Effects of monthly average air temperature on photosynthetic characteristics and total flavonoids content in root tubers of Tetrastigma hemsleyanum Diels et Gilg seedlings grown in containers under the forest

doi:10.16178/j.issn.0528-9017.20230043

Abstract

Abstract:

The effects of monthly average temperature in Wufu Mountain from 2015 to 2019 on photosynthetic characteristics and total flavone content in root tubers of Tetrastigma hemsleyanum Diels et Gilg seedlings grown in containers under the forest were investigated. The results show that from 2015 to 2019, the temperature in Wufu Mountain is the lowest in January, rising gradually from February to June, reaching the highest value from July to August, and decreasing gradually from September to December. With the gradual increase of temperature from January to June, the SPAD value, net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r), stomatal limitation (L_s), instantaneous carboxylation rate (CUE), initial fluorescence (F_o), maximum fluorescence (F_m), minimum fluorescence (F'_o), maximum fluorescence under light (F'_m), steady-state fluorescence (F_s), maximum light energy conversion efficiency (F_v/F_m), potential photochemical efficiency of PSⅡ(F_v/F_o), actual photochemical efficiency of PSⅡ(Φ_PSⅡ), open PSⅡ reaction center capture excitation energy efficiency (F'_v/F'_m), photochemical quenching coefficient (q_P) and total flavone content in root tuber increased significantly(P<0.05), intercellular CO₂ concentration (C_i), water use efficiency (WUE) and non photochemical quenching coefficient (NPQ) decreased significantly(P<0.05). The temperature reached the highest value in July and August, and the chlorophyll content SPAD, P_n, G_s, T_r, L_s, CUE, F_o, F_m, F'_o, F'_m, F_s, F_v/F_m, F_v/F_o, ΦPSⅡ, F'_v/F'_m, q_P and the contents of total flavonoids in root tuber reached the maximum, and C_i, WUE and NPQ reached the minimum. From September to December, the temperature gradually decreased, SPAD, P_n, G_s, T_r, L_s, CUE, F_o, F_m, F'_o, F'_m, F_s, F_v/F_m, F_v/F_o,Φ_PSⅡ, F'_v/F'_m, q_P and the contents of total flavonoids in root tuber decreased significantly(P<0.05), while C_i, WUE and NPQ increased significantly(P<0.05). The results of this experiment can provide the basis of meteorological factors (air temperature) for container ecological cultivation of Tetrastigma hemsleyanum Diels et Gilg seedlings under the forest.

Key words: monthly average air temperature, Tetrastigma hemsleyanum Diels et Gilg, seedlings grown in containers under the forest, photosynthetic characteristics, root tuber, total flavone content

CLC Number:

S663

ZHANG Jianmin, LIN Guowei, LOU Jun. Effects of monthly average air temperature on photosynthetic characteristics and total flavonoids content in root tubers of Tetrastigma hemsleyanum Diels et Gilg seedlings grown in containers under the forest[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(3): 555-561.

Figures/Tables 4

References 36

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月份	平均气温/℃						叶绿素含量 (平均SPAD值)
月份	2015年	2016年	2017年	2018年	2019年	平均值	叶绿素含量 (平均SPAD值)
1	6.47	4.93	7.74	4.65	5.61	5.88 f	25.36 d
2	7.78	6.74	8.28	6.46	6.73	7.20 e	23.65 d
3	11.69	10.75	11.70	13.05	11.66	11.77 d	28.59 c
4	16.48	17.71	16.41	17.62	17.12	17.07 c	32.52 bc
5	20.69	20.17	21.13	22.64	19.68	20.86 b	36.98 b
6	23.96	17.90	22.83	23.56	23.13	22.28 b	35.87 b
7	23.69	22.99	26.81	26.55	25.24	25.06 a	48.39 a
8	24.12	21.83	26.01	26.16	26.22	24.87 a	49.68 a
9	20.99	18.49	24.17	22.81	22.68	21.83 b	34.27 b
10	17.90	20.33	17.69	15.68	18.81	18.08 c	33.32 bc
11	13.16	13.76	12.31	12.45	12.86	12.91 d	30.25 c
12	7.18	10.29	6.09	7.10	7.85	7.70 e	22.58 d

