Abstract:

The effects of clove oil on blood biochemical indexes of juvenile Japanese pufferfish (Takifugu rubripes) were investigated to lay a foundation for analyzing the mechanism of anesthesia and the safety of its use. The anesthesia treatment of juvenile Japanese pufferfish was carried out with clove oil at concentrations of 8, 16 and 24 mg·L^-1, respectively, and blood biochemical indexes such as glucose (GLU), total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (T-BIL) and blood urea nitrogen (BUN) and serum lysozyme (LZM) activity in the fish were measured at 1/4, 4, 8, 24 h after anesthesia and 24 h after resuscitation.The results showed that clove oil anesthesia had a significant effect on blood biochemical indexes and lysozyme activity in juvenile Japanese pufferfish, and the effect was more significant at high anesthetic concentrations. After anesthetizing with clove oil and undergoing resuscitation for 24 h, most of the blood biochemical indexes and lysozyme activity of juvenile Japanese pufferfish were different from those before anesthesia. This experiment has concluded that the concentration range of clove oil suitable for the transportation and operation of juvenile Japanese pufferfish, which provides a theoretical basis for anesthesia of juvenile Japanese pufferfish with clove oil.

Key words: clove oil, Takifugu rubripes, blood biochemical indexes