Identification，cultivation characteristics of an isolated wild Taiwanofungus camphoratus strain and the capacity of producing asexual spores

doi:10.16178/j.issn.0528-9017.20250378

Journal of Zhejiang Agricultural Sciences ›› 2026, Vol. 67 ›› Issue (7): 1783-1788.DOI: 10.16178/j.issn.0528-9017.20250378

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Identification，cultivation characteristics of an isolated wild Taiwanofungus camphoratus strain and the capacity of producing asexual spores

ZHANG Lu¹^,²(), KE Binrong³, ZHENG Shizhong¹^,⁴, WEI Qi⁵, ZHANG Weirui¹^,⁴, LIU Shengrong¹^,⁴()

^1.College of Biological Science and Engineering，Ningde Normal University，Ningde 352100，Fujian
^2.College of Life Science，Fujian Agriculture and Forestry University，Fuzhou 350002，Fujian
^3.Institute of Edible Fungi，Fujian Academy of Agricultural Sciences，Fuzhou 350011，Fujian
^4.Fujian Provincial University Engineering Research Center for Characteristic Biological Resources of Eastern Fujian，Ningde 352100，Fujian
^5.College of Ocean，Ningde Normal University，Ningde 352100，Fujian

Received:2025-05-22 Online:2026-07-11 Published:2026-07-27
Contact: LIU Shengrong

Abstract

Abstract:

Taiwanofungus camphoratus is a precious medicinal fungus endemic in Taiwan，China. This study identified an isolated wild Taiwanofungus camphoratus strain and examined its mycelial growth characteristics under liquid culture conditions. Morphological and ITS（Internal Transcribed Spacer）phylogenetic analysis identified the strain Ac-NF2 as Taiwanofungus camphoratus. For strain Ac-NF2，the suitable single carbon source in liquid culture was maltose，while the optimal combined carbon source was a combination of glucose and maltose. The appropriate single nitrogen source was yeast extract，and the preferred combined nitrogen source was a combination of peptone and yeast extract. The optimal pH value of the medium was between 5 and 6，and the temperature range of 25 and 27 ℃ was desirable for the mycelial growth. The strain produced easily asexual spores in liquid cultures，and the density of asexual spores reached 3.2×10⁷ mL^-1. The present study not only broadened the germplasm source of Taiwanofungus camphoratus，but also provided reference for the application of this strain in liquid fermentation production of mycelium and active ingredients.

Key words: Taiwanofungus camphoratus, strain identification, carbon source, nitrogen source, asexual spore

CLC Number:

S567.3

ZHANG Lu, KE Binrong, ZHENG Shizhong, WEI Qi, ZHANG Weirui, LIU Shengrong. Identification，cultivation characteristics of an isolated wild Taiwanofungus camphoratus strain and the capacity of producing asexual spores[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(7): 1783-1788.

Figures/Tables 5

Fig.1 Mycelial growth of Taiwanofungus camphoratus strain Ac-NF2 on inclined plane（A）and flat plate（B）

Fig. 2 Phylogenetic tree of the ITS sequences of the Taiwanofungus camphoratus strain Ac-NF2 and its reference strains

Fig. 3 Mycelial growth of Taiwanofungus camphoratus strain Ac-NF2 under different carbon and nitrogen sources

Fig. 4 Mycelial growth of Taiwanofungus camphoratus strain Ac-NF2 under different temperature and pH value

Fig.5 Asexual spores produced by Taiwanofungus camphoratus strain Ac-NF2 in liquid culture condition

References 18

[1]	Lu M C， El-Shazly M， Wu T Y，et al. Recent research and development of Antrodia cinnamomea ［J］. Pharmacology Therapeutics，2013，139（2）：124-156.
[2]	Geethangili M， Tzeng Y M. Review of pharmacological effects of Antrodia camphorata and its bioactive compounds［J］. Evidence-Based Complementary and Alternative Medicine，2011，2011：212641.
[3]	Zhang B B， Guan Y Y， Hu P F，et al. Production of bioactive metabolites by submerged fermentation of the medicinal mushroom Antrodia cinnamomea：recent advances and future development［J］. Critical Reviews in Biotechnology，2019，39（4）：541-554.
[4]	田雪梅，张国利，宋爱荣. 液体发酵法筛选樟芝优良诱变新菌株的研究［J］. 菌物学报，2012，31（6）：900-908.
[5]	周会明，张焱珍，柴红梅，等. 一株野生花脸香蘑的生物学特性及其栽培［J］. 食用菌学报，2017，24（1）：39-44.
[6]	Shih I L， Pan K， Hsieh C. Influence of nutritional components and oxygen supply on the mycelial growth and bioactive metabolites production in submerged culture of Antrodia cinnamomea ［J］. Process Biochemistry，2006，41（5）：1129-1135.
[7]	Lin E S， Chen Y H. Factors affecting mycelial biomass and exopolysaccharide production in submerged cultivation of Antrodia cinnamomea using complex media［J］. Bioresource Technology，2007，98（13）：2511-2517.
[8]	Lin E S， Sung S C. Cultivating conditions influence exopolysaccharide production by the edible Basidiomycete Antrodia cinnamomea in submerged culture［J］. International Journal of Food Microbiology，2006，108（2）：182-187.
[9]	Elisashvili V. Submerged cultivation of medicinal mushrooms：bioprocesses and products（review）［J］. International Journal of Medicinal Mushrooms，2012，14（3）：211-239.
[10]	Dudekula U T， Doriya K， Devarai S K. A critical review on submerged production of mushroom and their bioactive metabolites［J］. 3Biotech，2020，10（8）：337.
[11]	凌庆枝，刘国庆，袁怀波，等. pH和无机盐对樟芝液体发酵的影响［J］. 食品科学，2007，28（11）：365-369.
[12]	胡晓强，李峰，赵建选，等. 1株野生平菇菌株的采集及其驯化栽培［J］. 中国食用菌，2014，33（6）：13-14，16.
[13]	白雪，王玲芝，余锦欣，等. 牛樟芝的分离鉴定及最适谷物皿培基质的筛选［J］. 福建农业科技，2022，53（11）：10-16.
[14]	Yang F C， Yang Y H， Lu H C. Enhanced antioxidant and antitumor activities of Antrodia cinnamomea cultured with cereal substrates in solid state fermentation［J］. Biochemical Engineering Journal，2013，78：108-113.
[15]	胡鹏飞，陈磊，许赣荣，等. 以小米为基质牛樟芝固态发酵高产antroquinonol的条件优化［J］. 食品与发酵工业，2019，45（9）：137-144.
[16]	Pan J H， Chen Y S， Sheen L Y，et al. Large-scale submerged fermentation of Antrodia cinnamomea for anti-hepatoma activity［J］. Journal of the Science of Food and Agriculture，2008，88（13）：2223-2230.
[17]	Zhang H， Hu Y D， Lu R Q，et al. Integrated strategy of pH-shift and glucose feeding for enhanced production of bioactive antrodin C in submerged fermentation of Antrodia camphorata ［J］. Journal of Industrial Microbiology Biotechnology，2014，41（8）：1305-1310.
[18]	陈泰伊，仲伟鉴，肖萍，等. 樟芝菌丝体发酵全液冻干粉长期食用安全性评估［J］. 食用菌学报，2012，19（2）：100-105.

Identification，cultivation characteristics of an isolated wild Taiwanofungus camphoratus strain and the capacity of producing asexual spores

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