月份	平均气温/℃						叶绿素含量 (平均SPAD值)
月份	2015年	2016年	2017年	2018年	2019年	平均值	叶绿素含量 (平均SPAD值)
1	6.47	4.93	7.74	4.65	5.61	5.88 f	25.36 d
2	7.78	6.74	8.28	6.46	6.73	7.20 e	23.65 d
3	11.69	10.75	11.70	13.05	11.66	11.77 d	28.59 c
4	16.48	17.71	16.41	17.62	17.12	17.07 c	32.52 bc
5	20.69	20.17	21.13	22.64	19.68	20.86 b	36.98 b
6	23.96	17.90	22.83	23.56	23.13	22.28 b	35.87 b
7	23.69	22.99	26.81	26.55	25.24	25.06 a	48.39 a
8	24.12	21.83	26.01	26.16	26.22	24.87 a	49.68 a
9	20.99	18.49	24.17	22.81	22.68	21.83 b	34.27 b
10	17.90	20.33	17.69	15.68	18.81	18.08 c	33.32 bc
11	13.16	13.76	12.31	12.45	12.86	12.91 d	30.25 c
12	7.18	10.29	6.09	7.10	7.85	7.70 e	22.58 d

月份	平均气温/℃	P_n/ (μmol·m^-2·s^-1)	G_s/ (μmol·m^-2·s^-1)	C_i/ (μmol·mol^-1)	T_r/ (mmol·m^-2·s^-1)	L_s	WUE/ (μmol·mol^-1)	CUE/ (mmol·m^-2·s^-1)
1	5.88 f	1.06 e	0.01 e	125.02 a	0.19 d	0.69 c	5.58 a	0.008 5 d
2	7.20 e	1.03 e	0.01 e	110.26 b	0.23 d	0.72 bc	4.48 b	0.009 3 d
3	11.77 d	1.26 d	0.01 e	102.13 b	0.35 bc	0.74 bc	3.60 c	0.012 3 c
4	17.07 c	1.48 c	0.01 e	78.52 d	0.38 b	0.81 b	3.89 c	0.018 8 c
5	20.86 b	1.96 b	0.02 d	45.63 d	0.41 b	0.89 a	4.78 b	0.043 1 b
6	22.28 b	2.05 b	0.03 c	35.29 d	0.42 b	0.91 a	4.88 b	0.058 1 b
7	25.06 a	2.45 a	0.05 a	23.01 e	0.61 a	0.94 a	4.01 b	0.106 5 a
8	24.87 a	2.36 a	0.04 b	19.68 e	0.65 a	0.95 a	3.63 c	0.119 9 a
9	21.83 b	1.85 b	0.03 c	32.58 d	0.40 b	0.92 a	4.63 b	0.056 8 b
10	18.08 c	1.49 c	0.01 e	83.69 c	0.35 bc	0.79 b	4.26 b	0.017 8 c
11	12.91 d	1.28 d	0.01 e	128.42 a	0.31 c	0.68 c	4.13 b	0.009 9 d
12	7.70 e	1.21 d	0.01 e	132.54 a	0.21 d	0.67 c	5.76 a	0.009 1 d

