QTL mapping and analysis of hypocotyl color in Brassica rapa ssp. chinensis

doi:10.16178/j.issn.0528-9017.20250358

Abstract

Abstract:

Hypocotyl color is of great significance for the improvement of traits in Brassica rapa ssp. chinensis. The aim of this study was to use bulky segregant analysis sequencing (BSA-seq) technology to preliminarily locate genes related to the regulation of hypocotyl color in Brassica rapa ssp. chinensis. The F₂ population was constructed for phenotype analysis using the green hypocotyl germplasm 99-70F (11X) as the female parent and the purple hypocotyl germplasm 99-70M (11Y) as the male parent of Brassica rapa ssp. chinensis. BSA-seq technology was used to preliminarily locate the target gene on chromosome A09, and competitive allele specific PCR (KASP) markers were developed within the quantitative trait locus (QTL) interval. The genotyping results showed that the loci A09:7 694 558 and A09:8 145 013 were highly correlated with the hypocotyl color of the F₂ population. This study lay a good foundation for the fine mapping of genes related to the regulation of hypocotyl color and molecular marker-assisted selection breeding of Brassica rapa ssp. chinensis.

Key words: Brassica rapa ssp. chinensis, hypocotyl, F₂ population, QTL mapping

CLC Number:

S634.3

HU Jinbin, HAN Nannan, CHEN Qin, WU Xinsheng. QTL mapping and analysis of hypocotyl color in Brassica rapa ssp. chinensis[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(11): 2617-2621.

Figures/Tables 6

References 14

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样品混池	净数据/ G	Q20含量/ %	Q30含量/ %	GC含量/ %	比对率/ %
11X	6.21	99.25	97.13	37.51	96.86
11Y	6.13	99.33	97.26	38.50	98.57
L11	10.62	99.41	97.49	38.81	95.81
Z11	12.31	99.40	97.52	38.96	95.33

样品混池	净数据/ G	Q20含量/ %	Q30含量/ %	GC含量/ %	比对率/ %
11X	6.21	99.25	97.13	37.51	96.86
11Y	6.13	99.33	97.26	38.50	98.57
L11	10.62	99.41	97.49	38.81	95.81
Z11	12.31	99.40	97.52	38.96	95.33

单株号	位点1	位点2	表型	单株号	位点1	位点2	表型
单株号	A09:7 694 558	A09:8 145 013	表型	单株号	A09:7 694 558	A09:8 145 013	表型
11X	Y:Y	X:X	绿色	11Y	X:X	Y:Y	紫色
11F₁	X:Y	X:Y	紫色	11F₂-21	X:Y	X:Y	紫色
11F₂-1	Y:Y	X:X	绿色	11F₂-22	X:X	Y:Y	紫色
11F₂-2	Y:Y	X:X	绿色	11F₂-23	X:Y	X:Y	紫色
11F₂-3	Y:Y	X:X	绿色	11F₂-24	X:Y	X:Y	紫色
11F₂-4	Y:Y	X:X	绿色	11F₂-25	X:X	Y:Y	紫色
11F₂-5	Y:Y	X:X	绿色	11F₂-26	X:Y	X:Y	紫色
11F₂-6	Y:Y	X:X	绿色	11F₂-27	X:X	Y:Y	紫色
11F₂-7	Y:Y	X:X	绿色	11F₂-28	X:Y	X:Y	紫色
11F₂-8	Y:Y	X:X	绿色	11F₂-29	X:Y	X:Y	紫色
11F₂-9	Y:Y	X:X	绿色	11F₂-30	X:Y	X:Y	紫色
11F₂-10	Y:Y	X:X	绿色	11F₂-31	X:X	X:Y	紫色
11F₂-11	X:Y	X:Y	绿色	11F₂-32	X:Y	X:Y	紫色
11F₂-12	Y:Y	X:X	绿色	11F₂-33	X:X	Y:Y	紫色
11F₂-13	X:Y	X:Y	绿色	11F₂-34	X:Y	X:Y	紫色
11F₂-14	Y:Y	X:X	绿色	11F₂-35	X:X	Y:Y	紫色
11F₂-15	Y:Y	X:X	绿色	11F₂-36	X:X	Y:Y	紫色
11F₂-16	X:Y	X:Y	绿色	11F₂-37	X:X	Y:Y	紫色
11F₂-17	Y:Y	X:X	绿色	11F₂-38	X:Y	X:Y	紫色
11F₂-18	Y:Y	X:X	绿色	11F₂-39	X:Y	X:Y	紫色
11F₂-19	Y:Y	X:X	绿色	11F₂-40	X:Y	X:Y	紫色
11F₂-20	Y:Y	X:X	绿色

单株号	位点1	位点2	表型	单株号	位点1	位点2	表型
单株号	A09:7 694 558	A09:8 145 013	表型	单株号	A09:7 694 558	A09:8 145 013	表型
11X	Y:Y	X:X	绿色	11Y	X:X	Y:Y	紫色
11F₁	X:Y	X:Y	紫色	11F₂-21	X:Y	X:Y	紫色
11F₂-1	Y:Y	X:X	绿色	11F₂-22	X:X	Y:Y	紫色
11F₂-2	Y:Y	X:X	绿色	11F₂-23	X:Y	X:Y	紫色
11F₂-3	Y:Y	X:X	绿色	11F₂-24	X:Y	X:Y	紫色
11F₂-4	Y:Y	X:X	绿色	11F₂-25	X:X	Y:Y	紫色
11F₂-5	Y:Y	X:X	绿色	11F₂-26	X:Y	X:Y	紫色
11F₂-6	Y:Y	X:X	绿色	11F₂-27	X:X	Y:Y	紫色
11F₂-7	Y:Y	X:X	绿色	11F₂-28	X:Y	X:Y	紫色
11F₂-8	Y:Y	X:X	绿色	11F₂-29	X:Y	X:Y	紫色
11F₂-9	Y:Y	X:X	绿色	11F₂-30	X:Y	X:Y	紫色
11F₂-10	Y:Y	X:X	绿色	11F₂-31	X:X	X:Y	紫色
11F₂-11	X:Y	X:Y	绿色	11F₂-32	X:Y	X:Y	紫色
11F₂-12	Y:Y	X:X	绿色	11F₂-33	X:X	Y:Y	紫色
11F₂-13	X:Y	X:Y	绿色	11F₂-34	X:Y	X:Y	紫色
11F₂-14	Y:Y	X:X	绿色	11F₂-35	X:X	Y:Y	紫色
11F₂-15	Y:Y	X:X	绿色	11F₂-36	X:X	Y:Y	紫色
11F₂-16	X:Y	X:Y	绿色	11F₂-37	X:X	Y:Y	紫色
11F₂-17	Y:Y	X:X	绿色	11F₂-38	X:Y	X:Y	紫色
11F₂-18	Y:Y	X:X	绿色	11F₂-39	X:Y	X:Y	紫色
11F₂-19	Y:Y	X:X	绿色	11F₂-40	X:Y	X:Y	紫色
11F₂-20	Y:Y	X:X	绿色