Effect of high temperature on seedling growth of grafted watermelon

doi:10.16178/j.issn.0528-9017.20250472

Abstract

Abstract:

In order to investigate the effect of high temperature on seedling growth and development of grafted watermelon, this study used the watermelon variety Zhemi 8 as the scion and Yezhuang 1 as the stock to construct grafted seedlings. Self-rooted seedlings were used as the control, and their physiological changes were analyzed after 5 days of high temperature treatment at 42 ℃. The results showed that the thickness of hypocotyls in grafted seedlings was significantly (P<0.05) less inhibited by high temperature than in self-rooted seedlings. The decrease in SPAD value of true leaves of grafted seedlings was smaller than that of self-rooted seedlings under high temperature, and the MDA content of grafted seedlings decreased by 44.9% compared with self-rooted seedlings under high temperature. The POD activity of grafted seedlings increased by 25.0% under high temperature compared with normal temperature, and the CAT activity did not change significantly compared with normal temperature. However, the CAT activity of self-rooted seedlings increased by 38.2% under high temperature compared with normal temperature. The soluble sugar content of grafted seedlings was 32.4% higher than that of self-rooted seedlings under high temperature. The grafting reshapes the high-temperature stress response network through stable photosynthetic mechanisms, strengthened antioxidant enzyme defense, and optimized distribution of osmoregulatory substances. This study provides a theoretical basis for the breeding of heat-resistant stock and stress-resistant cultivation.

Key words: watermelon, grafting, high temperature, antioxidant enzyme activity, hypocotyl

CLC Number:

S651

XING Nailin, YAN Leiyan, HUANG Yunping, WANG Yuhong. Effect of high temperature on seedling growth of grafted watermelon[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2395-2399.

Figures/Tables 2

References 25

[1]	刘广, 羊杏平, 徐锦华, 等. 西瓜砧木育种研究进展[J]. 江苏农业科学, 2018, 46(23):23-26.
[2]	刘润秋, 张红梅, 徐敬华, 等. 砧木对嫁接西瓜生长及品质的影响[J]. 上海交通大学学报(农业科学版), 2003, 21(4):289-294.
[3]	任慧转, 周海姣, 尧甜, 等. 不同砧木对西瓜断根嫁接苗生长发育的影响[J]. 中国瓜菜, 2024, 37(7): 107-110, 143.
[4]	MUHAMMAD I, SHALMANI A, ALI M, et al. Mechanisms regulating the dynamics of photosynthesis under abiotic stresses[J]. Frontiers in Plant Science, 2021, 11: 615942.
[5]	WENG J Y, REHMAN A, LI P L, et al. Physiological and transcriptomic analysis reveals the responses and difference to high temperature and humidity stress in two melon genotypes[J]. International Journal of Molecular Sciences, 2022, 23(2): 734.
[6]	HAN Y Y, FAN S X, ZHANG Q, et al. Effect of heat stress on the MDA, proline and soluble sugar content in leaf lettuce seedlings[J]. Agricultural Sciences, 2013, 4(5): 112-115.
[7]	刘金祥, 谭杏婵, 刘家琼. 高温胁迫对香根草含水量、膜透性、脯氨酸和叶绿素含量的影响[J]. 草原与草坪, 2005, 25(6): 58-61.
[8]	靳路真, 王洋, 张伟, 等. 高温胁迫对不同耐性大豆品种生理生化的影响[J]. 大豆科学, 2019, 38(1): 63-71.
[9]	VIJAYAKUMAR A, BEENA R. Alterations in carbohydrate metabolism and modulation of thermo-tolerance in tomato under heat stress[J]. International Journal of Environment and Climate Change, 2023, 13(9): 2798-2818.
[10]	SUN J Q, QI L L, LI Y N, et al. PIF4-mediated activation of YUCCA8 expression integrates temperature into the auxin pathway in regulating Arabidopsis hypocotyl growth[J]. PLoS Genetics, 2012, 8(3): e1002594.
[11]	WANG Q, ZHU Z Q. Light signaling-mediated growth plasticity in Arabidopsis grown under high-temperature conditions[J]. Stress Biology, 2022, 2(1): 53.
[12]	郝婷, 朱月林, 丁小涛, 等. 根际高温胁迫对5种瓜类作物生长及叶片光合和叶绿素荧光参数的影响[J]. 植物资源与环境学报, 2014, 23(2): 65-73.
[13]	WEI H, ZHOU J, XU C, et al. Identification and characterization of an OSH1 thiol reductase from Populus trichocarpa[J]. Cells, 2020, 9(1): 76.
[14]	ZHOU Q, ZHAO F, ZHANG H H, et al. Responses of the growth, photosynthetic characteristics, endogenous hormones and antioxidant activity of Carpinus betulus L. seedlings to different light intensities[J]. Frontiers in Plant Science, 2022, 13: 1055984.
[15]	孟凡鲁. 甜瓜中间砧对嫁接西瓜生长和果实品质的影响[D]. 泰安: 山东农业大学, 2012.
[16]	白雪, 高芳, 杨炎昌, 等. 砧木嫁接对高温胁迫下黄瓜幼苗光合器官和叶绿体抗氧化系统的影响[C]// 中国园艺学会2017年论文摘要集. 昆明, 2017: 140.
[17]	王金玉. 砧木选择对嫁接西瓜生长及品质的影响[D]. 泰安: 山东农业大学, 2006.
[18]	ALLAKHVERDIEV S I, KRESLAVSKI V D, KLIMOV V V, et al. Heat stress: an overview of molecular responses in photosynthesis[J]. Photosynthesis Research, 2008, 98(1): 541-550.
[19]	YAN Y Y, DUAN F Y, LI X, et al. Photosynthetic capacity and assimilate transport of the lower canopy influence maize yield under high planting density[J]. Plant Physiology, 2024, 195(4): 2652-2667.
[20]	杨卫丽, 黄福灯, 曹珍珍, 等. 高温胁迫对水稻光合PSⅡ 系统伤害及其与叶绿体D1蛋白间关系[J]. 作物学报, 2013, 39(6): 1060-1068.
[21]	柯梅, 侯钰荣, 魏鹏, 等. 干旱胁迫下心叶驼绒藜生理响应特性[J]. 草业科学, 2023, 40(5): 1349-1357.
[22]	潘晓娇, 郑春芳, 刘伟成, 等. 钛铁试剂对低温胁迫及恢复后秋茄幼苗光合生理特性的影响[J]. 安徽农业科学, 2022, 50(12): 120-123, 140.
[23]	郁万文, 曹福亮. 高温胁迫下银杏叶片部分渗透调节物质的动态变化[J]. 福建林业科技, 2008, 35(2): 126-128, 141.
[24]	王国霞, 陈丽培, 寇刘秀, 等. 高温胁迫对25个油茶品种渗透调节物质的影响[J]. 河南农业科学, 2012, 41(4): 59-62.
[25]	DEVI P, LUKAS S, MILES C. Advances in watermelon grafting to increase efficiency and automation[J]. Horticulturae, 2020, 6(4): 88.

