soil from 8 main production areas of Myric rubra in Zhejiang Province and two main varieties, Dongkui and Biqi, as experimental materials, correlation analysis was conducted between soil fertility and corresponding fruit quality indicators of different Myric rubra varieties. The main soil factors affecting the formation of fruit quality of different Myric rubra varieties were screened. Thirteen soil fertility factors and 18 fruit quality indicators were measured in the Dongkui and Biqi orchards, and correlation analysis was conducted. It was found that there were 94 significant correlations between soil fertility factors and fruit quality indicators in Biqi. Among them, soil organic carbon content, organic matter content, total nitrogen content were significantly positively correlated with fruit hardness, glucose content, vitamin C content, total flavonoids content, and total phenols content. Increasing the above indicators appropriately can improve fruit hardness, quality, and storage tolerance. There are 33 significant correlations between soil fertility factors and fruit quality indicators in Dongkui, among which available potassium content and total potassium content are significantly positively correlated with glucose content, vitamin C content, total flavonoids content, total phenols content, and total amino acids content. Increasing these indicators can appropriately improve fruit quality. The significant correlation between soil fertility and fruit quality in Dongkui was less than that of Biqi. Based on years of production experience, it is speculated that the formation of Dongkui fruit quality requires less soil fertility than Biqi, and requires more water, light, temperature, pruning and other factors than Biqi. Figures and Tables | References | Related Articles | Metrics

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Reflection and exploration on the high quality development of fresh grape industry in Changxing County ZHU Chunmei, MAO Yunlong, SHEN Binliang 2024, 65 (8):  1833-1837.  DOI: 10.16178/j.issn.0528-9017.20240354 Abstract ( 120 )   HTML ( 3 )   PDF (1284KB) ( 316 )   Fresh grapes are one of the leading industries in agriculture in Changxing County, Huzhou City, Zhejiang Province. It is also one of the main producing counties for fresh grapes in Zhejiang Province. Not only does it have a large planting area, high-quality products, and high comparative benefits, but it is also a “golden business card” for Changxing's external image promotion. The fresh grape industry has played a positive role in the adjustment of local planting structure, enhancing agricultural efficiency and increasing farmers' income. In recent years, with the increasing emphasis on grain production and the containment of the phenomenon of “non grainification” of cultivated land, a series of development problems have emerged, such as reduced planting area, uneven product quality, single planting varieties, and large annual price fluctuations. By analyzing the current situation of the grape industry in Changxing, summarizing its development trends, and deeply exploring the existing problems, this paper proposes green, high-quality, and efficient development strategies for the fresh grape industry in Changxing County, aiming to promote the high-quality development of the grape industry in Changxing. Figures and Tables | References | Related Articles | Metrics

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Study on the application effect of the main acidification management model for grape cultivated land in Changxing County WEN Wei, PAN Jianqing, YU Bo, XIE Jing, MA Junwei, YANG Yan, WANG Feng 2024, 65 (8):  1838-1842.  DOI: 10.16178/j.issn.0528-9017.20230428 Abstract ( 103 )   HTML ( 1 )   PDF (6374KB) ( 369 )   This study focuses on the acidification status of typical vineyard soils in Changxing County, Huzhou City, and compares the impacts of two primary methods “organic fertilizer + green manure” and “soil conditioner with humic acid + green manure” on soil acidification, nutrient quality, and grape quality, using conventional fertilization as the control. The results indicated that the “organic fertilizer + green manure (Vulpia myuros)” method had negligible effects on soil pH value, showing no significant influence on exchangeable H+ and total exchangeable acidity, but it significantly reduced the levels of exchangeable Al3+ and Mn. The second method, “soil conditioner with humic acid + green manure”, significantly lowered the pH value, exchangeable H+, exchangeable Al3+, total exchangeable acidity, and exchangeable Mn levels in the soil. Both methods significantly enhanced the soil's organic matter content, cation exchange capacity, and the levels of exchangeable calcium and magnesium, thereby increasing the soil's buffer capacity. These approaches also significantly boosted the grape fruit's soluble sugar, vitamin C, and total free amino acid content, with the “soil conditioner with humic acid + green manure” method showing a greater effect on vitamin C enhancement. Therefore, both primary methods effectively reduce soil acidity and enhance fertility, thereby improving grape quality. Figures and Tables | References | Related Articles | Metrics

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Selection of low-temperature-tolerant varieties of spray cut chrysanthemum and differences in the growth of tissue culture seedlings XU Zhihao, FU Manman, DING Xiaoyu, WU Chao, GUO Fangqi 2024, 65 (8):  1843-1850.  DOI: 10.16178/j.issn.0528-9017.20240421 Abstract ( 100 )   HTML ( 1 )   PDF (8556KB) ( 327 )   Using 28 spray cut chrysanthemum varieties as experimental materials, through the measurement and scoring of 15 agronomic traits, and assigning 64 points as the threshold value for variety selection, 18 cold-resistant excellent spray cut chrysanthemum varieties were screened. These varieties exhibited short or relatively short photoperiodic response cycles and growth periods. They were able to normally form and develop flower buds when exposed to the lowest daily temperature of 8 ℃ during the bud differentiation phase, and the quality of the cut flowers meet market demands. The research results can provide references for the selection of varieties for winter spray cut chrysanthemum facility cultivation and the breeding of tissue culture seedlings. Figures and Tables | References | Related Articles | Metrics

