Determination of fenpyrazone in maize by high performance liquid chromatography-tandem mass spectrometry

doi:10.16178/j.issn.0528-9017.20231164

Abstract

Abstract:

This study established a detection method for fenpyrazone in maize using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). QuEChERS method was optimized for effective extraction and purification of fenpyrazone in samples. The results showed that there was a good linear relationship in the concentration range of 1-100 μg·L^-1 for fenpyrazone with correlation coefficient greater than 0.99. The limit of quantitation (LOQ) was 6 μg·kg^-1. At three spiked concentration levels of 6, 20 and 100 μg·kg^-1 for fenpyrazone, the average recoveries were 89.0%-90.4%, with relative standard deviations (RSDs) of 0.8%-2.5% (n=6). The method is simple, efficient, sensitive, accurate and suitable for determination of fenpyrazone in maize.

Key words: high performance liquid chromatography-tandem mass spectrometry, fenpyrazone, maize

CLC Number:

S481

SU Chunmin, BIAN Haitao, YONG Yanhua, LI Peng, DONG Ruinan. Determination of fenpyrazone in maize by high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(2): 471-474.

Figures/Tables 5

References 18

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时间/min	流动相A/%	流动相B/%
0	95	5
0.5	95	5
3.0	5	95
5.0	5	95
5.1	95	5
7.0	95	5

时间/min	流动相A/%	流动相B/%
0	95	5
0.5	95	5
3.0	5	95
5.0	5	95
5.1	95	5
7.0	95	5

母离子(m/z)	子离子(m/z)	去簇电压/V	碰撞能量/eV
563.2	123.1^*	70	23
	463.2	70	27

母离子(m/z)	子离子(m/z)	去簇电压/V	碰撞能量/eV
563.2	123.1^*	70	23
	463.2	70	27

物质	线性回归方程	相关系数r	基质效应
乙腈	y=1.110 94e5 x+1 096.336 88	0.999 98
基质	y=9.517 07e4 x-7 217.419 01	0.999 90	0.86