光唇鱼植入式可视荧光标记应用评估

doi:10.16178/j.issn.0528-9017.20240502

摘要/Abstract

摘要：

为建立一种便捷高效的光唇鱼(Acrossocheilus fasciatus)标记技术,以服务其育种实践和增殖放流效果评估,本试验利用植入式可视荧光标记(VIE),开展了不同颜色荧光标记和不同规格鱼种的短期标记效果对比试验,并评估了标记对光唇鱼短期生长的影响。结果显示,全长分别为(4.43±0.13)cm和(6.32±0.15)cm的幼鱼,在标记30 d后存活率均大于95%(分别为98.0%和99.5%),红色和绿色标记的保持率均为100%,其中,红色荧光标记更加清晰,更易于肉眼识别。在相同养殖条件下,对规格为(4.43±0.13)cm的幼鱼进行红色荧光标记,30 d后其存活率、标记保持率和特定生长率(SGR)分别为96.50%、100%和(1.17±0.16)%·d^-1,且SGR与对照组无显著差异(p>0.05)。本试验证实了荧光标记法在光唇鱼幼鱼个体标记中的有效性及适用性,表明该技术对苗种生长和存活无负面影响。

关键词: 光唇鱼, 植入式可视荧光标记, 增殖放流, 存活率, 标记保持率

Abstract:

In order to establish a convenient and efficient tagging technique for Acrossocheilus fasciatus to support its breeding practice and evaluate the enhancement and releasing effects, this study employed visible implant elastomer (VIE) to conduct short-term comparative trials on the effects of different VIE colors and fish sizes. The impact of tagging on the short-term growth of juvenile fish was also assessed. The results showed that the 30-day survival rates of juveniles with total lengths of (4.43±0.13) cm and (6.32±0.15) cm both exceeded 95% (98.0% and 99.5%, respectively), and the tag retention rates in both groups were 100%. However, the red VIE tags were clearer and easier to recognize with the naked eye than the green tags. Under the same rearing conditions, the 30-day survival rate, tag retention rate, and specific growth rate (SGR) of the red VIE-tagged group [with a total length of (4.43±0.13) cm] were 96.50%, 100%, and (1.17±0.16)%·d^-1, respectively. No significant difference in SGR was observed between the tagging group and the control group (p>0.05). This study confirms that VIE tagging is an effective and applicable method for individual marking of A. fasciatus juveniles, with no adverse effects on their growth or survival.

Key words: Acrossocheilus fasciatus, visible implant elastomer, enhancement and releasing, survival rate, tag retention rate

中图分类号:

S917

简杰亮, 范峰林, 王宝莲, 吴旭国, 许晓军. 光唇鱼植入式可视荧光标记应用评估[J]. 浙江农业科学, 2026, 67(1): 161-165.

JIAN Jieliang, FAN Fenglin, WANG Baolian, WU Xuguo, XU Xiaojun. Evaluation of visible implant elastomer tagging for Acrossocheilus fasciatus[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(1): 161-165.

图/表 4

图1 光唇鱼幼鱼荧光标记位置

Fig.1 Fluorescent marking location in juvenile of Acrossocheilus fasciatus

图2 不同规格的光唇鱼幼鱼照片

Fig.2 Photos of different sized juvenile of Acrossocheilus fasciatus

表1 不同规格光唇鱼的幼鱼存活率和标记保持率

Table 1 Survival rate and tag retention rate of different sized juvenile of Acrossocheilus fasciatus

标记颜色	起始规格/cm	存活率/%	标记保持率/%
红色	4.43±0.13	98.0	100
绿色	6.32±0.15	99.5	100

表2 荧光标记对光唇鱼幼鱼生长的影响

Table 2 Effects of visible implant elastomer tagging on the growth of juvenile of A. fasciatus

处理	初始体重/g	终末体重/g	特定生长率/(%·d^-1)	标记保持率/%		存活率/%
荧光标记组	0.90±0.15 a	1.28±0.15 a	1.17±0.16 a	100	96.50±1.00 a
对照组	0.92±0.16 a	1.26±0.16 a	1.05±0.07 a	—	96.67±1.15 a

