Journal of Zhejiang Agricultural Sciences ›› 2026, Vol. 67 ›› Issue (2): 346-355.DOI: 10.16178/j.issn.0528-9017.20240456

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Screening and comprehensive evaluation of salt tolerance in sweetpotato during seedling stage

ZHANG Liang1(), MA Wenqing2, ZHONG Ziyu1, AN Yanbo2, LU Guoquan2, CUI Peng2()   

  1. 1.Jinhua Academy of Agricultural Sciences,Jinhua 321000,Zhejiang
    2.College of Advanced Agricultural Sciences,Zhejiang A&F University,Hangzhou 311300,Zhejiang
  • Received:2024-06-03 Online:2026-02-28 Published:2026-03-07

Abstract:

In order to screen out differently salt-tolerant sweetpotato materials,15 sweetpotato varieties (lines) were selected as experimental materials. The effects of salt stress on the physiology and biochemistry of sweetpotato were analyzed by determining the activity of antioxidant enzymes,photosynthetic parameters and expression of related salt stress response genes. The results showed that under 200 mmol·L-1 NaCl treatment,superoxide dismutase (SOD) activities,malondialdehyde (MDA) content and the Na+/K+ of different sweetpotato varieties increased significantly compared with the control,yet net photosynthetic rate,transpiration rate and stomatal conductance were significantly decreased. The expression levels of IbSOS1IbNHX1 and IbAKT1 genes in 15 varieties (lines) were significantly higher than those in control,while IbHKT2;1 in those showed the opposite trend. Meanwhile,the ultrastructure demonstrated that the cell wall was thin and missed,the mitochondrial structure was seriously damaged,the chloroplast structure was deformed,the grana lamella was reduced,and the internal starch granules were enlarged. Based on the above results,the salt tolerance of the 15 varieties (lines) could be divided into three categories. Xushu 18,ZNLSP2017-1-9 and ZNLSP2017-5-20 were identified salt-tolerant. The second category included ZNLSP2017-1-8,ZNLSP2017-1-12,ZNLSP2017-1-16,ZNLSP2017-1-39,ZNLSP2017-2-10,ZNLSP2017-5-11,Xinxiang,Zheshu 132 and Zheshu 75,which were identified moderately salt-sensitive. ZNLSP2017-2-12,Ningzishu 8 and Zhezishu 3 were identified salt-sensitive. This study can provide germplasm resources for the salt-tolerant breeding and theoretical basis for the evaluation of salt-tolerant sweetpotato during seedling stage.

Key words: sweetpotato, salt tolerance screening, photosynthetic parameters, gene expression, ultrastructure

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