浙江农业科学

浙江农业科学 ›› 2026, Vol. 67 ›› Issue (3): 600-607.DOI: 10.16178/j.issn.0528-9017.20240885

• 经济作物 • 上一篇    下一篇

烟叶醇化过程中的差异微生物蛋白质及其来源分析

张艺帆1(), 李锋2, 金立锋2, 杨月2, 附俊杰3, 王笑2, 许强1, 苗晨琳1, 陆震鸣3, 胡宗玉1()   

  1. 1.江苏中烟工业有限责任公司，江苏 南京 210015
    2.中国烟草总公司郑州烟草研究院，河南 郑州 450001
    3.江南大学 工业生物技术教育部重点实验室，江苏 无锡 214122
  • 收稿日期:2024-11-20 出版日期:2026-03-11 发布日期:2026-03-30
  • 通讯作者: 胡宗玉
  • 作者简介:胡宗玉，E-mail：huzy707@sina.com。
    张艺帆，研究方向为生物化学与分子生物学。E-mail：18305182402@163.com。
  • 基金资助:
    中国烟草总公司重点研发项目(110202102033)

Analysis of differential microbial proteins and their sources during the aging process of tobacco leaves

ZHANG Yifan1(), LI Feng2, JIN Lifeng2, YANG Yue2, FU Junjie3, WANG Xiao2, XU Qiang1, MIAO Chenlin1, LU Zhenming3, HU Zongyu1()   

  1. 1.China Tobacco Jiangsu Industrial Co. ，Ltd. ，Nanjing 210015，Jiangsu
    2.Zhengzhou Tobacco Research Institute of CNTC，Zhengzhou 450001，Henan
    3.Key Laboratory of Industrial Biotechnology of Ministry of Education，Jiangnan University，Wuxi 214122，Jiangsu
  • Received:2024-11-20 Online:2026-03-11 Published:2026-03-30
  • Contact: HU Zongyu

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摘要：

烟叶表面和内部的微生物种类丰富多样，与烟叶的醇化质量密切相关。本研究通过宏蛋白质组学技术解析了不同醇化时间烟叶的差异微生物蛋白质及其来源，以挖掘可以改善烟叶品质相关的酶，从而确定了关键功能微生物。结果表明，不同醇化时间的烟叶微生物蛋白质具有显著（p<0.05）差异。在烟叶醇化过程中，不动杆菌属（Acinetobacter）和鞘氨醇单胞菌属（Sphingomonas）能通过β-1，4-葡聚糖酶和β-葡萄糖苷酶协同分解纤维素；假单胞菌属（Pseudomonas）通过产生氨肽酶分解蛋白质；假单胞菌属和鞘氨醇单胞菌属可产生β-淀粉酶和葡糖淀粉酶协同降解淀粉。此外，假单胞菌属、不动杆菌属、肠杆菌属（Enterobacter）等优势菌属参与类胡萝卜素及其衍生物的降解。本研究为后续改良烟叶醇化策略提供了理论基础。

关键词: 烟叶醇化, 宏蛋白质组学, 差异微生物蛋白质, 功能微生物

Abstract:

The microorganisms on the surface and inside of tobacco leaves are rich and diverse，which are closely related to the aging quality of tobacco leaves. In this study，the differential microbial proteins and source microorganisms of tobacco leaves with different aging time were analyzed by macroproteomics technology，and the enzymes related to improving the quality of tobacco leaves were excavated to identify the key functional microorganisms. The results showed that the microbial proteins of tobacco leaves with different aging time were significantly （p<0.05） different. During the aging process of tobacco leaves，Acinetobacter and Sphingomonas synergistically degraded tobacco cellulose through β-1，4-glucanase and β-glucosidase. Pseudomonas facilitated protein degradation in tobacco leaves by producing aminopeptidase，while Pseudomonas and Sphingomonas produced β-amylase and glucoamylase to degrade starch synergistically. In addition，dominant genera such as Pseudomonas，Acinetobacter，and Enterobacter were involved in the degradation of carotenoids and their derivatives. This study provides a theoretical basis for the subsequent improvement of tobacco leaves aging strategies.

Key words: tobacco leaf aging, macroproteomic, differential microbial protein, functional microorganism

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