Study on low temperature storage technology of the natural enemy insect Aenasius bambawalei Hayat

doi:10.16178/j.issn.0528-9017.20240188

Abstract

Abstract:

Aenasius bambawalei Hayat is an obligate dominant parasitoid of the invasive pest Phenacoccus solenopsis Tinsley in China, playing a significant role in biological control. This study firstly treated the pupae of Aenasius bambawalei Hayat with different temperatures and photoperiods for varying durations to explore optimal low temperature acclimation conditions. Subsequently, the pupae were stored at 4 ℃ for 40, 80, 120, 160, 200, 240, 280, 300 days to determine the optimal low temperature storage duration. The results showed that when the pupae were acclimated at 14 ℃ under a light/dark photoperiod of 8 h/16 h for 6 days, followed by refrigeration at 4 ℃ for 80 days, and then reared under conditions of 26-28 ℃, 70%-80% relative humidity, and a photoperiod of 16 h/8 h, their performance was equivalent to the control group. This method provides technical support for enhancing the application potential of Aenasius bambawalei Hayat.

Key words: Aenasius bambawalei Hayat, low temperature storage, obligate parasitism, biological control

CLC Number:

S433.3

ZHANG Juan, LIU Xiaotong, WANG Ke, DONG Wanying, FU Kaiyun, YANG Dong, GAO Jiancheng, HUANG Jun. Study on low temperature storage technology of the natural enemy insect Aenasius bambawalei Hayat[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(7): 1711-1714.

Figures/Tables 3

References 12

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光周期 (光照/黑暗)	活蛹数
光周期 (光照/黑暗)	12 ℃	14 ℃	16 ℃	18 ℃	20 ℃
8 h/16 h	8.00±0.58 ABc	23.33±0.33 Cb	24.00±1.15 Db	23.00±0.57 Eb	27.00±1.15 Da
10 h/14 h	8.67±0.33 ABe	24.00±1.15 Cd	26.00±1.15 Ab	25.33±0.33 Cc	27.33±0.33 Ca
12 h/12 h	10.00±0.67 Ad	22.33±0.33 Cc	24.67±0.33 Cb	25.00±0.57 Db	27.67±0.67 Ba
14 h/10 h	8.67±0.67 ABd	26.67±0.67 Ab	25.33±0.33 Bc	27.67±0.67 Aa	26.67±0.67 Eb
16 h/8 h	7.33±0.33 Bd	25.33±0.33 Bc	26.00±1.15 Ab	26.00±1.15 Bb	28.33±0.33 Aa
光周期 (光照/黑暗)	羽化率/%
光周期 (光照/黑暗)	12 ℃	14 ℃	16 ℃	18 ℃	20 ℃
8 h/16 h	33.27±10.39 Dd	4.29±0.06 De	54.19±0.20 Dc	65.17±0.88 Eb	83.98±0.70 Ca
10 h/14 h	46.30±1.85 Cd	11.04±1.01 Ce	64.06±0.84 Cc	67.13±0.87 Db	86.60±1.16 Ca
12 h/12 h	53.37±1.71 Bd	28.33±1.05 Be	71.61±0.39 Bc	78.70±0.92 Bb	92.76±0.17 Ba
14 h/10 h	61.67±0.83 Ac	30.04±0.73 Bd	72.36±0.36 Bb	73.52±0.55 Cb	100.00±0 Aa
16 h/8 h	58.93±1.79 Bd	35.54±0.46 Ae	76.83±1.03 Ac	88.42±0.52 Ab	96.47±0.04 ABa

