Journal of Zhejiang Agricultural Sciences ›› 2022, Vol. 63 ›› Issue (6): 1345-1350.DOI: 10.16178/j.issn.0528-9017.20212886
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Received:2021-08-19
Online:2022-06-11
Published:2022-06-14
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URL: http://www.zjnykx.cn/EN/10.16178/j.issn.0528-9017.20212886
| 农药 | 最适pH | 最适抗体浓度/ (μg·mL-1) | 实际抗体 用量/μg | ||
|---|---|---|---|---|---|
| 目测法 | 分光光度法 | 目测法 | 分光光度法 | ||
| 嘧菌酯 | 7.5 | 8.5 | 50.0 | 50.0 | 6.0 |
| 啶虫脒 | 7.5 | 7.5 | 31.2 | 31.2 | 3.8 |
| 吡虫啉 | 7.5 | 7.5 | 62.5 | 48.8 | 5.8 |
| 丙溴磷 | 7.0 | 8.5 | 50.0 | 50.0 | 6.0 |
| 多菌灵 | 8.5 | 8.5 | 50.0 | 100.0 | 12.0 |
| 农药 | 最适pH | 最适抗体浓度/ (μg·mL-1) | 实际抗体 用量/μg | ||
|---|---|---|---|---|---|
| 目测法 | 分光光度法 | 目测法 | 分光光度法 | ||
| 嘧菌酯 | 7.5 | 8.5 | 50.0 | 50.0 | 6.0 |
| 啶虫脒 | 7.5 | 7.5 | 31.2 | 31.2 | 3.8 |
| 吡虫啉 | 7.5 | 7.5 | 62.5 | 48.8 | 5.8 |
| 丙溴磷 | 7.0 | 8.5 | 50.0 | 50.0 | 6.0 |
| 多菌灵 | 8.5 | 8.5 | 50.0 | 100.0 | 12.0 |
| 农药 名称 | 抗原浓度/ (mg·mL-1) | 二抗浓度/ (mg·mL-1) | 金标抗体 稀释比例 | 标样浓度/ (μg·mL-1) | 抗原浓度/ (mg·mL-1) | 二抗浓度/ (mg·mL-1) | 金标抗体 稀释比例 | 标样浓度/ (μg·mL-1) | 抗原浓度/ (mg·mL-1) | 二抗浓度/ (mg·mL-1) | 金标抗体 稀释比例 | 标样浓度/ (μg·mL-1) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 0.50 | 0 | 0.50 | 0 | 0.50 | |||||||||||
| 嘧菌酯 | 0.60 | 0.50 | 1∶5 | - | + | 0.30 | 1.00 | 1∶5 | - | ± | 0.30 | 0.50 | 1∶2 | - | ± | |
| 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶4 | - | + | ||
| 0.15 | 0.50 | 1∶5 | ± | + | 0.30 | 0.25 | 1∶5 | - | + | 0.30 | 0.50 | 1∶8 | - | + | ||
| 啶虫脒 | 0.40 | 0.50 | 1∶5 | - | + | 0.20 | 0.80 | 1∶5 | - | ± | 0.20 | 0.40 | 1∶2 | - | ± | |
| 0.20 | 0.50 | 1∶5 | - | + | 0.20 | 0.40 | 1∶5 | - | + | 0.20 | 0.40 | 1∶5 | - | + | ||
| 0.10 | 0.50 | 1∶5 | ± | + | 0.20 | 0.20 | 1∶5 | - | + | 0.20 | 0.40 | 1∶10 | - | + | ||
| 吡虫啉 | 0.60 | 1.00 | 1∶2 | - | + | 0.60 | 2.00 | 1∶2 | - | ± | 0.60 | 1.00 | 1∶1 | - | ± | |
| 0.30 | 1.00 | 1∶2 | ± | + | 0.60 | 1.00 | 1∶2 | - | + | 0.60 | 1.00 | 1∶2 | - | + | ||
| 0.15 | 1.00 | 1∶2 | ± | + | 0.60 | 0.50 | 1∶2 | - | + | 0.60 | 1.00 | 1∶5 | - | + | ||
| 丙溴磷 | 1.00 | 0.50 | 1∶5 | - | + | 0.50 | 1.00 | 1∶5 | - | ± | 0.50 | 0.50 | 1∶2 | - | ± | |