参考文献 31

[1]	袁乐洋. 中国光唇鱼属鱼类的分类整理[D]. 南昌: 南昌大学, 2005.
	YUAN L Y. Taxonomic revision of Chinese species of the cyprinid genus Acrossocheilus (Teleostei: Cypriniformes)[D]. Nanchang: Nanchang University, 2005.
[2]	周华兴, 胡玉婷, 段国庆, 等. 基于线粒体细胞色素b基因序列的新安江流域温州光唇鱼群体遗传研究[J]. 渔业科学进展, 2019, 40(2): 43-50.
	ZHOU H X, HU Y T, DUAN G Q, et al. Population genetic analyses of Acrossocheilus wenchowensis in Xin'an Basin based on the mitochondrial DNA cytochrome b gene[J]. Progress in Fishery Sciences, 2019, 40(2): 43-50.
[3]	冀德伟, 李明云, 史雨红, 等. 光唇鱼的肌肉营养组成与评价[J]. 营养学报, 2009, 31(3): 298-300.
	JI D W, LI M Y, SHI Y H, et al. The nutritional composition and evaluation in muscle of Acrossocheilus fasciatus[J]. Acta Nutrimenta Sinica, 2009, 31(3): 298-300.
[4]	王艳艳, 詹会祥, 李正友, 等. 野生云南光唇鱼肌肉营养成分分析及品质评价[J]. 南方农业学报, 2017, 48(3): 507-511.
	WANG Y Y, ZHAN H X, LI Z Y, et al. Nutritional components analysis and nutritive value evaluation of wild Acrossocheilus yunnanensis muscle[J]. Journal of Southern Agriculture, 2017, 48(3): 507-511.
[5]	马旭东. 被遗忘在饲料鱼中的中国观赏鱼资源[J]. 水族世界, 2007(2): 64-68.
	MA X D. The forgotten Chinese ornamental fish resources among forage fish[J]. Fish World, 2007(2): 64-68.
[6]	夏继红, 秦如照, 窦传彬, 等. 中小河流鱼类生境适宜性评估模型与等级分区[J]. 水利水电科技进展, 2022, 42(3): 9-13, 31.
	XIA J H, QIN R Z, DOU C B, et al. Habitat suitability assessment model and suitability grading classification for fishes in small and medium rivers[J]. Advances in Science and Technology of Water Resources, 2022, 42(3): 9-13, 31.
[7]	马建波, 朱卫东, 申屠琰, 等. 光唇鱼苗种繁育技术[J]. 科学养鱼, 2020(6): 7.
	MA J B, ZHU W D, SHENTU Y, et al. Breeding technology of Acrossocheilus fasciatus[J]. Scientific Fish Farming, 2020(6): 7.
[8]	黄雅贞, 叶赣明, 刘德亭, 等. 光唇鱼仿生态繁育与健康养殖技术[J]. 中国水产, 2022(3): 86-89.
	HUANG Y Z, YE G M, LIU D T, et al. Eco-simulated breeding and healthy aquaculture techniques for Acrossocheilus fasciatus[J]. China Fisheries, 2022(3): 86-89.
[9]	周华书. 光唇鱼水泥池工厂化养殖技术[J]. 科学养鱼, 2019(12): 33-35.
	ZHOU H S. Intensive aquaculture technology for Acrossocheilus fasciatus in concrete tanks[J]. Scientific Fish Farming, 2019(12): 33-35.
[10]	程家骅, 姜亚洲. 海洋生物资源增殖放流回顾与展望[J]. 中国水产科学, 2010, 17(3): 610-617.
	CHENG J H, JIANG Y Z. Marine stock enhancement: review and prospect[J]. Journal of Fishery Sciences of China, 2010, 17(3): 610-617.
[11]	郝雅宾, 原居林, 刘金殿, 等. 黄尾鲴和花䱻增殖放流标记技术研究[J]. 江苏农业科学, 2018, 46(22): 160-164.
	HAO Y B, YUAN J L, LIU J D, et al. Study on tagging techniques for release and enhancement of Xenocypris davidi and Hemibarbus maculatus[J]. Jiangsu Agricultural Sciences, 2018, 46(22): 160-164.
[12]	DOUPÉ R G, PARTRIDGE G J, LYMBERY A J. Visible implant fluorescent elastomer tags as pedigree markers for applied aquaculture: an evaluation using black bream Acanthopagrus butcheri[J]. Aquaculture Research, 2003, 34(8): 681-683.
[13]	BRENNAN N P, LEBER K M, BLACKBURN B R. Use of coded-wire and visible implant elastomer tags for marine stock enhancement with juvenile red snapper Lutjanus campechanus[J]. Fisheries Research, 2007, 83(1): 90-97.
[14]	ZHU T B, GUO W, WU X B, et al. Effects of visible implant elastomer and coded wire tags on growth and survival of Schizopygopsis younghusbandi Regan, 1905[J]. Journal of Applied Ichthyology, 2016, 32(1): 110-112.
[15]	KAPUSTA A, DUDA A, WISZNIEWSKI G, et al. Preliminary evaluation of the effectiveness of visible implant elastomer and coded wire tags for tagging young-of-the-year Atlantic sturgeon, Acipenser oxyrinchus[J]. Archives of Polish Fisheries, 2015, 23(4): 227-230.
