Study on the effect of electrolytic oxidation technology for treating seawater on the microalgae cultivation

doi:10.16178/j.issn.0528-9017.20231163

Abstract

Abstract:

To investigate the effect of electrolytic oxidation technology for treating seawater on the growth and reproduction of Isochrysis galbana 8701, Platymonas helgolandica var. tsingtaoensis and Chaetoceros muelleri, three experimental groups were set up, including electrolytic oxidation sand filtration seawater EOW₂₅₀ (effective chlorine concentration of 250 mg·L^-1), electrolytic oxidation sand filtration seawater EOW₁₀₀ (effective chlorine concentration of 100 mg·L^-1), and bleaching powder disinfection sand filtration seawater (effective chlorine concentration of 35 mg·L^-1), with boiled sand filtration seawater as the control group. Single species cultivation was carried out on Isochrysis galbana 8701, Platymonas helgolandica var. tsingtaoensis and Chaetoceros muelleri for 10 days. The results showed that electrolytic oxidation technology for treating seawater was suitable for the cultivation of three types of marine microalgae. The group treated with electrolytic oxidation seawater EOW₁₀₀(effective chlorine concentration of 100 mg·L^-1) for Isochrysis galbana 8701 achieved the highest cell density of 437.7×10⁴ cell ·mL^-1 on the 10th day of cultivation, and significantly higher than the control group (P<0.05).There was no significant difference in the final cell density of Platymonas helgolandica var. tsingtaoensis among the treatment groups (P>0.05). However, there was a significant difference (P<0.05) in the final bacterial number between the electrolytic oxidation seawater EOW₂₅₀(effective chlorine concentration of 250 mg·L^-1), electrolytic oxidation seawater EOW₁₀₀(effective chlorine concentration of 100 mg·L^-1) groups and the bleaching powder group. There was no significant difference in the the final bacterial number of Chaetoceros muelleri among the treatment groups (P>0.05).In conclusion, electrolytic oxidation seawater EOW₂₅₀ (effective chlorine concentration of 250 mg·L^-1) could be used for the culture of Platymonas helgolandica var. tsingtaoensis, while electrolytic oxidation seawater EOW₁₀₀(effective chlorine concentration of 100 mg·L^-1) was suitable for the cultivation of Isochrysis galbana 8701, Platymonas helgolandica var. tsingtaoensis and Chaetoceros muelleri. The aim of the study is to treat sand filtered seawater with electrolytic oxidation for the cultivation of microalgae and to provide an experimental basis for the application of microalgae in large-scale production.

Key words: electrolytic oxidation, microalgae, seawater treatment, growth condition

CLC Number:

S963

LI Tengteng, QIN Nuoyun, XU Jinqi, LI Pengquan, CHEN Chen, FANG Jun. Study on the effect of electrolytic oxidation technology for treating seawater on the microalgae cultivation[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(2): 487-494.

Figures/Tables 11

References 21

[1]	张国维, 李勤慎, 邵东宏, 等. 微藻在水产养殖中的研究应用进展[J]. 中国水产, 2020(2):72-74.
[2]	赵伟, 高保燕, 黄罗冬, 等. 微藻及其生物活性成分在水产养殖中的营养价值、生理功能和抗病活性[J]. 饲料工业, 2019, 40(8):9-16.
[3]	舒蕾. 微藻生物在水产饲料中的应用[J]. 养殖与饲料, 2022, 21(3):69-71.
[4]	李色东, 刘涛. 饵料微藻在南美白对虾育苗中的应用[J]. 海洋与渔业, 2014(11):62-63.
[5]	陈自强, 寿鹿, 廖一波, 等. 微藻饵料对双壳贝类幼体生长影响的研究进展[J]. 科技通报, 2013, 29(7):46-55,67.
[6]	张继红, 任丹丹, 姜玉声, 等. 微藻营养价值及其在水产生物营养强化中的应用[J]. 食品工业科技, 2016, 37(20):371-376.
[7]	佟会军, 常顺, 范龙霞. 漂白粉在渔业生产中的应用[J]. 黑龙江水产, 2006(4):15,18.
[8]	YAN P P, DALIRI E B M, OH D H. New clinical applications of electrolyzed water: a review[J]. Microorganisms, 2021, 9(1): 136.
[9]	裴洛伟. 基于微电解和紫外协同的海水循环水养殖系统水处理效果研究[D]. 杭州: 浙江大学, 2016.
[10]	沈晓盛, 刘长军, 蔡友琼, 等. 电解海水的抑菌活性及对食品加工表面材料的消毒效果[J]. 微生物学通报, 2008, 35(11): 1833-1839.
[11]	VIJAYARAGHAVAN K, AHMAD D, BIN FADZIN T S. In situ hypochlorous acid generation for the treatment of brackish shrimp aquaculture wastewater[J]. Aquaculture Research, 2008, 39(5): 449-456.
[12]	JORQUERA M A, VALENCIA G, EGUCHI M, et al. Disinfection of seawater for hatchery aquaculture systems using electrolytic water treatment[J]. Aquaculture, 2002, 207(3/4): 213-224.
[13]	吴永华, 韩柏平, 王劲. 电场杀菌的物理效应与化学效应分析[J]. 工业用水与废水, 2007, 38(2): 45-47.
[14]	ALFAFARA C G, KAWAMORI T, NOMURA N, et al. Electrolytic removal of ammonia from brine wastewater: scale-up, operation and pilot-scale evaluation[J]. Journal of Chemical Technology & Biotechnology, 2004, 79(3): 291-298.
[15]	刘斌杰, 袁晓丽, 毛森, 等. 余氯比色计法与DPD分光法测定水中余氯的比较研究[J]. 计量与测试技术, 2016, 43(8):41-42,44.
[16]	詹静婷, 黄永梅, 雷金丽, 等. 湛江等鞭金藻培养基和培养模式优化的研究[J]. 基因组学与应用生物学, 2019, 38(3):1248-1255.
[17]	KOUZUMA A, WATANABE K. Exploring the potential of algae/bacteria interactions[J]. Current Opinion in Biotechnology, 2015, 33: 125-129.
[18]	乔洪金, 刘相全, 马晶晶, 等. 5株海洋微藻伴生细菌的分离鉴定与功能分析[J]. 安徽农业科学, 2012, 40(29): 14421-14424.
[19]	孔周雁, 张羽帆, 凌婷, 等. 球等鞭金藻三级培养过程中细菌群落多样性分析[J]. 核农学报, 2020, 34(3):506-514.
[20]	KAZAMIA E, CZESNICK H, NGUYEN T T, et al. Mutualistic interactions between vitamin B₁₂-dependent algae and heterotrophic bacteria exhibit regulation[J]. Environmental Microbiology, 2012, 14(6): 1466-1476.
[21]	王兴林. 有效氯对虾池生物毒性及其衰减的研究[D]. 湛江: 广东海洋大学, 2015.