| 0.50 | 0.50 | 1∶5 | - | + | 0.50 | 0.50 | 1∶5 | - | + | 0.50 | 0.50 | 1∶4 | - | + | ||
| 0.25 | 0.50 | 1∶5 | ± | + | 0.50 | 0.25 | 1∶5 | - | + | 0.50 | 0.50 | 1∶8 | - | + | ||
| 多菌灵 | 0.60 | 0.50 | 1∶5 | - | + | 0.30 | 1.00 | 1∶5 | - | ± | 0.30 | 0.50 | 1∶2 | - | ± | |
| 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶4 | - | + | ||
| 0.15 | 0.50 | 1∶5 | ± | + | 0.30 | 0.25 | 1∶5 | - | + | 0.30 | 0.50 | 1∶8 | - | + | ||
| 农药 名称 | 抗原浓度/ (mg·mL-1) | 二抗浓度/ (mg·mL-1) | 金标抗体 稀释比例 | 标样浓度/ (μg·mL-1) | 抗原浓度/ (mg·mL-1) | 二抗浓度/ (mg·mL-1) | 金标抗体 稀释比例 | 标样浓度/ (μg·mL-1) | 抗原浓度/ (mg·mL-1) | 二抗浓度/ (mg·mL-1) | 金标抗体 稀释比例 | 标样浓度/ (μg·mL-1) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 0.50 | 0 | 0.50 | 0 | 0.50 | |||||||||||
| 嘧菌酯 | 0.60 | 0.50 | 1∶5 | - | + | 0.30 | 1.00 | 1∶5 | - | ± | 0.30 | 0.50 | 1∶2 | - | ± | |
| 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶4 | - | + | ||
| 0.15 | 0.50 | 1∶5 | ± | + | 0.30 | 0.25 | 1∶5 | - | + | 0.30 | 0.50 | 1∶8 | - | + | ||
| 啶虫脒 | 0.40 | 0.50 | 1∶5 | - | + | 0.20 | 0.80 | 1∶5 | - | ± | 0.20 | 0.40 | 1∶2 | - | ± | |
| 0.20 | 0.50 | 1∶5 | - | + | 0.20 | 0.40 | 1∶5 | - | + | 0.20 | 0.40 | 1∶5 | - | + | ||
| 0.10 | 0.50 | 1∶5 | ± | + | 0.20 | 0.20 | 1∶5 | - | + | 0.20 | 0.40 | 1∶10 | - | + | ||
| 吡虫啉 | 0.60 | 1.00 | 1∶2 | - | + | 0.60 | 2.00 | 1∶2 | - | ± | 0.60 | 1.00 | 1∶1 | - | ± | |
| 0.30 | 1.00 | 1∶2 | ± | + | 0.60 | 1.00 | 1∶2 | - | + | 0.60 | 1.00 | 1∶2 | - | + | ||
| 0.15 | 1.00 | 1∶2 | ± | + | 0.60 | 0.50 | 1∶2 | - | + | 0.60 | 1.00 | 1∶5 | - | + | ||
| 丙溴磷 | 1.00 | 0.50 | 1∶5 | - | + | 0.50 | 1.00 | 1∶5 | - | ± | 0.50 | 0.50 | 1∶2 | - | ± | |
| 0.50 | 0.50 | 1∶5 | - | + | 0.50 | 0.50 | 1∶5 | - | + | 0.50 | 0.50 | 1∶4 | - | + | ||
| 0.25 | 0.50 | 1∶5 | ± | + | 0.50 | 0.25 | 1∶5 | - | + | 0.50 | 0.50 | 1∶8 | - | + | ||
| 多菌灵 | 0.60 | 0.50 | 1∶5 | - | + | 0.30 | 1.00 | 1∶5 | - | ± | 0.30 | 0.50 | 1∶2 | - | ± | |
| 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶5 | - | + | 0.30 | 0.50 | 1∶4 | - | + | ||
| 0.15 | 0.50 | 1∶5 | ± | + | 0.30 | 0.25 | 1∶5 | - | + | 0.30 | 0.50 | 1∶8 | - | + | ||
| 农药 | 线性范围/ (mg·L-1) | 线性方程 | 相关系数 |
|---|---|---|---|
| 嘧菌酯 | 0.000 5~0.050 | y=7 250 168 x+11 127 | 0.998 6 |
| 啶虫脒 | y=16 772 539 x-4 818 | 0.999 9 | |