[16]	杨晓鸽, 危起伟, 杜浩, 等. 可见植入荧光标记和编码金属标对达乌尔鳇标志效果的初步研究[J]. 淡水渔业, 2013, 43(2): 43-47.
	YANG X G, WEI Q W, DU H, et al. Primary study on tag performance of Kaluga sturgeon(Huso dauricus) tagged with visible implant elastomer and coded wire tag[J]. Freshwater Fisheries, 2013, 43(2): 43-47.
[17]	陈雷, 刘海映, 王秀利, 等. 荧光标记红鳍东方鲀可行性研究[J]. 生物技术通报, 2014, 30(2): 112-115.
	CHEN L, LIU H Y, WANG X L, et al. Feasibility of fluorescent tag on Takifugu rubripes[J]. Biotechnology Bulletin, 2014, 30(2): 112-115.
[18]	李晶晶, 耿绪云, 董学旺, 等. 中华绒螯蟹可视荧光标记应用研究[J]. 海洋渔业, 2016, 38(4): 400-405.
	LI J J, GENG X Y, DONG X W, et al. Application research of visible fluorescent elastomer tagging in the Chinese mitten crab (Eriocheir sinensis)[J]. Marine Fisheries, 2016, 38(4): 400-405.
[19]	陈锦淘, 戴小杰. 鱼类标志放流技术的研究现状[J]. 上海水产大学学报, 2005, 14(4): 4451-4456.
	CHEN J T, DAI X J. Current status of fish tagging technology[J]. Journal of Shanghai Fisheries University, 2005, 14(4): 4451-4456.
[20]	赵萍, 王从锋, 刘德富, 等. 北盘江流域增殖放流鱼类的标志方法研究[J]. 水产学杂志, 2014, 27(2): 29-31,51.
	ZHAO P, WANG C F, LIU D F, et al. A study on tagging methods for fish stock enhancement in the Beipan River basin[J]. Chinese Journal of Fisheries, 2014, 27(2): 29-31,51.
[21]	李柯懋, 简生龙, 王国杰, 等. 应用于花斑裸鲤增殖放流效果评价的两种标记方法对比研究[J]. 淡水渔业, 2021, 51(5): 45-50.
	LI K M, JIAN S L, WANG G J, et al. Comparative study on two tagging methods applied to evaluate the effect of enhancement and release of Gymnocypris eckloni[J]. Freshwater Fisheries, 2021, 51(5): 45-50.
[22]	高保全, 刘萍, 李健, 等. 三疣梭子蟹的两种标记技术[J]. 渔业科学进展, 2013, 34(6): 87-93.
	GAO B Q, LIU P, LI J, et al. Two types of tagging techniques for Portunus trituberculatus[J]. Progress in Fishery Sciences, 2013, 34(6): 87-93.
[23]	IM J H, GIL H W, PARK I S, et al. Evaluation of visible fluorescent elastomer tags implanted in marine medaka, Oryzias dancena[J]. Fisheries and Aquatic Sciences, 2017, 20: 21.
[24]	刘海映, 王秀利, 田燚, 等. 成体对虾的荧光标记研究[J]. 生物技术通报, 2009, 25(4): 122-125.
	LIU H Y, WANG X L, TIAN Y, et al. Study of fluorescent tag on adult shrimp[J]. Biotechnology Bulletin, 2009, 25(4): 122-125.
[25]	REEVES K S, BUCKMEIER D L. Mortality, predation, and tag visibility of fish marked with visible implant elastomer tags[J]. North American Journal of Fisheries Management, 2009, 29(2): 323-329.
[26]	FREDERICK J. Evaluation of fluorescent elastomer injection as a method for marking small fish[J]. Bulletin of Marine Science, 1997, 61(2): 399-408.
[27]	NIELSEN L A. Methods of marking fish and shellfish[M]. Bethesda, Maryland: American Fisheries Society, 1992.
[28]	王茂元, 黄洪贵, 赖铭勇, 等. 鲢鱼增殖放流标志技术研究[J]. 江苏农业科学, 2015, 43(9): 261-263.
	WANG M Y, HUANG H G, LAI M Y, et al. A study on tagging technology for silver carp (Hypophthalmichthys molitrix) stock enhancement[J]. Jiangsu Agricultural Sciences, 2015, 43(9): 261-263.
[29]	PHINNEY D E, MATHEWS S B. Field test of fluorescent pigment marking and finclipping of coho salmon[J]. Journal of the Fisheries Research Board of Canada, 1969, 26(6): 1619-1624.
[30]	SZCZEPKOWSKI M, ZAKĘŚ Z, SZCZEPKOWSKA B, et al. Tag retention, growth rate, and survival of juvenile pike tagged with visible implant elastomer and coded wire tags[J]. Bulgarian Journal of Agricultural Science, 2015, 21(S1): 12-16.
[31]	SOULA M, NAVARRO A, HILDEBRANDT S, et al. Evaluation of VIE (Visible Implant Elastomer) and PIT (Passive Integrated Transponder) physical tagging systems for the identification of red porgy fingerlings (Pagrus pagrus)[J]. Aquaculture International, 2012, 20(3): 571-583.