Identification of CHS gene in Chinese cabbage and transcriptional expression analysis under high nitrogen levels

doi:10.16178/j.issn.0528-9017.20230943

Abstract

Abstract:

In order to investigate the mechanism of exacerbation of Chinese cabbage petiole black spot disease caused by high nitrogen levels, the resistant and suscetible Chinese cabbage were treated with normal and high nitrogen levels, and sampled petioles were sampled at different time before and after treatment for transcriptome sequencing. The Chinese cabbage chalcone synthase (CHS) gene was identified and analyzed for different Chinese cabbage CHS genes at normal and high nitrogen levels. The differential expression between resistant and susceptible Chinese cabbage showed that a total of 7 CHS genes were identified, of which 3 genes (BrCHS1, BrCHS3, and BrCHS4) had higher expression levels under high nitrogen levels than the normal nitrogen levels, and the expression level of susceptible Chinese cabbage was higher than that of resistant Chinese cabbage under high nitrogen levels. Therefore, it is speculated that these three Chinese cabbage CHS genes may be related to the formation of Chinese cabbage petiole black spot disease. This study lays the foundation for revealing the mechanism of black spot disease on the petiole of Chinese cabbage.

Key words: Chinese cabbage, petiole black spot disease, chalcone synthetase, high nitrogen level, expression analysis

CLC Number:

S634.1

LEI Juanli, ZHAO Yanting, YUE Zhichen, TAO Peng, HU Qizan, LI Biyuan. Identification of CHS gene in Chinese cabbage and transcriptional expression analysis under high nitrogen levels[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(5): 1102-1107.

Figures/Tables 7

Table 1 Comparison of chalcone synthase genes in Chinese cabbage and Arabidopsis

序号	大白菜基因名称	基因号	碱基数	氨基酸数	同源性/%	e值
0	AtCHS	AT5G13930	1 577	395	—	—
1	BrCHS1	BraA02g005190.3C	1 452	394	95.455	0
2	BrCHS2	BraA02g039760.3C	1 269	394	88.131	0
3	BrCHS3	BraA03g005990.3C	1 458	396	95.214	0
4	BrCHS4	BraA06g019160.3C	1 698	350	72.500	6.12×10^-89
5	BrCHS5	BraA09g002250.3C	966	321	89.752	0
6	BrCHS6	BraA09g002260.3C	277	68	88.710	3.76×10^-35
7	BrCHS7	BraA10g024990.3C	1 263	395	95.707	0

Fig.1 Phylogenetic tree of CHS genes in Chinese cabbage and Arabidopsis

Fig.2 CHS gene structure in Chinese cabbage

Fig.3 Chromosomal localization of CHS gene in Chinese cabbage

Fig.4 Relative expression levels of CHS genes in resistant line under high nitrogen levels

Fig.5 Relative expression levels of CHS genes in susceptible line under high nitrogen levels

Fig.6 Relative expression levels of CHS genes in resistant and susceptible strains at different nitrogen levels

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