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Breeding and application of early rice variety Jinzao 645 with high yield, disease resistance, good quality and special purpose DENG Fei, WANG Kongjian, LU Aiguo, ZHOU Jianxia, BAO Yanhong, ZHU Hao 2024, 65 (8):  1851-1855.  DOI: 10.16178/j.issn.0528-9017.20240321 Abstract ( 161 )   HTML ( 2 )   PDF (1160KB) ( 393 )   Jinzao 645 is a high-yield, disease-resistant, good-quality and specialized conventional early indica rice variety, which was bred through the systematical selection of the cross of Jinzao 09 and Z73-34, and was bred by Jinhua Academy of Agricultural Sciences and Jinhua Sancai Seeds Company. It was approved by the Crop Variety Approval Committee of Zhejiang Province in 2021, and was recorded for introduction by the Department of Agriculture and Rurral Affairs of Jiangxi Province and was authorized as a new plant variety in 2023, and was listed as the leading variety of Zhejiang Province in 2024. Jinzao 645 showed the characteristics of tidy group, high tillering ability, resistance to rice blast, high lodging resistance, high yield, stable yield, and good adaptability when participated in the new variety regional test and demonstration planting in Zhejiang Province and Jiangxi Province from 2019 to 2023, and has high value of popularization and application. Figures and Tables | References | Related Articles | Metrics

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Comparison and analysis evaluation of rice varieties SHEN Fangqin, ZHANG Weijiang 2024, 65 (8):  1856-1859.  DOI: 10.16178/j.issn.0528-9017.20231237 Abstract ( 148 )   HTML ( 9 )   PDF (1114KB) ( 328 )   In order to accelerate the promotion and application of green and high-quality new varieties, based on the results of the 2022 Pinghu City rice new variety experiment, a comprehensive evaluation was conducted on the biological characteristics and field performance of each variety, and two potential varieties (Suxiangjing 100 and Xiushui 134) were selected, providing reference for the large-scale demonstration and promotion of excellent rice varieties in Pinghu. Figures and Tables | References | Related Articles | Metrics

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Effects of tea branch biochar on soil fertility and cadmium absorption and transport in rice LEI Chunsong, ZHANG Su'e, ZOU Wenhua, ZHONG Lijun, YE Zhengqian 2024, 65 (8):  1860-1866.  DOI: 10.16178/j.issn.0528-9017.20240344 Abstract ( 93 )   HTML ( 1 )   PDF (1414KB) ( 278 )   The exploration of contaminated cultivated land treatment technology is an important measure to ensure the rational utilization of cultivated land and alleviate the shortage of resources. In this study, tea branch biochar was used to conduct field experiments in cadmium-contaminated paddy fields by setting three different amounts (3 000, 6 000 and 9 000 kg·hm-2) to explore the effects of tea branch biochar on soil nutrients, available cadmium, rice growth and cadmium absorption and transport. The results showed that the 1000-grain weight, plant height and yield of medium dosage biochar treatment increased by 5.04%, 9.00% and 3.77%, respectively, compared with the blank control treatment, and the grain cadmium content of medium dosage biochar treatment decreased by 46.67%, which significantly reduced the biological concentration factor and translocation factor of cadmium in rice. At the same time, the soil pH value of medium dosage biochar treatment increased by 0.23, the contents of organic matter, alkali-hydrolyzable nitrogen, available phosphorus and available potassium of medium dosage biochar treatment increased by 11.41%, 13.77%, 8.28% and 40.94%, respectively, and the content of available cadmium in soil of medium dosage biochar treatment decreased by 13.04%. In summary, when the amount of tea branch biochar was 6 000 kg·hm-2, the goal of increasing rice yield and reducing cadmium, and simultaneously improving soil fertility could be achieved, which could be promoted and applied as a safe utilization technical measure. Figures and Tables | References | Related Articles | Metrics

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Effects of microbial compound fertilizer substitution of conventional chemical fertilizer and its reduced application on rice growth and soil fertility SHEN Jianguo, ZHENG Dongming, LOU Ling, WANG Jingwen, GU Wanfan, LI Agen 2024, 65 (8):  1867-1872.  DOI: 10.16178/j.issn.0528-9017.20230641 Abstract ( 106 )   HTML ( 2 )   PDF (1340KB) ( 324 )   Under the premise of reducing the amount of chemical fertilizer, the application effect of microbial compound fertilizer on rice was investigated with conventional fertilization as the control. The results showed that under the premise of reducing the total nutrients of chemical fertilizer by 20.0%, the application of microbial compound fertilizer had no effect on the growth and yield of rice. Under the premise that the total nutrients of chemical fertilizer were reduced by 30.0%, although there was a certain impact on the growth of rice plants, the impact on yield was small. Compared with conventional fertilization, the amount of chemical fertilizer nutrient input per unit yield of late rice with microbial compound fertilizer was lower, and the effect of chemical fertilizer reduction was obvious, and the effect of phosphorus reduction was the best. In addition, the application of microbial compound fertilizer can improve soil pH value, increase the activity of soil urease, protease and phosphatase, increase soil available nutrient content, and reduce the number of soil fungi. This study concluded that though the cost of rice fertilization increased after the application of microbial compound fertilizer, it could effectively improve soil quality and fertilizer utilization efficiency, and it was suggested that it should be popularized and applied as an effective measure to reduce the amount of rice fertilizer. Figures and Tables | References | Related Articles | Metrics