消毒方法	藻细胞终密度/(10⁴ cell·mL^-1)
消毒方法	球等鞭金藻8701	青岛大扁藻	牟氏角毛藻
电解氧化水EOW₂₅₀(有效氯浓度250 mg·L^-1)	345.3±4.2 c	83.7±3.8 a	192.3±32.8 b
电解氧化水EOW₁₀₀(有效氯浓度100 mg·L^-1)	437.7±35.2 a	81.0±4.0 a	321.3±4.9 a
漂白粉(有效氯浓度35 mg·L^-1)	411.7±10.7 ab	86.7±5.8 a	331.7±26.1 a
煮沸	382.7±13.4 bc	90.7±7.2 a	309.3±12.7 a

消毒方法	藻细胞终密度/(10⁴ cell·mL^-1)
消毒方法	球等鞭金藻8701	青岛大扁藻	牟氏角毛藻
电解氧化水EOW₂₅₀(有效氯浓度250 mg·L^-1)	345.3±4.2 c	83.7±3.8 a	192.3±32.8 b
电解氧化水EOW₁₀₀(有效氯浓度100 mg·L^-1)	437.7±35.2 a	81.0±4.0 a	321.3±4.9 a
漂白粉(有效氯浓度35 mg·L^-1)	411.7±10.7 ab	86.7±5.8 a	331.7±26.1 a
煮沸	382.7±13.4 bc	90.7±7.2 a	309.3±12.7 a

消毒方法	比生长速率
消毒方法	球等鞭金藻8701	青岛大扁藻	牟氏角毛藻
电解氧化水EOW₂₅₀(有效氯浓度250 mg·L^-1)	0.45±0.09 a	0.44±0.04 a	0.50±0.02 a
电解氧化水EOW₁₀₀(有效氯浓度100 mg·L^-1)	0.30±0.04 b	0.35±0.10 a	0.37±0.06 b
漂白粉(有效氯浓度35 mg·L^-1)	0.32±0.07 b	0.20±0.06 b	0.43±0.06 ab
煮沸	0.20±0.02 c	0.30±0.11 ab	0.44±0.05 ab

消毒方法	比生长速率
消毒方法	球等鞭金藻8701	青岛大扁藻	牟氏角毛藻
电解氧化水EOW₂₅₀(有效氯浓度250 mg·L^-1)	0.45±0.09 a	0.44±0.04 a	0.50±0.02 a
电解氧化水EOW₁₀₀(有效氯浓度100 mg·L^-1)	0.30±0.04 b	0.35±0.10 a	0.37±0.06 b
漂白粉(有效氯浓度35 mg·L^-1)	0.32±0.07 b	0.20±0.06 b	0.43±0.06 ab
煮沸	0.20±0.02 c	0.30±0.11 ab	0.44±0.05 ab

消毒方法	细菌数量/(10⁶ CFU·mL^-1)
消毒方法	初始	最终
电解氧化水EOW₂₅₀(有效氯浓度250 mg·L^-1)	5.17±1.65 a	0.57±0.13 a
电解氧化水EOW₁₀₀(有效氯浓度100 mg·L^-1)	4.67±1.11 a	0.87±0.35 a
漂白粉(有效氯浓度35 mg·L^-1)	3.60±0.36 a	0.37±0.15 b
煮沸	3.20±2.02 a	0.73±0.20 a