AMETH_3452基因在拟无枝酸菌中的系统发育分析

doi:10.16178/j.issn.0528-9017.20210437

浙江农业科学 ›› 2021, Vol. 62 ›› Issue (4): 763-767.DOI: 10.16178/j.issn.0528-9017.20210437

AMETH_3452基因在拟无枝酸菌中的系统发育分析

唐标¹(), 吴静¹, 郑华军², 赵维³, 杨华¹^,^*()

1.浙江省农业科学院农产品质量安全与营养研究所,浙江杭州 310021
2.上海人类基因组研究中心,上海 201203
3.中国科学院深圳先进技术研究院,广东深圳 518055

收稿日期:2020-12-24 出版日期:2021-04-11 发布日期:2021-04-02
通讯作者: 杨华
作者简介:杨华(1972—),男,浙江绍兴人,研究员,博士,主要从事动物源细菌耐药性研究工作,E-mail: yanghua@zaas.ac.cn。
唐标(1987—),男,江苏徐州人,助理研究员,博士,从事基因组学与动物源细菌耐药性研究工作,E-mail: tb_411@163.com。
基金资助:
国家自然科学基金青年项目(31700007);浙江省重点研发计划(2020C02031)

Received:2020-12-24 Online:2021-04-11 Published:2021-04-02

摘要/Abstract

摘要：

AMETH_3452基因是拟无枝酸菌(Amycolatopsis)中发现的一个放线菌的保守基因,位于拟无枝酸菌属中的环形基因组中间位置。为进一步探究该基因的序列特征并挖掘该基因的应用潜力,本研究将该基因构建在pET28载体上,在大肠杆菌中进行表达,使用蛋白预测网站进行了二级结构和保守区分析,对其直系同源基因在拟无枝酸菌属进行了系统发育分析。AMETH_3452蛋白在大肠杆菌的上清液中可溶性表达。该基因在拟无枝酸菌属的核酸同源性为79.23%~99.02%,在300~500 bp的序列一致性较高。该蛋白序列共269个氨基酸,作为分子标记在76个拟无枝酸菌中进行系统发育分析后发现,东方拟无枝酸菌分支和甲基拟无枝酸菌分支稳定性高,同时可将东方拟无枝酸菌分支分成AORIS和AMEDS 2个可稳定的分支。以上研究将为AMETH_3452基因作为检测环境、动物或临床上的放线菌或系统发育应用研究提供依据。

中图分类号:

Q939.96

唐标, 吴静, 郑华军, 赵维, 杨华. AMETH_3452基因在拟无枝酸菌中的系统发育分析[J]. 浙江农业科学, 2021, 62(4): 763-767.

图/表 3

参考文献 21

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AMETH_3452基因在拟无枝酸菌中的系统发育分析

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