Effects of different rhizobia inoculations on the growth of Medicago sativa L.cv.Zhongmu 10

doi:10.16178/j.issn.0528-9017.20240223

Abstract

Abstract:

Different rhizobia fungicide and fertilizer were used to inoculate Medicago sativa L.cv.Zhongmu 10, and the indicators of branching number, plant height, number of root nodules and biomass of Medicago sativa L. were measured at the early flowering stage to screen the fungicide and fertilizer that could improve the yield of Medicago sativa L.cv.Zhongmu 10. The results showed that, compared with the CK group, ACCC 17779 Chinese alfalfa rhizobia fungicide 5 mL (group A1) significantly increased the number of root nodules and underground biomass of Medicago sativa L., 10 mL (group A2) significantly increased the number of branches, plant height, the number of root nodules and underground biomass of Medicago sativa L.,15 mL (group A3) significantly increased the aboveground and underground biomass of Medicago sativa L.ACCC 17780 Chinese alfalfa rhizobia fungicide 5 mL (group B1) significantly increased the aboveground biomass of Medicago sativa L.,10 mL (group B2) significantly increased plant height, number of root nodules, aboveground and underground biomass of Medicago sativa L., and 15 mL (group B3) significantly increased underground biomass of Medicago sativa L. ACCC 03094 alfalfa rhizobial fungicide 5 mL (group C1) significantly increased the number of root nodules and underground biomass of Medicago sativa L, 10 mL (group C2) significantly increased the number of root nodules and underground biomass of Medicago sativa L., 15 mL (C3 group) significantly increased aboveground and underground biomass of Medicago sativa L. Huachuangjianong alfalfa rhizobia fungicide 5 mL (group D1) significantly increased the number of root nodules, 15 mL (group D3) significantly increased underground biomass of Medicago sativa L. Qiaonong rhizobia fertilizer 50 mL (group E1) and 100 mL (group E2) significantly increased the number of root nodules and root length, and 150 mL (E3 group) significantly increased the number of branches, root length, and aboveground and underground biomass of Medicago sativa L.. The above agents can be used as the main raw materials for the development of fungicide and fertilizer with the appropriate concentration for Medicago sativa L.cv.Zhongmu 10; Overall, Qiaonong rhizobia fertilizer has a lower cost and better cost-effectiveness compared with other agents.

Key words: rhizobia, Medicago sativa L.cv.Zhongmu 10, biomass

CLC Number:

S541.1

CHEN Peng, SUO Mingchun, DONG Wenli, WEN Jiefu, ZHANG Yarong, WANG Zhenyu, FAN Hailong, SUN Jie. Effects of different rhizobia inoculations on the growth of Medicago sativa L.cv.Zhongmu 10[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(6): 1412-1419.

Figures/Tables 9

References 24

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处理	菌剂名称	种子用量/g	用量梯度
A1	ACCC 17779中华苜蓿根瘤菌	11.2	5 mL菌剂+145 mL水
A2	ACCC 17779中华苜蓿根瘤菌	11.2	10 mL菌剂+140 mL水
A3	ACCC 17779中华苜蓿根瘤菌	11.2	15 mL菌剂+135 mL水
B1	ACCC 17780中华苜蓿根瘤菌	11.2	5 mL菌剂+145 mL水
B2	ACCC 17780中华苜蓿根瘤菌	11.2	10 mL菌剂+140 mL水
B3	ACCC 17780中华苜蓿根瘤菌	11.2	15 mL菌剂+135 mL水
C1	ACCC 03094苜蓿根瘤菌	11.2	5 mL菌剂+145 mL水
C2	ACCC 03094苜蓿根瘤菌	11.2	10 mL菌剂+140 mL水
C3	ACCC 03094苜蓿根瘤菌	11.2	15 mL菌剂+135 mL水
D1	华创佳农苜蓿根瘤菌剂	11.2	5 mL菌剂+145 mL水
D2	华创佳农苜蓿根瘤菌剂	11.2	10 mL菌剂+140 mL水
D3	华创佳农苜蓿根瘤菌剂	11.2	15 mL菌剂+135 mL水
E1	巧农根瘤菌肥	11.2	50 mL菌肥+100 mL水
E2	巧农根瘤菌肥	11.2	100 mL菌肥+50 mL水
E3	巧农根瘤菌肥	11.2	150 mL菌肥
CK	蒸馏水	11.2	150 mL水

