A preliminary report on the effect of different ratio of substrate on the growth of Tetrastigma hemsleyanum

doi:10.16178/j.issn.0528-9017.20230785

Abstract

Abstract:

Based on the production practice of the base, this study carried out the application experiments of different ratios of biochar and peat soil, observed the growth of roots, cane leaves and plants of Tetrastigma hemsleyanum, and evaluated the ecological improvement effect of biochar and peat soil, so as to provide technical support for the improvement of the quality of Tetrastigma hemsleyanum. Adding biochar to the substrate in the production of Tetrastigma hemsleyanum can reduce the substrate bulk density, increase the total porosity of the substrate, promote root development, increase substrate pH value, and promote the development of leaves. Moreover, due to the increase in the ratio of bacteria and fungi in the rhizosphere of Tetrastigma hemsleyanum by adding biochar, the substrate micro-environment tends to be bacterial, which is more favorable for its growth.

Key words: substrate ratio, Tetrastigma hemsleyanum, biochar, peat soil

CLC Number:

S359.9

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NI Jianping, WEN Junqing, LIU Yejun, JIANG Huaqin, DING Gengwei, MIAO Qiang, YANG Lujun. A preliminary report on the effect of different ratio of substrate on the growth of Tetrastigma hemsleyanum[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(5): 1232-1235.

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URL: http://www.zjnykx.cn/EN/10.16178/j.issn.0528-9017.20230785

http://www.zjnykx.cn/EN/Y2024/V65/I5/1232

Figures/Tables 7

Table 1 Experimental design

处理	基地常用基质/%	泥炭土/ %	无机肥/ %	生物质炭/%	有机肥/ %
CK	100	0	0	0	0
T1	0	60	0	20	20
T2	0	50	0	40	10
T3	0	80	20	0	0
T4	0	100	0	0	0
T5(脱毒)	0	80	20	0	0

Table 1 Experimental design

处理	基地常用基质/%	泥炭土/ %	无机肥/ %	生物质炭/%	有机肥/ %
CK	100	0	0	0	0
T1	0	60	0	20	20
T2	0	50	0	40	10
T3	0	80	20	0	0
T4	0	100	0	0	0
T5(脱毒)	0	80	20	0	0

Table 2 Physicochemical properties of substrates with different ratios

处理	容重/(g·m^-3)			总孔隙度/%			pH值
处理	0 d	25 d	45 d	0 d	25 d	45 d	0 d	25 d	45 d
CK	1.00	1.00	1.00	0.39	0.38	0.37	7.32	7.41	7.56
T1	0.36	0.36	0.36	0.52	0.54	0.59	8.26	8.07	7.83
T2	0.28	0.28	0.26	0.60	0.65	0.68	8.51	8.23	7.96
T3	0.55	0.56	0.54	0.45	0.46	0.48	7.92	7.42	7.33
T4	0.63	0.62	0.62	0.40	0.39	0.39	7.72	7.34	7.29
T5(脱毒)	0.51	0.53	0.52	0.45	0.45	0.44	8.03	7.18	7.22

Table 2 Physicochemical properties of substrates with different ratios

处理	容重/(g·m^-3)			总孔隙度/%			pH值
处理	0 d	25 d	45 d	0 d	25 d	45 d	0 d	25 d	45 d
CK	1.00	1.00	1.00	0.39	0.38	0.37	7.32	7.41	7.56
T1	0.36	0.36	0.36	0.52	0.54	0.59	8.26	8.07	7.83
T2	0.28	0.28	0.26	0.60	0.65	0.68	8.51	8.23	7.96
T3	0.55	0.56	0.54	0.45	0.46	0.48	7.92	7.42	7.33
T4	0.63	0.62	0.62	0.40	0.39	0.39	7.72	7.34	7.29
T5(脱毒)	0.51	0.53	0.52	0.45	0.45	0.44	8.03	7.18	7.22

