To determine the optimal harvesting periods for different citrus varieties in the hot and humid regions of Yunnan, this study focused on the main cultivars in Dehong area. Analysis of changes in soluble solids content, titratable acid content, single fruit weight, and edible rate began at the fruit color-turning stage. The results indicated that the Jinqiu sugar citrus matured and was ready for harvest by August 20th, with stable single fruit weight, longitudinal diameter, transverse diameter, edible rate, and titratable acid content, while soluble solids content increased slowly. Jinqiu sugar citrus had the earliest maturity period and maintained high quality for an extended time. The Bingtang orange matured in late November, with stable single fruit weight, longitudinal diameter, and transverse diameter, thin peel, high edible rate, and a high and stable solid-to-acid ratio. After January 20th, the edible rate and solid-to-acid ratio of Bingtang orange significantly decreased, making the optimal harvesting period from late November to late January. Sugar citrus, Hongjiang orange, and W. Murcott began to mature in early December, with stable single fruit weight, longitudinal diameter, and transverse diameter, high edible rate, and large solid-to-acid ratio. The edible rate of Sugar citrus and W. Murcott decreased significantly after January 20th, harvesting was needed to complete as soon as possible. The peel thickness of Hongjiang orange increased after March 5th, making it unsuitable for further harvesting. Tango and Honeymurcott began to mature in late December, with stable single fruit weight, longitudinal diameter, and transverse diameter, high edible rate, and large solid-to-acid ratio. The solid-to-acid ratio of Tango decreased after February 5th, requiring completion of harvesting by that date. The edible rate of Honeymurcott significantly decreased after March 5th, making it unsuitable for further harvesting. The soluble solids and solid-to-acid ratio of Orah were high by February 5th, indicating the start of the harvesting period, which could be concluded by April 5th when they reached their maximum. Seedless Orah could be harvested in late February when the edible rate was high, and soluble solids and solid-to-acid ratio were large. After March 5th, the peel thickness of Seedless Orah increased, making it unsuitable for further harvesting. This study provides a scientific basis for determining the fruit characteristics and harvesting periods of citrus in Dehong area.