处理	菌剂名称	种子用量/g	用量梯度
A1	ACCC 17779中华苜蓿根瘤菌	11.2	5 mL菌剂+145 mL水
A2	ACCC 17779中华苜蓿根瘤菌	11.2	10 mL菌剂+140 mL水
A3	ACCC 17779中华苜蓿根瘤菌	11.2	15 mL菌剂+135 mL水
B1	ACCC 17780中华苜蓿根瘤菌	11.2	5 mL菌剂+145 mL水
B2	ACCC 17780中华苜蓿根瘤菌	11.2	10 mL菌剂+140 mL水
B3	ACCC 17780中华苜蓿根瘤菌	11.2	15 mL菌剂+135 mL水
C1	ACCC 03094苜蓿根瘤菌	11.2	5 mL菌剂+145 mL水
C2	ACCC 03094苜蓿根瘤菌	11.2	10 mL菌剂+140 mL水
C3	ACCC 03094苜蓿根瘤菌	11.2	15 mL菌剂+135 mL水
D1	华创佳农苜蓿根瘤菌剂	11.2	5 mL菌剂+145 mL水
D2	华创佳农苜蓿根瘤菌剂	11.2	10 mL菌剂+140 mL水
D3	华创佳农苜蓿根瘤菌剂	11.2	15 mL菌剂+135 mL水
E1	巧农根瘤菌肥	11.2	50 mL菌肥+100 mL水
E2	巧农根瘤菌肥	11.2	100 mL菌肥+50 mL水
E3	巧农根瘤菌肥	11.2	150 mL菌肥
CK	蒸馏水	11.2	150 mL水

处理	有机质含量/(g·kg^-1)	pH值	全氮含量/%	水解性氮含量/(mg·kg^-1)
播种前	7.52±0.39 a	8.70±0.03 a	0.050±0.020 c	189.00±15.51 i
CK	5.56±0.64 bc	8.60±0.02 ab	0.117±0.010 b	230.00±10.61 gh
A1	6.06±0.53 b	8.50±0.05 b	0.059±0.010 bc	215.00±9.80 hi
A2	3.37±0.62 e	8.60±0.04 ab	0.075±0.030 bc	479.00±13.06 a
A3	4.13±0.45 de	8.60±0.03 ab	0.040 ±0.020 c	114.00±17.96 j
B1	5.35±0.40 bcd	8.60±0.05 ab	0.064±0.040 bc	278.00±19.60 ef
B2	5.32±0.73 bcd	8.50±0.07 b	0.051±0.030 c	317.00±8.98 d
B3	4.55±0.66 cde	8.70±0.02 a	0.121±0.020 ab	227.00±20.41 gh
C1	5.79±0.36 bc	8.70±0.05 a	0.178±0.030 a	305.00±23.68 de
C2	5.65±0.42 bc	8.60±0.07 ab	0.065±0.030 bc	305.00±9.80 de
C3	4.08±0.51 de	8.60±0.05 ab	0.179±0.040 a	244.00±8.98 fgh
D1	5.18±0.74 bcd	8.70±0.07 a	0.095±0.040 bc	189.00±19.60 i
D2	7.60±0.59 a	8.60±0.04 ab	0.062±0.040 bc	247.00±21.23 fgh
D3	7.97±0.66 a	8.70±0.06 a	0.031±0.010 c	253.00±13.06 fg
E1	8.73±0.48 a	8.60±0.03 ab	0.090±0.010 bc	339.00±13.88 cd
E2	7.88±0.68 a	8.60±0.06 ab	0.046±0.020 c	357.00±15.51 c
E3	8.83±0.78 a	8.60±0.05 ab	0.044±0.030 c	413.00±9.80 b