Table 3 Soil conductivity of substrates with different ratios

处理	EC值/(mS·cm^-1)
处理	0 d	25 d	45 d
CK	0.65	0.46	0.33
T1	2.39	0.65	0.53
T2	2.59	0.77	0.61
T3	3.17	0.55	0.43
T4	1.01	0.47	0.36
T5(脱毒)	3.20	0.54	0.39

Table 3 Soil conductivity of substrates with different ratios

处理	EC值/(mS·cm^-1)
处理	0 d	25 d	45 d
CK	0.65	0.46	0.33
T1	2.39	0.65	0.53
T2	2.59	0.77	0.61
T3	3.17	0.55	0.43
T4	1.01	0.47	0.36
T5(脱毒)	3.20	0.54	0.39

Fig.1 Effects of substrates with different ratios on the number of rhizosphere microorganisms in Tetrastigma hemsleyanum

Table 4 Effects of substrates with different ratios on the growth of Tetrastigma hemsleyanum

处理	左小叶/cm		中小叶/cm		右小叶/cm
处理	叶长	叶宽	叶长	叶宽	叶长	叶宽
CK	3.98	1.53	5.36	1.93	4.12	1.49
T1	4.30	1.94	5.98	2.23	4.29	1.83
T2	4.16	2.46	5.54	2.77	3.76	2.41
T3	4.92	2.30	5.33	2.45	4.78	1.90
T4	3.93	2.16	5.29	2.17	3.55	1.85
T5(脱毒)	3.59	2.59	5.52	3.25	3.40	1.85

Table 4 Effects of substrates with different ratios on the growth of Tetrastigma hemsleyanum

处理	左小叶/cm		中小叶/cm		右小叶/cm
处理	叶长	叶宽	叶长	叶宽	叶长	叶宽
CK	3.98	1.53	5.36	1.93	4.12	1.49
T1	4.30	1.94	5.98	2.23	4.29	1.83
T2	4.16	2.46	5.54	2.77	3.76	2.41
T3	4.92	2.30	5.33	2.45	4.78	1.90
T4	3.93	2.16	5.29	2.17	3.55	1.85
T5(脱毒)	3.59	2.59	5.52	3.25	3.40	1.85

Table 5 Effects of different substrates on rhizomes of Tetrastigma hemsleyanum

处理	茎粗/cm	总根长/cm	总根鲜重/g
CK	0.211	10.328	3.214
T1	0.261	12.264	4.132
T2	0.272	12.187	4.085
T3	0.201	11.491	3.573
T4	0.270	10.965	3.516
T5(脱毒)	0.316	9.252	3.018

Table 5 Effects of different substrates on rhizomes of Tetrastigma hemsleyanum

处理	茎粗/cm	总根长/cm	总根鲜重/g
CK	0.211	10.328	3.214
T1	0.261	12.264	4.132
T2	0.272	12.187	4.085
T3	0.201	11.491	3.573
T4	0.270	10.965	3.516
T5(脱毒)	0.316	9.252	3.018

Table 6 Effects of plant ash on the growth of Tetrastigma hemsleyanum

处理	基地常用基质/%	有机肥(羊粪)/%	油茶饼/%	草木灰/%	茎长/cm	叶片数	总重/g	分枝数
T6	95	0	5	0	38.53	32.70	11.58	2.10
T7	92	8	0	0	41.09	21.00	6.31	1.80
T8	50	0	0	50	103.83	41.67	22.10	2.33
T9	65	0	0	35	128.73	37.00	24.50	2.02

Table 6 Effects of plant ash on the growth of Tetrastigma hemsleyanum

处理	基地常用基质/%	有机肥(羊粪)/%	油茶饼/%	草木灰/%	茎长/cm	叶片数	总重/g	分枝数
T6	95	0	5	0	38.53	32.70	11.58	2.10
T7	92	8	0	0	41.09	21.00	6.31	1.80
T8	50	0	0	50	103.83	41.67	22.10	2.33
T9	65	0	0	35	128.73	37.00	24.50	2.02

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