Preliminary analysis of microecology in the soil of continuous cropping of Dioscorea opposita Thunb. in Yangjiang area, Guangdong Province

doi:10.16178/j.issn.0528-9017.20240732

Abstract

Abstract:

To explore the microbial composition and functional characteristics in the soil of continuous cropping of Dioscorea opposita Thunb. in Yangjiang area, soil samples of continuous cropping of Dioscorea opposita Thunb. were collected, their physicochemical properies were detected, and the composition and functional characteristics of the microbial community in the soil were analyzed by metagenomic sequencing method. The results showed that: the soil for continuous cropping of Dioscorea opposita Thunb. in Yangjiang area is acidic. The contents of total potassium and available potassium in the soil are relatively high, while the contents of available nitrogen and available phosphorus are relatively low. In terms of soil enzyme activity, acid phosphatase activity is relatively high while sucrase activity is the lowest. Among microorganisms, bacteria, archaea and fungi account for 88.90%, 9.84% and 1.07% respectively. Among the bacterial groups, the Pseudomonadota and Actinomycetes are the main ones. The archaea are mainly Nitrososphaerota and Thermoproteota. Among the fungal groups, Ascomycota, Basidiomycota and Mucormycota account for the major proportion. KEGG analysis indicates that amino acid metabolism, carbohydrate metabolism, cofactor and vitamin metabolism, and energy metabolism are significantly enriched in the microbial community. Resistance gene analysis indicates that the community contains resistance genes to rifamycin antibiotics, glycopeptide antibiotics, macrolide antibiotics, amino coumarin antibiotics, tetracycline antibiotics, monolactam antibiotics, etc. To sum up, when Dioscorea opposita Thunb. is grown continuously, the soil is acidic and the ratio of nitrogen, phosphorus and potassium is unbalanced. Soil microorganisms are mainly bacteria and archaea, and they contain a variety of antibiotic resistance genes. The results of this study provide a preliminary understanding of the composition and functional characteristics of soil microorganisms in continuous cropping of Dioscorea opposita Thunb. in Yangjiang area, and offer a basis for improving the obstacles of continuous cropping in the future.

Key words: Dioscorea opposita Thunb., rhizosphere soil, continuous cropping obstacle, soil microbiota

CLC Number:

S154.3

WU Fengwei, HUANG Lishan, XU Xiaofei. Preliminary analysis of microecology in the soil of continuous cropping of Dioscorea opposita Thunb. in Yangjiang area, Guangdong Province[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2502-2510.

Figures/Tables 4

Fig.1 Community composition and relative abundance of soil bacterial communities at the phylum and genus level

Fig.2 Relative abundance of soil archaea(A) and fungal(B) communities at the genus level

Fig.3 Analysis of the relative abundance composition of functional attributes of soil microbial communities

Fig.4 Compositions in relative abundance of antibiotic resistance ARO in the microbial communities

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