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Effects of zinc fertilizer spraying on rice growth and zinc content in rice under the condition of chemical fertilizer reduction LI Mengni, WU Lujie, YE Zhengqian 2024, 65 (8):  1873-1878.  DOI: 10.16178/j.issn.0528-9017.20230647 Abstract ( 98 )   HTML ( 2 )   PDF (1321KB) ( 333 )   The purpose of this study was to explore the effect of foliar zinc spraying on rice growth, yield and zinc content in rice under the condition of chemical fertilizer reduction. By setting up fertilizer reduction gradients of 5%, 10% and 20% in field experiments, zinc fertilizer was sprayed at the booting stage of rice. Experiments have shown that foliar zinc spraying can promote rice growth compared with conventional fertilization. Compared with conventional fertilization, the seed setting rate, 1000-grain weight and yield of rice increased by 3.64%, 4.37% and 8.17%, respectively, and the contents of nitrogen, zinc and phosphorus in rice increased by 25.33%, 43.14% and 11.14%, respectively, under the condition of 5% reduction of chemical fertilizer. In addition, with the decrease of chemical fertilizer use, the soil organic matter content increased, and the soil organic matter content increased by 9.91% when the chemical fertilizer was reduced by 20%. The application of foliar zinc fertilizer can improve rice yield, increase the level of zinc in rice, and obtain the maximum effect under the condition of 5% reduction of chemical fertilizer, which can be applied and promoted as a technical measure for chemical fertilizer reduction and efficiency increase. Figures and Tables | References | Related Articles | Metrics

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Characteristics, causes analysis, and prevention and control strategies of weed severe occurrence in rice fields in Jiaxing City in 2022 WANG Yeqing, CHEN Yiping, XU Weidong, CAO Mengjiao, YAO Zhangliang, LU Qiang 2024, 65 (8):  1879-1883.  DOI: 10.16178/j.issn.0528-9017.20230674 Abstract ( 107 )   HTML ( 5 )   PDF (1250KB) ( 328 )   Due to the abnormal high temperature and drought weather affecting weed control effectiveness, the direct seeding cultivation mode being conducive to weed growth, and the extensive field management being prone to grass or pesticide damage, the occurrence of weeds in the rice areas of the Zhejiang North Plain in 2022 is severe, with multiple types of occurrence and poor control effects, and grass damage is significantly more severe than usual. This article takes Jiaxing City as an example to analyze the characteristics and reasons for the severe occurrence of weeds in rice fields in Jiaxing area in 2022, and proposes countermeasures and suggestions for the next comprehensive management of weeds in rice fields: Firstly, we need to improve farmland infrastructure, promote the construction of high standard farmland, and enhance disaster prevention and reduction capabilities; Secondly, we must vigorously promote rice machine transplanting and switch from live broadcasting to transplanting to reduce the occurrence of weeds; Thirdly, we need to improve weed control technologies, vigorously promote them, and increase their coverage. Figures and Tables | References | Related Articles | Metrics

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Occurrence and control of Phyllotreta striolata (Fabricius) in Shenzhen CHEN Xiaoqin, PENG Liya, YANG Jiaoxu, LU Yong, YIN Xiangyu, ZHANG Chen 2024, 65 (8):  1884-1887.  DOI: 10.16178/j.issn.0528-9017.20221303 Abstract ( 99 )   HTML ( 1 )   PDF (1766KB) ( 199 )   The occurrence of Phyllotreta striolata Fabricius were investigated in the vegetable planting base of Bao'an, Guangming and Longhua District in Shenzhen. The results showed that, the occurrence dynamic of Phyllotreta striolata(Fabricius) showed an obvious bimodal type. One peak was from October to December 2020, the average damage rate of host plant ranged from 40.4% to 50.0% and the average population density ranged from 82.7 to 121.9 adults per 100 plants. The other peak was from February to May 2021, the average damage rate of host plant ranged from 55.5% to 70.0% and the average population density ranged from 90.2 to 139.8 adults per 100 plants. The average damage rate and the population density of 100 adults per 100 plants of different host plants from high to low were as follows: Raphanus sativus>Brassica rapa ssp. pekinensis>Brassica juncea>Brassica rapa var. parachinensis. Cucumis sativus, Momordica charantia, Luffa cylindrica, Vigna unguiculata, Phaseolus vulgaris, Lactuca sativa were not found to be damaged. The number of adults dynamic showed an obvious bimodal type with yellow sticky trap method, one peak was in December 2020, the other peak was in February 2021. In August, September 2020 and June to July 2021, fewer adults were trapped. Figures and Tables | References | Related Articles | Metrics