| 吡虫啉 | y=163 327 496 x-29 066 | 0.999 9 | |
| 丙溴磷 | y=47 263 162 x-31 346 | 0.999 4 | |
| 多菌灵 | 0.001~0.50 | y=344 394 x+1 567 | 0.999 7 |
| 农药 | 线性范围/ (mg·L-1) | 线性方程 | 相关系数 |
|---|---|---|---|
| 嘧菌酯 | 0.000 5~0.050 | y=7 250 168 x+11 127 | 0.998 6 |
| 啶虫脒 | y=16 772 539 x-4 818 | 0.999 9 | |
| 吡虫啉 | y=163 327 496 x-29 066 | 0.999 9 | |
| 丙溴磷 | y=47 263 162 x-31 346 | 0.999 4 | |
| 多菌灵 | 0.001~0.50 | y=344 394 x+1 567 | 0.999 7 |
| 农药 | 柑橘中农药浓度/(mg·L-1) | |||||
|---|---|---|---|---|---|---|
| 嘧菌酯 | 0.02 | 0.1 | 0.2 | 1.0 | 5.0 | 10.0 |
| - | - | + | + | + | + | |
| 啶虫脒 | 0.1 | 0.5 | 2.0 | 10.0 | 20.0 | 50.0 |
| - | + | + | + | + | + | |
| 吡虫啉 | 0.015 | 0.06 | 0.3 | 1.5 | 7.5 | 30.0 |
| - | - | + | + | + | + | |
| 丙溴磷 | 0.01 | 0.04 | 0.2 | 1.0 | 5.0 | 25.0 |
| - | - | + | + | + | + | |
| 多菌灵 | 0.01 | 0.06 | 0.3 | 1.5 | 7.5 | 30.0 |
| - | - | + | + | + | + | |
| 农药 | 柑橘中农药浓度/(mg·L-1) | |||||
|---|---|---|---|---|---|---|
| 嘧菌酯 | 0.02 | 0.1 | 0.2 | 1.0 | 5.0 | 10.0 |
| - | - | + | + | + | + | |
| 啶虫脒 | 0.1 | 0.5 | 2.0 | 10.0 | 20.0 | 50.0 |
| - | + | + | + | + | + | |
| 吡虫啉 | 0.015 | 0.06 | 0.3 | 1.5 | 7.5 | 30.0 |
| - | - | + | + | + | + | |
| 丙溴磷 | 0.01 | 0.04 | 0.2 | 1.0 | 5.0 | 25.0 |
| - | - | + | + | + | + | |
| 多菌灵 | 0.01 | 0.06 | 0.3 | 1.5 | 7.5 | 30.0 |
| - | - | + | + | + | + | |
| 农药 | 添加水平/(mg·kg-1) | 回收率/% | 相对标准差/% |
|---|---|---|---|
| 嘧菌酯 | 0.010 | 81 | 7.0 |
| 0.10 | 91 | 6.1 | |
| 1.00 | 78 | 5.2 | |
| 啶虫脒 | 0.010 | 96 | 1.2 |
| 0.10 | 103 | 0.7 | |
| 1.00 | 79 | 0.7 | |
| 吡虫啉 | 0.010 | 95 | 1.1 |
| 0.10 | 93 | 1.2 | |
| 1.00 | 88 | 1.3 | |
| 丙溴磷 | 0.010 | 78 | 2.8 |
| 0.10 | 77 | 3.1 | |
| 1.00 | 73 | 2.2 | |
| 多菌灵 | 0.10 | 89 | 3.3 |
| 0.50 | 87 | 5.0 | |
| 1.00 | 95 | 3.1 |
| 农药 | 添加水平/(mg·kg-1) | 回收率/% | 相对标准差/% |
|---|---|---|---|
| 嘧菌酯 | 0.010 | 81 | 7.0 |
| 0.10 | 91 | 6.1 | |
| 1.00 | 78 | 5.2 | |
| 啶虫脒 | 0.010 | 96 | 1.2 |
| 0.10 | 103 | 0.7 | |
| 1.00 | 79 | 0.7 | |
| 吡虫啉 | 0.010 | 95 | 1.1 |
| 0.10 | 93 | 1.2 | |
| 1.00 | 88 | 1.3 | |
| 丙溴磷 | 0.010 | 78 | 2.8 |
| 0.10 | 77 | 3.1 | |
| 1.00 | 73 | 2.2 | |
| 多菌灵 | 0.10 | 89 | 3.3 |
| 0.50 | 87 | 5.0 | |
| 1.00 | 95 | 3.1 |
| 农药 | 批次 | 空白基质组 | 农药加标组 | ||
|---|---|---|---|---|---|
