Mitigative effect of exogenous choline chloride on wheat seedlings under drought stress

doi:10.16178/j.issn.0528-9017.20240670

Abstract

Abstract:

In order to investigate the effects of exogenous choline chloride on the growth and physiological characteristics of wheat seedlings under drought stress, Huaimai 35 and Zhengmai 9023 were selected as materials. The pot experiment method was adopted, and a 15% PEG-6000 solution was regularly irrigated to simulate a drought environment. 400 mg·L^-1 choline chloride was sprayed on the leaves to treat the wheat seedlings. The alleviating effects of choline chloride on the growth and physiological characteristics of wheat seedlings under drought stress were analyzed. The results showed that under drought stress, the growth and development of wheat seedlings were inhibited. There were significant decreases in the number of tillers, leaves, roots, the longest root length and water content, and the root-shoot ratio significantly increased. Meanwhile, the activities of superoxide dismutase (SOD), peroxidase (POD), as well as the contents of proline and malondialdehyde (MDA), were significantly increased. After treatment with 400 mg·L^-1 choline chloride, the activities of SOD, POD, and catalase (CAT) in wheat seedlings under drought stress were significantly increased, the scavenging ability of reactive oxygen species was greatly enhanced. Meanwhile, the content of MDA was significantly reduced, the content of proline was significantly increased, and the osmotic regulation ability of plants was strengthened. In terms of external morphology, it was manifested as a significant increase in the number of tillers, leaves, the longest root length, water content and a significant decrease in root-shoot ratio. The above results indicated that choline chloride can effectively alleviate the adverse effects of drought stress on the growth of wheat seedlings.

Key words: wheat seedling, drought stress, choline chloride, morphological indicator, physiological indicator

CLC Number:

S512.1

ZHANG Jinrui, LIU Zeliang, LUO Lei, PU Kairui, WANG Fei, JIANG Zihang, WANG Huadun, CHEN Xinhong, ZHANG Linqing. Mitigative effect of exogenous choline chloride on wheat seedlings under drought stress[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(1): 18-25.

Figures/Tables 7

References 25

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品种	处理	单株分蘖数	单株叶数	单株根数	最长根长/cm
淮麦35	CK0	2.14±0.23 b	7.45±0.58 a	7.67±1.06 a	11.96±2.99 a
	DS0	1.48±0.22 d	5.39±0.53 c	5.79±0.74 b	10.76±1.55 c
	CK400	2.77±0.35 a	7.97±0.45 a	7.82±1.02 a	13.82±1.33 a
	DS400	1.90±0.22 c	6.39±0.36 b	6.96±0.97 b	11.37±1.03 b
郑麦9023	CK0	2.16±0.24 b	5.85±0.59 a	7.84±1.07 a	12.05±1.89 a
	DS0	1.43±0.23 d	3.78±0.54 c	6.03±0.75 b	10.87±1.44 c
	CK400	2.44±0.36 a	6.86±0.46 a	7.92±1.02 a	13.91±1.28 a
	DS400	1.89±0.20 c	5.28±0.37 b	7.05±0.87 b	11.43±1.02 b

品种	处理	单株分蘖数	单株叶数	单株根数	最长根长/cm
淮麦35	CK0	2.14±0.23 b	7.45±0.58 a	7.67±1.06 a	11.96±2.99 a
	DS0	1.48±0.22 d	5.39±0.53 c	5.79±0.74 b	10.76±1.55 c
	CK400	2.77±0.35 a	7.97±0.45 a	7.82±1.02 a	13.82±1.33 a
	DS400	1.90±0.22 c	6.39±0.36 b	6.96±0.97 b	11.37±1.03 b
郑麦9023	CK0	2.16±0.24 b	5.85±0.59 a	7.84±1.07 a	12.05±1.89 a
	DS0	1.43±0.23 d	3.78±0.54 c	6.03±0.75 b	10.87±1.44 c
	CK400	2.44±0.36 a	6.86±0.46 a	7.92±1.02 a	13.91±1.28 a
	DS400	1.89±0.20 c	5.28±0.37 b	7.05±0.87 b	11.43±1.02 b

品种	处理	根冠比	含水量/%
淮麦35	CK0	0.09±0.01 c	88.44±0.32 a
	DS0	0.21±0.02 a	69.10±0.35 c
	CK400	0.12±0.01 b	89.66±1.21 a
	DS400	0.14±0.02 b	75.62±2.13 b
郑麦9023	CK0	0.11±0.01 c	89.13±0.35 a
	DS0	0.22±0.02 a	71.19±0.51 c
	CK400	0.14±0.01 b	89.85±1.17 a
	DS400	0.17±0.02 b	82.33±2.09 b

品种	处理	根冠比	含水量/%
淮麦35	CK0	0.09±0.01 c	88.44±0.32 a
	DS0	0.21±0.02 a	69.10±0.35 c
	CK400	0.12±0.01 b	89.66±1.21 a
	DS400	0.14±0.02 b	75.62±2.13 b
郑麦9023	CK0	0.11±0.01 c	89.13±0.35 a
	DS0	0.22±0.02 a	71.19±0.51 c
	CK400	0.14±0.01 b	89.85±1.17 a
	DS400	0.17±0.02 b	82.33±2.09 b

品种	处理	超氧化物歧化酶 (SOD)活性	过氧化物酶 (POD)活性	过氧化氢酶 (CAT)活性
淮麦35	CK0	1 164.88±6.21 d	216.96±7.21 d	16.16±2.27 b
	DS0	1 418.25±3.20 c	447.42±5.97 b	24.61±1.81 b
	CK400	1 571.68±3.80 b	309.72±2.60 c	48.67±4.06 a
	DS400	1 734.50±17.12 a	518.04±7.24 a	55.12±1.87 a
郑麦9023	CK0	1 186.03±9.07 d	184.74±2.76 d	16.51±0.01 b
	DS0	1 419.12±3.86 c	286.63±1.71 c	17.69±0.01 b
	CK400	1 518.35±19.36 b	383.38±1.06 b	45.29±2.01 a
	DS400	1 601.85±12.89 a	455.24±2.67 a	46.64±2.07 a