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Reduction of pesticide and enhancement of prevention and control of Sclerotinia sclerotiorium(Lib.)de Bary on rape in Tongxiang SHEN Yuyuan, YAO Zhangliang, ZHANG Qianqian, YANG Fengfan, JIN Xinmei 2024, 65 (8):  1888-1891.  DOI: 10.16178/j.issn.0528-9017.20230576 Abstract ( 105 )   HTML ( 0 )   PDF (1224KB) ( 282 )   This article studies through the fungicide tests in rape fields under the two cultivating ways of direct seeding and transplant, in order to find out the control of Sclerotinia sclerotiorium(Lib.)de Bary on rape in different farming methods in Tongxiang,north of Zhejiang Province.It shows that Sclerotinia sclerotiorium(Lib.)de Bary occurs more severely in direct seeding plot than in transplant plot.Both procymidone and carbendazim bring out the different control effect under the different cultivating ways,the effect of procymidone is better than that of carbendazim in both ways.Meanwhile,the application amount of procymidone per 667 m2 is one fifth of carbendazim, and the reduction of fungicide use per unit area is obvious.Procymidone can be used to control Sclerotinia sclerotiorium(Lib.)de Bary on rape instead of carbendazim,it could reduce the amount of fungicide use and improve the efficiency for reference. Figures and Tables | References | Related Articles | Metrics

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Effect of 10% tripyrasulfone·anilofos in the control of weeds in rice fields WU Tongxing 2024, 65 (8):  1892-1895.  DOI: 10.16178/j.issn.0528-9017.20230928 Abstract ( 120 )   HTML ( 3 )   PDF (1268KB) ( 285 )   In response to the problems of large populations, rapid variation, high resistance, and difficult control of weeds in rice fields, an experiment was conducted on the weed control effect of tripyrasulfone·anilofos herbicide on transplanted rice and direct seeding rice fields. The experimental results indicate that, whether in transplanting rice fields or direct seeding rice fields, the application amount of 10% tripyrasulfone·anilofos dispersible oil suspension (OD) active ingredient is 375-600 g·hm-2 after the transplanted rice or the direct seeded rice returns to green, weeds such as barnyard grass are treated by spraying at 2-3 leaf stages. It has good control effect on weeds such as barnyard grass and millet in rice fields. The control effect of the main weeds in the field is 97.4% or above, and the rice yield increases by 42.10% or above, which is safe for rice production. Therefore, 10% tripyrasulfone·anilofos OD can be used to control weeds such as barnyard grass and millet in rice fields. Figures and Tables | References | Related Articles | Metrics

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Control effect of the combination of seed dressing with chlorbenzamide and stem leaf spray with spinetoram on Spodoptera frugiperda of maize ZHANG Yu, WANG Fengliang, ZHANG Haibo, BIAN Kangya, JIANG Chunyi, CHEN Yongming, ZHANG Fang, ZHU Feng, YANG Rongming 2024, 65 (8):  1896-1901.  DOI: 10.16178/j.issn.0528-9017.20230696 Abstract ( 94 )   HTML ( 1 )   PDF (1188KB) ( 242 )   Since the invasion of Spodoptera frugiperda into China, its occurrence has become more and more extensive, and its harm has become more and more serious. Although there are a lot of reports on the control of Spodoptera frugiperda in China, there are few studies on the control of Spodoptera frugiperda by the combination of seed dressing agent and stem leaf spray. For this reason, in 2022, the author carried out the safety, field control effect and yield increase effect tests of four treatments on maize, namely, 50% chlormebenzamide seed dressing and 25% spinetoram stem and leaf spray, 50% chlormebenzamide seed dressing and 5.7% emamectin benzoate stem and leaf spray, 25% spinetoram stem and leaf spray, and 5.7% emamectin benzoate stem and leaf spray. The results showed that the combination of chloramphenicol seed dressing+spinetoram stem and leaf spray had significant effects on seedling protection, insect control and yield increase. The emergence rate of maize after chemical seed mixing treatment was significantly higher than that of the blank control, and the seed mixing agent had a promoting effect on the emergence of maize; In the case of a large number of Spodoptera frugiperda, seed dressing with 50% chloramphetamine can effectively control the damage of Spodoptera frugiperda to maize in the early growth stage, but in the middle and late growth stage of maize, it still needs to rely on efficient agents such as spinetoram for stem and leaf spray to control the damage of Spodoptera frugiperda. Each chemical treatment has a significant effect on the yield increase of maize, and its trend is that the combination of 50% chloramphenicol seed dressing and 25% spinetoram stem and leaf spray>the combination of 50% chloramphenicol seed dressing and 5.7% emamectin benzoate stem and leaf spray>the combination of 25% spinetoram stem and leaf spray>5.7% emamectin benzoate stem and leaf spray. Figures and Tables | References | Related Articles | Metrics

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Screening,identification and antagonistic mechanism for biological control of tea anthracnose LI Hongli, LU Jian, ZHAO Yun, HUANG Haitao, CUI Hongchun, ZHU Jianjie, REN Guohua 2024, 65 (8):  1902-1907.  DOI: 10.16178/j.issn.0528-9017.20240224 Abstract ( 101 )   HTML ( 3 )   PDF (3159KB) ( 241 )   In order to obtain antagonistic bacteria for the biocontrol of tea anthracnose, 63 strains of bacteria were isolated and purified from the rhizosphere soil of tea plants by dilution plate method. Among these isolates, strain HZ-01 showed the highest inhibition rate(71.5%). Combined with morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence comparison analysis, strain HZ-01 was identified as Bacillus velezensis. Extracellular hydrolase activity analysis showed that strain HZ-01 could produce β-1,3-glucanase, protease and cellulase. The fermentation products had an obvious inhibitory effect on the mycelial growth and spore germination of Colletotrichum camelliae. The above results indicated that strain HZ-01 could be used as a candidate strain for biological control of tea anthracnose. Figures and Tables | References | Related Articles | Metrics

