Abstract:

Zixia 65 is a new variety of Brassica oleracea L. var. botrytis Linnaeus with purple flower heads. The seed production yield and seed purity of a new variety directly affect its application and promotion in production. Therefore, studying the efficient seed production technology and genetic purity identification technology of Zixia 65 is of great significance for the demonstration and promotion of this variety. This study investigated the effects of the planting proportion of paternal lines and the number of branches left on the yield and related traits of Zixia 65 using Ogura male sterile lines. Competitive allele specific PCR (KASP) markers were developed based on a large amount of resequencing single nucleotide polymorphism (SNP) data, and a high-throughput, low-cost molecular marker purity rapid identification technology was developed. The results showed that when the planting proportion of paternal lines was 2∶3 and one main branch was left, the seed yield was the highest, equivalent to 23.3 kg per 667 m². When two main branches were left, there were more branches and pods per plant, but fewer seeds per pod. Three KASP markers S011, S012 and S026 were developed to accurately identify the genetic purity of the Zixia 65 hybrid.

Key words: Brassica oleracea L. var. botrytis Linnaeus, purple, Zixia 65, seed production, genetic purity