Comparative analysis of chemical components and antioxidant capacity of Tetrastigma hemsleyanum based on different provenances:taking Fuyang District, Hangzhou City, Zhejiang Province for example

doi:10.16178/j.issn.0528-9017.20230956

Journal of Zhejiang Agricultural Sciences ›› 2024, Vol. 65 ›› Issue (2): 320-324.DOI: 10.16178/j.issn.0528-9017.20230956

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Comparative analysis of chemical components and antioxidant capacity of Tetrastigma hemsleyanum based on different provenances:taking Fuyang District, Hangzhou City, Zhejiang Province for example

Xigen CHEN¹(), Hanjing SUN², Lijun YANG³, Qiang MIAO², Luomei ZHU⁴, Xiaojun CAI²^,^*()

1. Fuchun Street Center for Regional Development and Management of Fuyang District, Hangzhou 311402, Zhejiang
2. Agriculture and Rural Bureau of Fuyang District, Hangzhou 311400, Zhejiang
3. Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang
4. Changkou Street Center for Regional Development and Management of Fuyang District,Hangzhou City, Hangzhou 311411, Zhejiang

Received:2023-09-23 Online:2024-02-11 Published:2024-02-28
Contact: Xiaojun CAI

Abstract

Abstract:

Based on the content of total flavonoids, total polyphenols and polysaccharides and the UPLC-Q-TOF-MS/MS component analysis, five provenances of Tetrastigma hemsleyanum found in Fuyang District, Hangzhou City, Zhejiang Province were detected with DPPH radical scavenging ability and ABTS radical scavenging ability as evaluation indexes. It was found that the total polysaccharides, flavonoids, and phenols content of Shizhu 1, 4, and 5 were significantly higher than those of Shizhu 2 and 3, But the free radical scavenging ability of DPPH and ABTS was better than that of Shizhu 4 and 5. The results could provide basic data support for the classification, preservation, identification and evaluation of Tetrastigma hemsleyanum germplasm resources in Fuyang District.

Key words: provenance, Tetrastigma hemsleyanum, chemical component, antioxidant capacity

CLC Number:

S567

Xigen CHEN, Hanjing SUN, Lijun YANG, Qiang MIAO, Luomei ZHU, Xiaojun CAI. Comparative analysis of chemical components and antioxidant capacity of Tetrastigma hemsleyanum based on different provenances:taking Fuyang District, Hangzhou City, Zhejiang Province for example[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(2): 320-324.

Figures/Tables 4

References 9

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样品	总多糖含量	总黄酮含量	总酚含量
石硃1号	145.830 128 2	92.827 586 2	119.513 274 3
石硃2号	117.689 102 6	55.586 206 9	66.563 421 8
石硃3号	106.631 410 3	69.954 023 0	90.752 212 4
石硃4号	130.125 000 0	108.344 827 6	130.722 713 9
石硃5号	176.182 692 3	114.781 609 2	118.480 826 0

样品	总多糖含量	总黄酮含量	总酚含量
石硃1号	145.830 128 2	92.827 586 2	119.513 274 3
石硃2号	117.689 102 6	55.586 206 9	66.563 421 8
石硃3号	106.631 410 3	69.954 023 0	90.752 212 4
石硃4号	130.125 000 0	108.344 827 6	130.722 713 9
石硃5号	176.182 692 3	114.781 609 2	118.480 826 0

保留时间/min	化合物	分子式	分子量	离子碎片	化合物类型
1.197	D-quinic acid	C₇H₁₂O₆	191.018 6	85.027 75、87.007 03	有机酸
3.402	Vanillic acid-1-O-apiofuranosyl glucose ester	C₁₉H₂₆O₁₃	461.130 2	108.022 2、123.045 7、152.011、167.034 3、461.131	黄酮类
6.420	Camelliaside β	C₃₂H₃₈O₁₉	725.192 9	227.034 09、255.029 34、284.032 29	黄酮类
6.417	Kaempferol-3-O-furanose-7-O rhamnosylglucoside	C₃₂H₃₈O₁₉	725.195 8	283.025 5、284.033 3、285.040 6、430.091 9、579.139 9、725.202	黄酮类
7.078	isoorientin-2″-O-rhamnoside	C₂₇H₃₀O₁₅	593.150 9	447.090 7、429.086 6	黄酮类
20.800	Lauryl hydrogen sulfate	C₁₂H₂₆O₄S	265.148 2	79.960 2、96.962 1、265.148 2	硫酸酯类

保留时间/min	化合物	分子式	分子量	离子碎片	化合物类型
1.197	D-quinic acid	C₇H₁₂O₆	191.018 6	85.027 75、87.007 03	有机酸
3.402	Vanillic acid-1-O-apiofuranosyl glucose ester	C₁₉H₂₆O₁₃	461.130 2	108.022 2、123.045 7、152.011、167.034 3、461.131	黄酮类
6.420	Camelliaside β	C₃₂H₃₈O₁₉	725.192 9	227.034 09、255.029 34、284.032 29	黄酮类
6.417	Kaempferol-3-O-furanose-7-O rhamnosylglucoside	C₃₂H₃₈O₁₉	725.195 8	283.025 5、284.033 3、285.040 6、430.091 9、579.139 9、725.202	黄酮类
7.078	isoorientin-2″-O-rhamnoside	C₂₇H₃₀O₁₅	593.150 9	447.090 7、429.086 6	黄酮类
20.800	Lauryl hydrogen sulfate	C₁₂H₂₆O₄S	265.148 2	79.960 2、96.962 1、265.148 2	硫酸酯类

样品	DPPH	ABTS
石硃1号	32.78	40.90
石硃2号	30.96	41.81
石硃3号	18.92	15.05
石硃4号	57.68	69.70
石硃5号	44.74	47.30

Comparative analysis of chemical components and antioxidant capacity of Tetrastigma hemsleyanum based on different provenances:taking Fuyang District, Hangzhou City, Zhejiang Province for example

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