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Observation on morphological characteristics and oviposition behavior of Cylas formicarius LI Jianzhong, TU Yuwen, LIU Min, ZHENG Hongze, ZHANG Jinming 2024, 65 (8):  1908-1910.  DOI: 10.16178/j.issn.0528-9017.20240356 Abstract ( 114 )   HTML ( 5 )   PDF (5588KB) ( 154 )   Cylas formicarius is an important quarantine pest during the production and storage of sweet potato. The adults eat root tubers, tender buds and tender stems, and the larvae eat sweet potatoes and their thick vines. The damaged sweet potatoes have a fishy smell and can not be eaten. The feeding of Cylas formicarius can cause other diseases, such as soft rot, and cause serious harm to the production of sweet potatoes. In this paper, the morphology and oviposition behavior of Cylas formicarius were photographed and described to provide identification basis, which is beneficial for improving the quarantine, monitoring and pest control abilities of technicians. Figures and Tables | References | Related Articles | Metrics

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Ginger extract test paper for the preservation of fresh figs LIU Li, XU Weiwei, QIAN Jichang, JIN Liang, LI Xiaobai 2024, 65 (8):  1911-1914.  DOI: 10.16178/j.issn.0528-9017.20230636 Abstract ( 82 )   HTML ( 1 )   PDF (1141KB) ( 208 )   The preservation test paper made of ginger extract has a preservation effect on fresh figs. 75% ethanol extract of ginger (final concentration of 1 g·L-1) was evenly sprayed on food-grade filter paper and the paper was dried to make bio-preservation test paper, which are used to cover the upper and lower layers of the packaging box, place the figs in it and observe its preservation effect. The results showed that compared with the control, the biopreservation test strips significantly reduced the weight loss rate and decay rate of fruits, and maintained higher contents of soluble solids and VC. In general, the ginger preservation test paper extended the storage period of figs by 2-4 days. The quality of fresh figs is maintained, and it can be used for long-distance storage and transportation of figs. Figures and Tables | References | Related Articles | Metrics

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Effect of drying process on volatile compounds of Lentinula edodes WU Jiali, LIU Shu, WANG Zhe, WU Shulian, PENG Guoping 2024, 65 (8):  1915-1919.  DOI: 10.16178/j.issn.0528-9017.20240210 Abstract ( 83 )   HTML ( 0 )   PDF (2874KB) ( 169 )   Volatile compounds play an important role in the aroma of Lentinula edodes. In this study, headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was utilized to investigate the volatile compounds in Lentinula edodes subjected to different drying methods: freeze drying (FD), low temperature drying (LD), high temperature drying (HD), and infrared drying (ID). A total of 48 volatile compounds were identified across the four processes. Comparative analysis of these compounds revealed higher levels of total volatile compounds, aldehydes, and sulfur compounds in the HD and ID processes compared with the LD and FD processes. Specifically, the contents of dimethyl trisulfide, 1,2,4-trithiopentane, and lenthionine were found to be significantly elevated in the HD and ID processes. These findings suggest that Lentinula edodes processed using HD and ID methods exhibit better quality in terms of aroma compounds. This study can provide a theoretical basis for the research of Lentinula edodes processing technology. Figures and Tables | References | Related Articles | Metrics

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Analysis of residual results of 8 pesticides in Allium tuberosum Rottler ex Sprengle SUN Meimei 2024, 65 (8):  1920-1922.  DOI: 10.16178/j.issn.0528-9017.20240326 Abstract ( 113 )   HTML ( 2 )   PDF (1141KB) ( 332 )   In order to evaluate the safety of eight pesticides, including procymidone, chlorpyrifos, eta-cyfluthrin, cypermethrin, iprodione, acetamiprid, phoxim and avermectin, on Allium tuberosum Rottler ex Sprengle, the recommended dosage of the eight pesticides and the actual dosage used by farmers were applied. Samples from different intervals were collected for pesticide residue analysis. The experimental results showed that the detection values of chlorpyrifos were 0.100 0 and 0.200 0 mg·kg-1, respectively, until 30 days after treatment, still exceeding the maximum limit of 0.02 mg·kg-1. The conventional treatment A of procymidone degraded to 4.450 mg·kg-1 until 7 days after treatment, meeting the limit requirements; Treatment B did not degrade to 3.540 0 mg·kg-1 until 21 days after treatment, meeting the limit requirements. The conventional treatment A of the other six pesticides all degraded to the maximum limit within 7 days after treatment, among which the residual levels of eta-cyfluthrin, iprodione, and acetamiprid meet the limit requirements after 2 hours of treatment, and can be used with confidence; The residual levels of cypermethrin and avermectin after 3 days of treatment and phoxim after 7 days of treatment meet the limit requirements. The residual amount of cypermethrin in treatment B, which is 1.2 times of the conventional dosage, is delayed until 10 days after treatment to meet the limit requirements. Figures and Tables | References | Related Articles | Metrics

