Abstract:

In this study, the 90-day-old buds of Fritilaria unibracteata Hsiao et K.C.Hsia were used as experimental materials, and different concentrations of hormones and sugars were added to study their effects on the rooting of Fritilaria unibracteata Hsiao et K.C.Hsia in tissue culture, and the most suitable rooting medium for Fritilaria unibracteata Hsiao et K.C.Hsia was screened. The results showed that the rooting medium was MS+9.4 g·L^-1 car+0.5 mL·L^-1 NAA+0.5 mL·L^-1 IBA+30 g·L^-1 sucrose had the best rooting effect, with a rooting rate of 81.3%, a thick root system, and the largest number of effective roots, with an average of 3.4 roots per plant, and no pollution. This study provides a reference for the establishment of a fast propagation system for tissue culture and large-scale standardized production of excellent Fritilaria unibracteata Hsiao et K.C.Hsia seedlings in the future.

Key words: Fritilaria unibracteata Hsiao et K.C.Hsia, tissue culture, hormones, sugar, rooting