处理	有机质含量/(g·kg^-1)	pH值	全氮含量/%	水解性氮含量/(mg·kg^-1)
播种前	7.52±0.39 a	8.70±0.03 a	0.050±0.020 c	189.00±15.51 i
CK	5.56±0.64 bc	8.60±0.02 ab	0.117±0.010 b	230.00±10.61 gh
A1	6.06±0.53 b	8.50±0.05 b	0.059±0.010 bc	215.00±9.80 hi
A2	3.37±0.62 e	8.60±0.04 ab	0.075±0.030 bc	479.00±13.06 a
A3	4.13±0.45 de	8.60±0.03 ab	0.040 ±0.020 c	114.00±17.96 j
B1	5.35±0.40 bcd	8.60±0.05 ab	0.064±0.040 bc	278.00±19.60 ef
B2	5.32±0.73 bcd	8.50±0.07 b	0.051±0.030 c	317.00±8.98 d
B3	4.55±0.66 cde	8.70±0.02 a	0.121±0.020 ab	227.00±20.41 gh
C1	5.79±0.36 bc	8.70±0.05 a	0.178±0.030 a	305.00±23.68 de
C2	5.65±0.42 bc	8.60±0.07 ab	0.065±0.030 bc	305.00±9.80 de
C3	4.08±0.51 de	8.60±0.05 ab	0.179±0.040 a	244.00±8.98 fgh
D1	5.18±0.74 bcd	8.70±0.07 a	0.095±0.040 bc	189.00±19.60 i
D2	7.60±0.59 a	8.60±0.04 ab	0.062±0.040 bc	247.00±21.23 fgh
D3	7.97±0.66 a	8.70±0.06 a	0.031±0.010 c	253.00±13.06 fg
E1	8.73±0.48 a	8.60±0.03 ab	0.090±0.010 bc	339.00±13.88 cd
E2	7.88±0.68 a	8.60±0.06 ab	0.046±0.020 c	357.00±15.51 c
E3	8.83±0.78 a	8.60±0.05 ab	0.044±0.030 c	413.00±9.80 b

处理	全磷含量/ %	有效磷含量/ (mg·kg^-1)	全钾含量/ %	速效钾含量/ (mg·kg^-1)	水溶性盐总量/ (g·kg^-1)
播种前	0.040±0.011 a	6.00±0.57 a	3.03±0.18 a	61.00±3.27 cde	0.95±0.03 a
CK	0.039±0.009 a	3.80±0.41 cd	2.36±0.28 b	71.00±2.45 bc	0.70±0.04 b
A1	0.037±0.007 a	3.00±0.65 def	2.19±0.12 bc	65.00±4.90 cde	0.50±0.02 c
A2	0.037±0.007 a	2.40±0.49 ef	2.16±0.35 bc	65.00±1.63 cde	0.60±0.01 bc
A3	0.041±0.011 a	2.10±0.57 f	1.92±0.23 bc	56.00±0.82 e	0.70±0.06 b
B1	0.038±0.012 a	2.60±0.33 ef	2.19±0.16 bc	62.00±2.45 cde	0.60±0.07 bc
B2	0.038±0.010 a	2.60±0.41 ef	2.10±0.26 bc	65.00±3.27 cde	0.50±0.02 c
B3	0.039±0.013 a	2.40±0.24 ef	1.78±0.38 c	60.00±4.08 de	0.50±0.03 c
C1	0.041±0.010 a	3.30±0.73 de	2.23±0.27 bc	69.00±5.72 bcd	0.70±0.06 b
C2	0.041±0.009 a	3.30±0.65 de	2.23±0.12 bc	69.00±4.08 bcd	0.70±0.07 b
C3	0.040±0.004 a	5.30±0.41 ab	2.16±0.21 bc	69.00±4.90 bcd	0.60±0.02 bc
D1	0.039±0.006 a	3.30±0.16 de	2.20±0.29 bc	68.00±2.45 bcd	0.60±0.02 bc
D2	0.038±0.011 a	2.90±0.08 def	2.24±0.18 bc	65.00±6.53 cde	0.60±0.07 bc
D3	0.038±0.011 a	4.70±0.33 bc	2.24±0.14 bc	66.00±7.35 cd	0.50±0.07 c
E1	0.040±0.014 a	3.30±0.57 de	2.28±0.26 bc	81.00±1.63 a	0.70±0.04 b
E2	0.041±0.016 a	2.90±0.73 def	2.24±0.23 bc	77.00±5.72 ab	0.50±0.05 c
E3	0.031±0.009 a	5.10±0.41 ab	2.27±0.16 bc	70.00±3.27 bcd	0.50±0.01 c