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Simultaneous detection of chlorothalonil and cyproconazole by high-performance liquid chromatography GONG Guohua 2024, 65 (8):  1923-1928.  DOI: 10.16178/j.issn.0528-9017.20230849 Abstract ( 84 )   HTML ( 1 )   PDF (4493KB) ( 156 )   Chlorothalonil and cyproconazole are both efficient fungicides, but there is currently no analytical method for simultaneously detecting these two components. This study aims to establish an analytical method that can simultaneously detect chlorothalonil and cyproconazole. A high-performance liquid chromatography (HPLC) was used to configure an ODS-3 C18 column and a UV detector. Methanol and water were used as mobile phases, and chlorothalonil and cyproconazole were separated and quantitatively analyzed in a 40.5% chlorothalonil cyproconazole suspension at a wavelength of 224 nm. The results showed that the linear correlation coefficients of this method for chlorothalonil and cyproconazole were 0.999 7 and 0.999 9, respectively, with standard deviations of 0.132 0 and 0.013 7, coefficient of variation of 0.35% and 0.45%, and average recovery rates of 99.34% and 99.62%, respectively. This method has good precision and accuracy, and can simultaneously detect chlorothalonil and cyproconazole in 40.5% suspension of chlorothalonil and cyproconazole, and is suitable for the detection of related products. Figures and Tables | References | Related Articles | Metrics

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Study on the influence mechanism of the acceptance degree of large-scale pig breeding support policy under the impact of major epidemic YAO Wenjie 2024, 65 (8):  1929-1938.  DOI: 10.16178/j.issn.0528-9017.20221292 Abstract ( 92 )   HTML ( 1 )   PDF (1736KB) ( 280 )   Combined with the realistic background of the impact of African swine fever on China's pig breeding industry, this paper uses the large sample sub-regional sampling survey data nationwide, starts from the two attributes of policy tools and intervention objects, and makes necessary considerations on the rationality of support policies for large-scale pig breeding through the ex-ante attitudes of large-scale farmers. The results showed that after the outbreak of a major epidemic, the large-scale farmers were willing to accept the support policy before the actual support policy, and the large-scale farmers who were willing to accept the support policy actually received the support policy, then the large-scale pig breeding support policy was reasonable. For large-scale pig farmers, they are willing to accept direct support policies if they think that the previous large-scale pig breeding support policies are more transparent or fair. It is believed that the previous large-scale pig breeding support policy is more transparent, and the government will agree with the market-oriented action of the pig breeding industry. For medium-scale farmers, they are willing to accept direct support policies if they agree with the government's planning of pig breeding industry. It is believed that the previous large-scale pig breeding support policies are more transparent, and by strengthening the recognition of the government's planning actions for the pig breeding industry, they are reluctant to accept direct support policies. Figures and Tables | References | Related Articles | Metrics

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Isolation, virulence gene identification, and antimicrobial resistance analysis of hybrid enterotoxigenic and Shiga toxin-producing Escherichia coli (ETEC/STEC) strain from pigs JIANG Liming, CHEN Yijie, YUAN Xiufang, LI Junxing 2024, 65 (8):  1939-1943.  DOI: 10.16178/j.issn.0528-9017.20230291 Abstract ( 98 )   HTML ( 2 )   PDF (3575KB) ( 295 )   Post weaning diarrhea (PWD) in piglets is usually caused by infection with enterotoxigenic Escherichia coli (ETEC), which typically carries one or more of the enterotoxins LT, STa, and STb. Pigt edema disease (ED), also known as bowel edema or gut edema, is an acute and lethal infectious disease caused by infection with Shiga toxin-producing Escherichia coli (STEC), with a high mortality rate. This bacterium usually carries toxins such as Stx1 and Stx2. In this study, we diagnosed a case of PWD complicated by ED with high mortality in piglets, and isolated and identified a hybrid strain carrying both enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) virulence genes. It shows that the hybrid strain carried the F18 adhesin gene, enterotoxin genes (STa, STb, LT), and edema disease causing toxin gene (Stx2e). The results of antimicrobial sensitivity test showed that the E. coli isolate was sensitive to five drugs, including cefotaxime and amikacin, moderately sensitive to two drugs, including spectinomycin and ciprofloxacin, and resistant to 14 drugs, including enrofloxacin and streptomycin. The isolate is a hybrid Escherichia coli (ETEC/STEC), and shows strong resistance to multiple antibiotics. Figures and Tables | References | Related Articles | Metrics

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Evaluation of inactivation effect of commercial disinfectant on African swine fever virus based on model virus NI Zheng, ZHU Yinchu, SUN Bingbing, YUN Tao, CHEN Liu, XU Hui, YE Weicheng, HUA Jionggang, ZHANG Cun 2024, 65 (8):  1944-1949.  DOI: 10.16178/j.issn.0528-9017.20230621 Abstract ( 84 )   HTML ( 1 )   PDF (5872KB) ( 270 )   In order to evaluate and prove the elimination effect of commercial disinfectants on African swine fever virus (ASFV), and to provide reference for the screening of disinfectants. In this study, the similarity of physicochemical characteristics of pseudorabies virus (PRV) and ASFV virus was used to establish a disinfectant cell evaluation model with PRVas an alternative marker strain. The inactivation effect of three commercially available disinfectants on PRV grown in HBK-21 cells was tested in vitro. The disinfecting effect of the three disinfectants was evaluated from several aspects on the antiviral effect of the three disinfectants. The results showed that glutaraldehyde methyl bromide solution with an effective content of 0.125 g·L-1, peracetic acid solution with an effective content of 0.5 g·L-1 and sodium dichloroisocyanurate could effectively inactivate PRV at room temperature for 30 min. Among them, peracetic acid comprehensive evaluation is the best; Glutaraldehyde decyl methyl bromide is the most cost-effective, but it requires a long time of elimination and is seriously affected by low temperature. This study can provide theoretical basis for disinfectant screening for the prevention and control of ASFV infection in livestock farms, and provide reference for the establishment of disinfection standards for ASFV. Figures and Tables | References | Related Articles | Metrics