处理	接穗下胚轴长/cm	接穗下胚轴粗/mm	子叶SPAD值	真叶SPAD值	子叶颜色L^*值
YZ1/ZM8-T	3.10±0.06 d	2.62±0.04 ab	61.38±0.68 b	47.70±0.51 b	50.40±0.09 a
YZ1/ZM8-CK	3.30±0.03 c	2.69±0.06 a	63.24±0.69 a	48.64±0.69 b	46.98±0.57 b
ZM8-T	6.56±0.08 a	2.41±0.02 c	62.69±0.50 ab	48.06±0.15 b	50.20±0.85 a
ZM8-CK	6.24±0.11 b	2.53±0.03 b	58.07±0.46 c	51.93±0.10 a	47.59±0.59 b
处理	子叶颜色a^*值	子叶颜色b^*值	真叶颜色L^*值	真叶颜色a^*值	真叶颜色b^*值
YZ1/ZM8-T	-7.65±0.52 a	25.50±0.36 a	45.92±0.39 a	-6.10±0.36 a	17.38±0.57 a
YZ1/ZM8-CK	-8.57±0.44 b	20.36±0.07 c	45.96±0.44 a	-7.91±0.09 c	16.96±0.30 a
ZM8-T	-7.23±0.09 a	21.44±0.54 b	43.19±0.06 b	-5.74±0.12 a	14.53±0.45 b
ZM8-CK	-7.86±0.25 ab	19.96±0.04 c	43.14±0.27 b	-6.83±0.18 b	14.45±0.33 b

处理	接穗下胚轴长/cm	接穗下胚轴粗/mm	子叶SPAD值	真叶SPAD值	子叶颜色L^*值
YZ1/ZM8-T	3.10±0.06 d	2.62±0.04 ab	61.38±0.68 b	47.70±0.51 b	50.40±0.09 a
YZ1/ZM8-CK	3.30±0.03 c	2.69±0.06 a	63.24±0.69 a	48.64±0.69 b	46.98±0.57 b
ZM8-T	6.56±0.08 a	2.41±0.02 c	62.69±0.50 ab	48.06±0.15 b	50.20±0.85 a
ZM8-CK	6.24±0.11 b	2.53±0.03 b	58.07±0.46 c	51.93±0.10 a	47.59±0.59 b
处理	子叶颜色a^*值	子叶颜色b^*值	真叶颜色L^*值	真叶颜色a^*值	真叶颜色b^*值
YZ1/ZM8-T	-7.65±0.52 a	25.50±0.36 a	45.92±0.39 a	-6.10±0.36 a	17.38±0.57 a
YZ1/ZM8-CK	-8.57±0.44 b	20.36±0.07 c	45.96±0.44 a	-7.91±0.09 c	16.96±0.30 a
ZM8-T	-7.23±0.09 a	21.44±0.54 b	43.19±0.06 b	-5.74±0.12 a	14.53±0.45 b
ZM8-CK	-7.86±0.25 ab	19.96±0.04 c	43.14±0.27 b	-6.83±0.18 b	14.45±0.33 b

处理	MDA含量/ (nmol·g^-1)	可溶性糖含量/ (mg·g^-1)	POD活性/ (U·g^-1)	CAT活性/ (mmol·min^-1·g^-1)
YZ1/ZM8-T	29.31±0.62 b	5.27±0.23 a	569.79±30.84 a	10.07±0.13 a
YZ1/ZM8-CK	20.71±0.52 c	3.46±0.05 b	455.66±4.66 b	10.42±0.57 a
ZM8-T	53.15±0.89 a	3.98±0.04 b	398.40±12.36 c	11.40±0.27 a
ZM8-CK	26.21±1.07 bc	3.40±0.14 b	457.94±9.02 b	8.25±1.16 b

处理	MDA含量/ (nmol·g^-1)	可溶性糖含量/ (mg·g^-1)	POD活性/ (U·g^-1)	CAT活性/ (mmol·min^-1·g^-1)
YZ1/ZM8-T	29.31±0.62 b	5.27±0.23 a	569.79±30.84 a	10.07±0.13 a
YZ1/ZM8-CK	20.71±0.52 c	3.46±0.05 b	455.66±4.66 b	10.42±0.57 a
ZM8-T	53.15±0.89 a	3.98±0.04 b	398.40±12.36 c	11.40±0.27 a
ZM8-CK	26.21±1.07 bc	3.40±0.14 b	457.94±9.02 b	8.25±1.16 b