月份	平均气温/℃	P_n/ (μmol·m^-2·s^-1)	G_s/ (μmol·m^-2·s^-1)	C_i/ (μmol·mol^-1)	T_r/ (mmol·m^-2·s^-1)	L_s	WUE/ (μmol·mol^-1)	CUE/ (mmol·m^-2·s^-1)
1	5.88 f	1.06 e	0.01 e	125.02 a	0.19 d	0.69 c	5.58 a	0.008 5 d
2	7.20 e	1.03 e	0.01 e	110.26 b	0.23 d	0.72 bc	4.48 b	0.009 3 d
3	11.77 d	1.26 d	0.01 e	102.13 b	0.35 bc	0.74 bc	3.60 c	0.012 3 c
4	17.07 c	1.48 c	0.01 e	78.52 d	0.38 b	0.81 b	3.89 c	0.018 8 c
5	20.86 b	1.96 b	0.02 d	45.63 d	0.41 b	0.89 a	4.78 b	0.043 1 b
6	22.28 b	2.05 b	0.03 c	35.29 d	0.42 b	0.91 a	4.88 b	0.058 1 b
7	25.06 a	2.45 a	0.05 a	23.01 e	0.61 a	0.94 a	4.01 b	0.106 5 a
8	24.87 a	2.36 a	0.04 b	19.68 e	0.65 a	0.95 a	3.63 c	0.119 9 a
9	21.83 b	1.85 b	0.03 c	32.58 d	0.40 b	0.92 a	4.63 b	0.056 8 b
10	18.08 c	1.49 c	0.01 e	83.69 c	0.35 bc	0.79 b	4.26 b	0.017 8 c
11	12.91 d	1.28 d	0.01 e	128.42 a	0.31 c	0.68 c	4.13 b	0.009 9 d
12	7.70 e	1.21 d	0.01 e	132.54 a	0.21 d	0.67 c	5.76 a	0.009 1 d

月份	平均气温/℃	F_o	F_m	F'_o	F'_m	F_s	F_v/F_m	F_v/F_o	Φ_PSⅡ	F'_v/F'_m	q_P	NPQ
1	5.88 f	216.3 g	1 346.5 f	423.5 f	1 235.4 f	983.5 c	0.56 b	1.51 c	0.21 d	0.34 d	0.51 c	0.74 a
2	7.20 e	326.6 f	1 645.2 e	502.3 e	1 756.2 e	995.2 c	0.58 b	1.58 c	0.25 d	0.32 d	0.52 c	0.72 a
3	11.77 d	485.9 e	1 752.2 e	598.6 d	2 459.2 d	989.6 c	0.59 b	2.06 b	0.32 c	0.44 c	0.66 b	0.59 b
4	17.07 c	563.5 d	2 155.9 d	612.6 d	2 865.2 c	998.4 c	0.63 b	2.15 b	0.35 c	0.45 c	0.65 b	0.61 b
5	20.86 b	665.8 c	2 569.2 c	685.5 c	3 205.6 b	1 002.3 c	0.75 a	2.46 b	0.46 b	0.59 b	0.78 a	0.41 c
6	22.28 b	723.4 b	3 168.2 b	736.2 b	3 215.2 b	1 235.6 b	0.78 a	3.54 a	0.52 b	0.61 b	0.79 a	0.42 c
7	25.06 a	836.9 a	3 725.5 a	823.6 a	3 795.6 a	1 523.6 a	0.73 a	3.68 a	0.64 a	0.73 a	0.80 a	0.31 d
8	24.87 a	852.5 a	3 659.2 a	863.3 a	3 815.2 a	1 623.8 a	0.79 a	3.75 a	0.69 a	0.78 a	0.82 a	0.30 d
9	21.83 b	716.8 b	2 654.2 c	699.3 c	3 326.1 b	997.2 c	0.65 b	2.59 b	0.45 b	0.55 b	0.79 a	0.31 d
10	18.08 c	572.3 d	2 263.3 d	619.5 d	2 755.9 c	985.2 c	0.63 b	2.23 b	0.32 c	0.44 c	0.76 a	0.42 c
11	12.91 d	496.4 e	1 759.4 e	611.5 d	2 349.2 d	992.3 c	0.61 b	2.24 b	0.34 c	0.43 c	0.63 b	0.43 c
12	7.70 e	332.2 f	1 596.3 e	513.2 e	1 855.3 e	987.2 c	0.59 b	1.53 c	0.23 d	0.33 d	0.55 c	0.58 b