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Application of immobilized microorganism technology on soil remediation of farmland in Xiaoqinling gold mining area LI Lijun, ZHONG Wei, LI Tao 2024, 65 (8):  1950-1956.  DOI: 10.16178/j.issn.0528-9017.20230614 Abstract ( 90 )   HTML ( 3 )   PDF (1740KB) ( 307 )   In order to explore new methods of soil pollution prevention, the application of immobilized microorganism technology in soil ecological remediation was studied. The heavy metal contents in the soil of Xiaoqinling gold mining area and the surrounding farmland in Western Henan Province were determined and evaluated, it was found that the risk of Pb2+ element pollution in the soil in this area was high. The mixed bacteria of Bacillus laterosporus, actinomycetes and yeast were immobilized to carry out soil bioremediation in situ, the results showed that the urease activities of soil treated with immobilized bacteria were significantly higher than that of CK. In order to improve the passivation efficiency and removal rate of Pb2+, the single factor test and response surface methodology were used to analyze the factors affecting the soil remediation effect, the optimal removal conditions of Pb2+ were determined finally: the dosage of immobilized mixed bacteria was 10.496%, the treatment temperature was 30.784 ℃, the pH value was 6.242, and the theoretical removal rate of Pb2+ was 72.754%. The model was verified by experiment, the result of experiment was close to the theoretical value, indicating that response surface analysis can optimize the soil remediation experimental parameters. Figures and Tables | References | Related Articles | Metrics

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Selection of summer green manure varieties and winter wheat rotation in southern Henan DING Li, ZHANG Lin, DU Guanghui, ZHANG Lixia, SHI Pengfei, NIE Liangpeng, LING Jingwei, PAN Ziliang, LYU Yuhu 2024, 65 (8):  1957-1962.  DOI: 10.16178/j.issn.0528-9017.20231157 Abstract ( 91 )   HTML ( 2 )   PDF (1172KB) ( 341 )   Six summer green manure varieties were planted in Xinyang City, and inoculation and non-inoculation of rhizobia were set up, and a total of 12 treatments were set up for planting experiments. Summer green manure varieties suitable for fertilizer reduction of winter wheat were selected by planting and screening to prepare for the utilization. The green manure varieties were comprehensively evaluated from various aspects. The results showed that there was little difference in the emergence stage of the 12 treatments, with Jufeng and Fendou Mulv 2 budding stage being the earliest, and Tianjing budding stage being the latest. Fendou Mulv 2 had the earliest flowering period, and Tianjing had the latest flowering period. Fendou Mulv 2 has the earliest full flowering period, and Tianjing has the latest full flowering period, but both can be used for green manuring and water-logged compost at the end of August. There was a large difference in the yield of fresh grass at the peak flowering stage, and the fresh yield of Tianjing inoculated with rhizobia was the highest, and the nutrient content of nitrogen, phosphorus and potassium in Jufeng and Zheng 1307 plants was the best. The nutrient accumulation of nitrogen, phosphorus and potassium was the highest in Tianjing inoculated with rhizobia. Inoculated with rhizobia can reduce soil temperature and increase soil moisture. Increasing soil stability and wind erosion resistance can improve soil fertility. The preferred variety of summer green manure in rotation with winter wheat was preliminarily screened out as Tianjing (inoculated with rhizobia). Figures and Tables | References | Related Articles | Metrics

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Comparison and cooperation between China and Pakistan in the background of the Belt and Road Initiative: a case study of Zhejiang Province WANG Jie, ZHU Youwei, QIN Yebo, DENG Meihua, LI Mengqi 2024, 65 (8):  1963-1967.  DOI: 10.16178/j.issn.0528-9017.20240386 Abstract ( 105 )   HTML ( 1 )   PDF (1260KB) ( 335 )   Since the implementation of the “the Belt and Road Initiative”, Pakistan and China have been increasingly close in cooperation and pay more attention to the agricultural field. As one of the leading provinces in China's agricultural modernization, Zhejiang Province's mature management mechanism and advanced agricultural technology contribute to further promoting pragmatic agricultural cooperation between China and Pakistan. On the basis of field research and literature analysis, this paper compares the overall development of the planting industry in Zhejiang Province and Pakistan, focusing on the planting situation and development trend of wheat, corn and rice, the three major crops of the two sides, explores the differences in the planting industry between the two sides, and deeply analyzes the potential and shortcomings of Pakistan's planting industry in terms of resources, technology, equipment, mechanisms, etc. Finally, it puts forward suggestions and prospects to promote the cooperation and development of the planting industry between the two sides, such as expanding the field and depth of cooperation, increasing the international cultivation of agricultural leading enterprises, and strengthening the policy and fund guarantee for long-term cooperation, so as to provide a reference for promoting the construction of the “the Belt and Road”. Figures and Tables | References | Related Articles | Metrics

