Efficient regeneration system of Peucedanum praeruptorum Dunn based on adventitious bud induction

doi:10.16178/j.issn.0528-9017.20230078

Abstract

Abstract:

To establish an efficient regeneration system of Peucedanum praeruptorum Dunn, the effects of different concentrations of hormones on the induction and rooting of adventitious buds were studied with the stem tip tissues of wild Peucedanum praeruptorum Dunn as explants. The results showed that the best medium for adventitious bud induction was MS+2.5 mg·L^-1 6-BA, the induction rate reached 100%, and the multiplication rate of adventitious buds was 7.3 times. The best rooting medium was 1/2MS+0.2 mg·L^-1 NAA. The best transplanting method was to transplant the seedlings to the seedbed after 30 days-hydroponic training of the plants which are higher than 3 cm, with 3 to 5 roots over 2 cm. This system provides a new way for tissue culture and regeneration of Peucedanum praeruptorum Dunn and shortens the period of seedling breeding.

Key words: Peucedanum praeruptorum Dunn, tissue culture, adventitious buds, regeneration system

CLC Number:

S567.23

SHA Haodong, QIAN Yichun, XU Pan, PU Jinbao, GU Xueting, MAO Bizeng. Efficient regeneration system of Peucedanum praeruptorum Dunn based on adventitious bud induction[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(9): 2086-2092.

Figures/Tables 7

Table 1 Effect of phytohormone concentrations on adventitious buds induction of Peucedanum praeruptorum Dunn

BA浓度/ (mg·L^-1)	诱导率/ %	诱导倍数	不定芽长势
0.5	33	0.7	不定芽叶色浅绿、茎紫红
1.0	100	3.3	不定芽叶色翠绿,茎偏白
1.5	100	6.7	不定芽矮小、质地脆
2.0	100	4.3	不定芽较纤弱
2.5	100	7.3	不定芽叶色嫩绿、分株容易
3.0	100	13.0	不定芽矮小,无法分辨茎和叶,分株不容易

Fig.1 Effect of hormone concentrations on the induction of adventitious buds of Peucedanum praeruptorum Dunn

Fig.2 Effect of different hormones on the rooting of Peucedanum praeruptorum Dunn

Fig.3 Transplanting and domestication of tissue culture seedlings

Fig.4 Callus and structure of adventitious buds treated with different hormone concentrations

Fig.5 Newly sprouted adventitious buds(10×)

Fig.6 Submicroscopic structure of adventitious bud and callus cell

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