Abstract:

In order to explore the differences in volatile components of different types of tobacco extracts，headspace solid-phase microextraction gas chromatography-mass spectrometry（HS-SPME-GC-MS）was used to analyze the volatile components in three types of extracts：burley tobacco，spice tobacco，and Yuyan tobacco. The key differential volatile components were screened by orthogonal partial least squares discriminant analysis （OPLS-DA）. The results showed that a total of 111 volatile components were detected in the three tobacco extracts，with heterocyclic，hydrocarbon，and ketone compounds accounting for over 90% of the total content. There were 33 volatile components in three types of tobacco extracts，and there were significant differences in the types and contents of volatile components in different types of tobacco extracts. The OPLS-DA results indicated that benzyl alcohol，phenylacetaldehyde，α-damascenone，solanone，4，7，9-megastigmatrien-3-one B，4，7，9-megastigmatrien-3-one D，and neophytadiene were key differential volatile components in different types of tobacco extracts，which can be used to distinguish different types of tobacco extracts to some extent. This study can provide a theoretical reference for optimizing the aroma characteristics of different types of tobacco extracts.

Key words: tobacco extract, volatile component, orthogonal partial least squares discriminant analysis（OPLS-DA）