| 重复1 | 重复2 | 重复3 | |||
| 嘧菌酯 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 啶虫脒 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 吡虫啉 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 丙溴磷 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 多菌灵 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 农药 | 批次 | 空白基质组 | 农药加标组 | ||
|---|---|---|---|---|---|
| 重复1 | 重复2 | 重复3 | |||
| 嘧菌酯 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 啶虫脒 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 吡虫啉 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 丙溴磷 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 多菌灵 | 1 | - | + | + | + |
| 2 | - | + | + | + | |
| 3 | - | + | + | + | |
| 农药 | 阴性样本 | 阳性样本 | 抽检样本 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 样品数 | 假阳性 个数 | 假阳性率/ % | 准确率/ % | 样品数 | 假阴性 个数 | 假阴性率/ % | 准确率/ % | 样品数 | 阳性数/ 假阴性数 | 阴性数/ 假阳性数 | 准确率/ % | ||
| 多菌灵 | 20 | 0 | 0 | 100 | 20 | 0 | 0 | 100 | 20 | 0/0 | 20/0 | 100 | |
| 啶虫脒 | 20 | 0 | 0 | 100 | 20 | 1 | 5 | 95 | 20 | 0/0 | 20/0 | 100 | |
| 吡虫啉 | 20 | 0 | 0 | 100 | 20 | 0 | 0 | 100 | 20 | 0/0 | 20/0 | 100 | |
| 丙溴磷 | 20 | 0 | 0 | 100 | 20 | 1 | 5 | 95 | 20 | 0/0 | 20/0 | 100 | |
| 嘧菌酯 | 20 | 0 | 0 | 100 | 20 | 2 | 10 | 90 | 20 | 0/0 | 20/0 | 100 | |
| 农药 | 阴性样本 | 阳性样本 | 抽检样本 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 样品数 | 假阳性 个数 | 假阳性率/ % | 准确率/ % | 样品数 | 假阴性 个数 | 假阴性率/ % | 准确率/ % | 样品数 | 阳性数/ 假阴性数 | 阴性数/ 假阳性数 | 准确率/ % | ||
| 多菌灵 | 20 | 0 | 0 | 100 | 20 | 0 | 0 | 100 | 20 | 0/0 | 20/0 | 100 | |
| 啶虫脒 | 20 | 0 | 0 | 100 | 20 | 1 | 5 | 95 | 20 | 0/0 | 20/0 | 100 | |
| 吡虫啉 | 20 | 0 | 0 | 100 | 20 | 0 | 0 | 100 | 20 | 0/0 | 20/0 | 100 | |
| 丙溴磷 | 20 | 0 | 0 | 100 | 20 | 1 | 5 | 95 | 20 | 0/0 | 20/0 | 100 | |
| 嘧菌酯 | 20 | 0 | 0 | 100 | 20 | 2 | 10 | 90 | 20 | 0/0 | 20/0 | 100 | |
| [1] | 熊亚波, 闫晓俊, 颜静, 等. 新型柑橘贮藏保鲜剂的研究进展[J]. 食品科学, 2015, 36(9):284-288. |
| [2] | CHHIKARA N, KOUR R, JAGLAN S, et al. Citrus medica:nutritional,phytochemical composition and health benefits-a review[J]. Food & Function, 2018, 9(4):1978-1992. |
| [3] |
ZHA J, WU X, GONG G L, et al. Pathway enzyme engineering for flavonoid production in recombinant microbes[J]. Metabolic Engineering Communications, 2019, 9:e00104.