光周期 (光照/黑暗)	活蛹数
光周期 (光照/黑暗)	12 ℃	14 ℃	16 ℃	18 ℃	20 ℃
8 h/16 h	8.00±0.58 ABc	23.33±0.33 Cb	24.00±1.15 Db	23.00±0.57 Eb	27.00±1.15 Da
10 h/14 h	8.67±0.33 ABe	24.00±1.15 Cd	26.00±1.15 Ab	25.33±0.33 Cc	27.33±0.33 Ca
12 h/12 h	10.00±0.67 Ad	22.33±0.33 Cc	24.67±0.33 Cb	25.00±0.57 Db	27.67±0.67 Ba
14 h/10 h	8.67±0.67 ABd	26.67±0.67 Ab	25.33±0.33 Bc	27.67±0.67 Aa	26.67±0.67 Eb
16 h/8 h	7.33±0.33 Bd	25.33±0.33 Bc	26.00±1.15 Ab	26.00±1.15 Bb	28.33±0.33 Aa
光周期 (光照/黑暗)	羽化率/%
光周期 (光照/黑暗)	12 ℃	14 ℃	16 ℃	18 ℃	20 ℃
8 h/16 h	33.27±10.39 Dd	4.29±0.06 De	54.19±0.20 Dc	65.17±0.88 Eb	83.98±0.70 Ca
10 h/14 h	46.30±1.85 Cd	11.04±1.01 Ce	64.06±0.84 Cc	67.13±0.87 Db	86.60±1.16 Ca
12 h/12 h	53.37±1.71 Bd	28.33±1.05 Be	71.61±0.39 Bc	78.70±0.92 Bb	92.76±0.17 Ba
14 h/10 h	61.67±0.83 Ac	30.04±0.73 Bd	72.36±0.36 Bb	73.52±0.55 Cb	100.00±0 Aa
16 h/8 h	58.93±1.79 Bd	35.54±0.46 Ae	76.83±1.03 Ac	88.42±0.52 Ab	96.47±0.04 ABa

时间/d	活蛹数
时间/d	处理	对照
2	29.00 ± 0.58 a		29.33 ± 0.67 a
4	27.00 ± 0.58 a		28.33 ± 0.88 a
6	26.67 ± 0.67 a		27.33 ± 0.33 a
8	17.33 ± 1.20 b		27.00 ± 0.58 a
10	14.00 ± 1.00 b		25.67 ± 1.33 a
时间/d	羽化率/%
时间/d	处理	对照
2	89.65 ± 0.21 b		96.59 ± 0.08 a
4	88.88 ± 0.24 b		96.46 ± 0.11 a
6	3.75 ± 0.09 b		100.00 ± 0 a
8	5.83 ± 0.43 b		100.00 ± 0 a
10	0 ± 0 b			100.00 ± 0 a

时间/d	活蛹数
时间/d	处理	对照
2	29.00 ± 0.58 a		29.33 ± 0.67 a
4	27.00 ± 0.58 a		28.33 ± 0.88 a
6	26.67 ± 0.67 a		27.33 ± 0.33 a
8	17.33 ± 1.20 b		27.00 ± 0.58 a
10	14.00 ± 1.00 b		25.67 ± 1.33 a
时间/d	羽化率/%
时间/d	处理	对照
2	89.65 ± 0.21 b		96.59 ± 0.08 a
4	88.88 ± 0.24 b		96.46 ± 0.11 a
6	3.75 ± 0.09 b		100.00 ± 0 a
8	5.83 ± 0.43 b		100.00 ± 0 a
10	0 ± 0 b			100.00 ± 0 a

贮藏时间/ d	羽化率/ %	雌蜂寿命/ d	产卵期/ d	寄生率/ %
40	86.67 ± 1.76 a	26.03 ± 0.24 b	9.85 ± 0.23 a	66.97 ± 0.61 b
80	81.33 ± 1.76 ab	28.26 ± 0.20 a	7.93 ± 0.20 b	62.19 ± 0.68 c
120	74.00 ± 2.31 bc	9.90 ± 0.25 c	3.58 ± 0.10 c	27.69 ± 0.83 d
160	62.00 ± 2.31 d	8.16 ± 0.24 d	2.04 ± 0.10 d	21.15 ± 0.41 e
200	54.00 ± 2.31 e	6.99 ± 0.12 e	1.99 ± 0.10 d	10.91 ± 0.51 f
240	48.00 ± 2.31 ef	5.34 ± 0.18 f	1.97 ± 0.10 d	9.13 ± 0.27 fg
280	45.00 ± 0.58 f	2.72 ± 0.13 g	1.34 ± 0.06 e	9.34 ± 0.27 f
300	20.00 ± 1.15 g	1.91 ± 0.15 g	1.18 ± 0.07 e	6.57 ± 0.42 gh
对照	80.00 ± 3.46 b	26.22 ± 0.27 b	10.02 ± 0.24 a	68.59 ± 0.65 a