Effects of exogenous GA3 and ABA on endogenous hormone regulation and conventional chemical composition of flue-cured tobacco

doi:10.16178/j.issn.0528-9017.20221178

Abstract

Abstract:

In this paper, the mechanism of exogenous hormones gibberellin (GA₃) and abscisic acid (ABA) was investigated from the hormone and gene levels, and the effects of exogenous hormones on the growth and development, yield and quality of flue-cured tobacco were studied, providing theoretical basis for field application of exogenous hormones. Field experiments were conducted with Zhongyan100 and NC71, and different concentrations of GA₃ and ABA were sprayed to determine the agronomic traits, gene expression, physiological characteristics, endogenous hormone content and chemical composition of tobacco leaves after roasting. The results showed that the height of ZY100 and NC71 plants increased by 20% and 31% respectively after GA₃ spraying compared with no GA₃ spraying. After spraying ABA, the height of ZY100 plant decreased by 17%, and the height of NC71 plant decreased by 10%. After spraying GA₃, the content of endogenous gibberellin in ZY100 and NC71 increased by 20% and 30%, respectively. After spraying exogenous GA₃, the gene expressions of CPS, GA20ox and PSY in ZY100 and NC71 tobacco plants were up-regulated, while the gene expressions of GA2ox were down-regulated. Spraying GA₃ increased the contents of chlorophyll and soluble protein in ZY100 and NC71, decreased the content of malondialdehyde, increased the total sugar content, potassium content, potassium chlorine ratio, decreased the nicotine content and chlorine content, and significantly improved the economic properties of flue-cured tobacco.

Key words: gibberellin, abscisic acid, flue-cured tobacco, gene expression, physiological characteristics, conventional chemical composition

CLC Number:

S572

LI Bo, CHEN Xiaoxiang, ZHANG Wenjun, ZHAI Zhengguang, LIU Huabing, JIANG Zhimin, XU Zhiqiang. Effects of exogenous GA₃ and ABA on endogenous hormone regulation and conventional chemical composition of flue-cured tobacco[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(1): 181-189.

Figures/Tables 9

References 22

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基因	引物序列(5'-3')
基因	上游引物	下游引物
NtL25	CCCCTCACCACAGAGTCTGC	AAGGGTGTTGTTGTCCTCAATCTT
PSY	CGATGATTTAGAAGTGAAGCCG	ACATACTTCGCCACAACG
CPS	AAGTGTGAATTAGGAATGTCGT	ATGCCACTTCAAATCCGATAG
GA20ox	GATACTCCGCTGAAGAAGACT	GATAAACATTCCCAAGATGGCT
GA2ox	AGCCATTAAATTCTTCTCCTCT	TACGACCAATCTGTTTATTGCC

基因	引物序列(5'-3')
基因	上游引物	下游引物
NtL25	CCCCTCACCACAGAGTCTGC	AAGGGTGTTGTTGTCCTCAATCTT
PSY	CGATGATTTAGAAGTGAAGCCG	ACATACTTCGCCACAACG
CPS	AAGTGTGAATTAGGAATGTCGT	ATGCCACTTCAAATCCGATAG
GA20ox	GATACTCCGCTGAAGAAGACT	GATAAACATTCCCAAGATGGCT
GA2ox	AGCCATTAAATTCTTCTCCTCT	TACGACCAATCTGTTTATTGCC

处理	总糖含量/%	烟碱含量/%	钾含量/%	氯含量/%	钾氯比
ZY100	22.85±0.81 b	2.64±0.31 ab	0.89±0.02 c	0.99±0.07 a	0.91±0.08 c
ZY100G	25.23±1.30 a	2.20±0.07 c	1.02±0.07 c	0.82±0.05 b	1.27±0.28 b
ZY100A	21.12±0.45 c	2.71±0.11 a	0.84±0.02 d	1.10±0.05 a	0.84±0.04 d
NC71	20.75±0.44 c	2.72±0.12 a	1.32±0.14 b	0.89±0.09 ab	1.48±0.18 b
NC71G	23.08±0.29 b	2.33±0.09 bc	1.56±0.03 a	0.62±0.16 c	2.64±0.65 a
NC71A	19.45±0.35 d	2.78±0.13 a	1.01±0.03 c	0.94±0.04 a	1.25±0.25 b

处理	总糖含量/%	烟碱含量/%	钾含量/%	氯含量/%	钾氯比
ZY100	22.85±0.81 b	2.64±0.31 ab	0.89±0.02 c	0.99±0.07 a	0.91±0.08 c
ZY100G	25.23±1.30 a	2.20±0.07 c	1.02±0.07 c	0.82±0.05 b	1.27±0.28 b
ZY100A	21.12±0.45 c	2.71±0.11 a	0.84±0.02 d	1.10±0.05 a	0.84±0.04 d
NC71	20.75±0.44 c	2.72±0.12 a	1.32±0.14 b	0.89±0.09 ab	1.48±0.18 b
NC71G	23.08±0.29 b	2.33±0.09 bc	1.56±0.03 a	0.62±0.16 c	2.64±0.65 a
NC71A	19.45±0.35 d	2.78±0.13 a	1.01±0.03 c	0.94±0.04 a	1.25±0.25 b

处理	每667 m²总产量/kg	每667 m²总产值/元	均价/(元·kg^-1)	中上等烟比例/%
ZY100	135.23±6.15 b	3 579.05±145.22 b	24.78±1.38 b	73.17±2.05 b
ZY100G	159.43±7.60 a	4 042.66±178.82 a	26.86±1.51 a	85.80±3.71 a
ZY100A	125.34±2.40 bc	2 945.41±124.22 c	22.41±0.88 c	68.24±1.98 c
NC71	127.57±3.40 bc	2 767.50±127.61 c	21.45±0.98 c	69.73±1.53 c
NC71G	144.93±10.82 ab	3 767.72±185.55 b	23.39±1.28 b	76.08±2.75 b
NC71A	114.56±2.1 c	2 415.21±125.61 d	19.47±0.79 d	63.15±1.24 d

Effects of exogenous GA₃ and ABA on endogenous hormone regulation and conventional chemical composition of flue-cured tobacco

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