DOI URL |
| [4] | 国家统计局农村社会经济调查司. 中国农村统计年鉴[M]. 北京: 中国统计出版社, 2020. |
| [5] |
DONG M F, WEN G Y, TANG H X, et al. Dissipation and safety evaluation of novaluron,pyriproxyfen,thiacloprid and tolfenpyrad residues in the Citrus-field ecosystem[J]. Food Chemistry, 2018, 269:136-141.
DOI URL |
| [6] |
BESIL N, PÉREZ-PARADA A, BOLOGNA F, et al. Dissipation of selected insecticides and fungicides applied during pre-harvest on mandarin and orange trees in Uruguay[J]. Scientia Horticulturae, 2019, 248:34-40.
DOI URL |
| [7] | ZHAO J L, TAN Z C, WEN Y, et al. Dissipation of fluazinam in Citrus groves and a risk assessment for its dietary intake[J]. Journal of the Science of Food and Agriculture, 2020, 100(5):2052-2056. |
| [8] |
LI Z X, ZHANG Y H, ZHAO Q Y, et al. Occurrence,temporal variation,quality and safety assessment of pesticide residues on Citrus fruits in China[J]. Chemosphere, 2020, 258:127381.
DOI URL |
| [9] | 林涛, 陈兴连, 刘兴勇, 等. QuEChERS-三重四极杆串联质谱测定柑橘中农药基质效应的方法研究[J]. 果树学报, 2021, 38(4):613-622. |
| [10] | 史晓亚, 高丽霞, 黄登宇. 快速检测技术在果蔬安全控制中的研究进展[J]. 食品安全质量检测学报, 2017, 8(3):882-889. |
| [11] | 张圆圆, 刘磊, 李娜, 等. 农药残留检测中不同蔬菜的基质效应[J]. 农药学学报, 2019, 21(3):327-337. |
| [12] | 蒲继锋, 潘虎, 代艳娜, 等. 气相色谱法测定茄果类蔬菜中12种有机磷类农药残留[J]. 食品安全质量检测学报, 2020, 11(4):1192-1196. |
| [13] |
NO H Y, KIM Y A, LEE Y T, et al. Cholinesterase-based dipstick assay for the detection of organophosphate and carbamate pesticides[J]. Analytica Chimica Acta, 2007, 594(1):37-43.
DOI URL |
| [14] |
SALAM F, PUAT N H A, RAHMAN G A, et al. Comparative study of pesticides analysis using enzyme inhibition sensor and gas chromatography methods[J]. Procedia Chemistry, 2016, 20:33-39.
DOI URL |
| [15] | 于基成, 边辞, 赵娜, 等. 酶抑制法快速检测蔬菜中有机磷农药残留[J]. 江苏农业科学, 2006(5):170-172. |
| [16] | 中华人民共和国国家卫生健康委员会, 中华人民共和国农业农村部, 国家市场监督管理总局. 食品安全国家标准食品中农药最大残留限量:GB 2763—2019[S], 北京: 中国农业出版社, 2019. |
| [17] | 王林, 王晶, 张莹, 等. 蔬菜中有机磷和氨基甲酸醋类农药残留量的快速检测方法研究[J]. 中国食品卫生杂志, 2003, 15(1):39-41. |
| [18] | 刘雅宁, 袁宝凤. 农药残留胶体金检测方法在蔬菜生产中的应用研究[J]. 陕西农业科学, 2014, 60(3):67-68. |
| [19] |
FRENS G. Preparation gold dispersions of varying particle size:controlled nucleation for the regulation of the particle size inmonodisperse gold solutions[J]. Nature Physical Science, 1973, 241(105):20-22.
DOI URL |
| [20] |
GEOGHEGAN W D. The effect of three variables on adsorption of rabbit IgG to colloidal gold[J]. The Journal of Histochemistry and Cytochemistry, 1988, 36(4):401-407.
DOI URL |
| [21] |
FOUBERT A, BELOGLAZOVA N V, DE SAEGER S. Comparative study of colloidal gold and quantum dots as labels for multiplex screening tests for multi-mycotoxin detection[J]. Analytica Chimica Acta, 2017, 955:48-57.
DOI URL |
| [22] |
ZHANG Y, PENG J, GUO P, et al. Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay[J]. Food and Agricultural Immunology, 2018, 29(1):711-721.
DOI URL |
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