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Can fiscal and financial synergy ensure food security-Empirical evidence based on provincial panel data LI Zongyi, GAO Yajia, CHEN Yaoyao 2024, 65 (8):  1968-1976.  DOI: 10.16178/j.issn.0528-9017.20240220 Abstract ( 97 )   HTML ( 0 )   PDF (1213KB) ( 166 )   Based on panel data at the provincial level from 2009 to 2020, this study empirically tests the impact of fiscal and financial synergy on grain production using a panel bidirectional fixed effects model. Research has shown that fiscal and financial synergy can significantly promote food production, and the results still hold true even after addressing endogeneity issues, replacing dependent variables, lagging explanatory variables by one period, and excluding directly administered municipality. The threshold analysis results show that differences in total factor productivity lead to a threshold effect on the impact of fiscal and financial synergy on grain production. In regions with high total factor productivity, fiscal and financial synergy has a greater promoting effect on grain production. Heterogeneity analysis shows that compared with the eastern and non grain producing regions, the fiscal and financial synergistic effects of the western and northeastern regions, as well as the grain producing regions, are greater. Therefore, in steadily advancing the food security strategy, attention should be paid to the synergistic effect of fiscal and financial policies. Figures and Tables | References | Related Articles | Metrics

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Research on leisure agriculture production behavior of Zhejiang farmers based on IAD extended decision model ZHANG Anmin 2024, 65 (8):  1977-1984.  DOI: 10.16178/j.issn.0528-9017.20230525 Abstract ( 96 )   HTML ( 1 )   PDF (1205KB) ( 298 )   Based on the institutional analysis and development framework (IAD) extended decision-making model, this study utilized survey data from 2 968 households in Zhejiang Province to analyze the impact of household characteristics, family conditions, external environment and market services, business rules, and reform cognition on the production behavior of leisure agriculture among farmers. The research showed that external environment and market services, business rules, reform awareness, and household head characteristics were significant factors affecting whether farmers engage in leisure agriculture production. Further analysis revealed that there were significant differences in the influencing factors between early and late engagement of farmers in leisure agriculture production; Early adopters were more concerned about the freedom of decision-making and income expectations in leisure agriculture production, while late adopters were more concerned about the completeness of leisure agriculture facilities and the convenience of obtaining subsidies. Therefore, differentiated guidance measures should be implemented according to time and place to promote the rationalization and high-quality development of leisure agriculture. Figures and Tables | References | Related Articles | Metrics

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Research on development strategy of Camellia oleifera industry in Guangxi township supported by technology based on SWOT-AHP WU Guowen, TANG Qing, TAN Guifei, TAN Siyi, LI Zhouli 2024, 65 (8):  1985-1990.  DOI: 10.16178/j.issn.0528-9017.20231196 Abstract ( 95 )   HTML ( 0 )   PDF (1207KB) ( 309 )   In order to comprehensively analyze the factors that affect the development of the Camellia oleifera industry supported by science and technology in Guangxi townships, and then formulate a reasonable development strategy, this paper first constructs an analysis model of the advantages, disadvantages, opportunities and challenges of the development of the Camellia oleifera industry supported by science and technology in Guangxi townships, and then makes a quantitative analysis of each factor to position its development strategy. The research shows that science and technology to support the development of Camellia oleifera industry in Guangxi township should be based on the SO strategy, that is, should give full play to the advantages of Camellia oleifera research foundation, scientific research results, perfect scientific research team, good mass base, firmly grasp the national and autonomous region's attention to Camellia oleifera industry development and market demand opportunities, constantly make up for the development of disadvantages, calmly respond to external challenges. On this basis, some suggestions were put forward to optimize the technical support of Camellia oleifera in township, including optimizing and perfecting the policy system of Camellia oleifera science and technology promotion, increase investment in technology promotion of Camellia oleifera; Training highly professional science and technology extension personnel, improve farmers' awareness of science and technology. Figures and Tables | References | Related Articles | Metrics

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Exploration and practice of building a digital service system for agriculture, rural areas and farmers in the new era under the background of building a strong agricultural country: a case study of Quzhou City ZHANG Xin, XU Bin, WANG Bo, CHEN Haibing, YE Xuannan, SHI Tao 2024, 65 (8):  1991-1996.  DOI: 10.16178/j.issn.0528-9017.20230319 Abstract ( 78 )   HTML ( 6 )   PDF (7280KB) ( 202 )   Quzhou City is the birthplace of the “Agricultural Technology 110” agricultural service system. In the new era, especially under the wave of digital reform in Zhejiang Province, how to build a new era digital service system for agriculture, rural areas, and farmers to support the construction of an agricultural power has become a major issue that requires in-depth thinking and planning. This article analyzes the path exploration and achievements of Quzhou City in building a new era of digital “agriculture, rural areas, and farmers” service system, clarifies the current problems and challenges, and proposes the next direction of work. Figures and Tables | References | Related Articles | Metrics