小田块中稻瘟菌营养体亲和群复杂性研究

doi:10.16178/j.issn.0528-9017.20230955

摘要/Abstract

摘要：

2021年9月采集稻瘟病穗颈瘟样本,分离得到46株稻瘟菌单分生孢子菌株,并通过配对培养法分析不同稻瘟菌菌株间的营养体亲和性情况。研究结果表明,在马铃薯葡萄糖琼脂培养基上,稻瘟菌菌株间的亲和性配对培养结果可明显区分为营养体亲和(vegetative compatible, VC)与不亲和(vegetative incompatible, VIC)两种反应类型,其中发生VC反应的菌株间的菌落交界处没有明显的抗衡带,而发生VIC反应的菌株间的菌落交界处则出现明显的白色抗衡带,且有部分菌株沿白色抗衡带边缘出现色素加深的现象。通过对46株不同单孢菌株间的亲和性测定结果所进行的统计分析表明,配对菌株间发生不亲和反应的比例占配对组合总数的77.3%,根据真菌营养体亲和群(vegetative compatibility group, VCG)的定义,可将46个单孢菌株中的20个归入16个不同的VCG之中,另有26个菌株因为能够与多个VCG内的全部或部分成员亲和,不能划归任何VCG。研究结果提示,在小田块中,稻瘟菌群体有非常复杂的VCG结构,不同VCG菌株间的VIC反应可能会严重限制真菌病毒的水平传播并影响弱毒相关真菌病毒对稻瘟病的生防效率。

关键词: 稻瘟菌, 真菌病毒, 弱毒力, 营养体亲和群(VCG)

中图分类号:

S435.11

王谣, 丁航, 李聪, 邓清超. 小田块中稻瘟菌营养体亲和群复杂性研究[J]. 浙江农业科学, 2023, 64(12): 2939-2943.

图/表 3

图1 稻瘟病菌的营养体亲和性反应类型 A为亲和性反应型Ⅰ,A1、A2、A3分别为对峙培养的培养皿的正反两面,以及透光条件下的反面;B为亲和性反应Ⅱ型,B1、B2、B3分别为对峙培养的培养皿的正反面,以及透光条件下的反面;C为亲和性反应型Ⅲ型,C1、C2、C3分别为对峙培养的培养皿的正反面,以及透光条件下的反面。

表1 参试稻瘟菌株的VCG分析

亲和群	菌株编号	亲和群	菌株编号
1	ES31, ES45	9	ES62,ES51
2	ES34	10	ES58
3	ES50,ES39	11	ES61
4	ES42	12	ES63
5	ES43	13	ES64
6	ES46	14	ES71, ES65
7	ES48	15	ES74
8	ES49	16	ES75

表2 与多个VCG的全部或部分成员亲和的菌株

亲和的VCG数量
2	3	4	5	6	7
ES32	ES30	ES33	ES40		ES44
ES37	ES35	ES55	ES66
ES38	ES36	ES67
ES53	ES41	ES69
ES54	ES47
ES56	ES52
ES57	ES70
ES59	ES72
ES60	ES73
ES68

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