Effects of microplastics on the growth and cadmium accumulation of Brassica chinensis L.

doi:10.16178/j.issn.0528-9017.20250235

Abstract

Abstract:

The combined pollution of microplastics (MPs) and cadmium (Cd) in soils is becoming increasingly prevalent, posing potential threats to agricultural product safety. However, the interaction mechanisms and ecological risks between these pollutants remain unclear. In this study, a pot experiment was conducted with different concentrations of polypropylene (PP) MPs (0.25% and 1.00%) and Cd (0.649 mg·kg^-1) in single and combined treatments to investigate their effects on growth parameters, photosynthetic characteristics, and Cd accumulation in Brassica chinensis L.. The results demonstrated that PP alone exhibited concentration-dependent inhibitory effects on plant growth and photosynthesis, while Cd alone promoted plant growth. The combined exposure showed synergistic inhibitory effects and significantly enhanced Cd accumulation in both the aboveground and underground parts of Brassica chinensis L., with the effects intensifying at higher PP concentrations. This study revealed the toxic effects of individual and combined MPs-Cd pollution on Brassica chinensis L., providing important scienjpgic evidence for ensuring agricultural product safety and managing soil combined pollution.

Key words: soil microplastics, Brassica chinensis L., polypropylene, cadmium, plant accumulation

CLC Number:

S634.3

PAN Qianxuan, GUO Liang, GUO Jingjie, ZOU Lei, ZHENG Yining. Effects of microplastics on the growth and cadmium accumulation of Brassica chinensis L.[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(8): 1882-1890.

Figures/Tables 6

References 32

[1]	ROCHMAN C M, BROWNE M A, HALPERN B S, et al. Classify plastic waste as hazardous[J]. Nature, 2013, 494(7436): 169-171.
[2]	COLZI I, RENNA L, BIANCHI E, et al. Impact of microplastics on growth, photosynthesis and essential elements in Cucurbita pepo L[J]. Journal of Hazardous Materials, 2022, 423: 127238.
[3]	THOMPSON R C, OLSEN Y, MITCHELL R P, et al. Lost at sea: where is all the plastic?[J]. Science, 2004, 304(5672): 838.
[4]	RILLIG M C. Microplastic in terrestrial ecosystems and the soil?[J]. Environmental Science & Technology, 2012, 46(12): 6453-6454.
[5]	JING X Y, SU L C, WANG Y S, et al. How do microplastics affect physical properties of silt loam soil under wetting-drying cycles?[J]. Agronomy, 2023, 13(3): 844.
[6]	HANIF M N, AIJAZ N, AZAM K, et al. Impact of microplastics on soil (physical and chemical) properties, soil biological properties/soil biota, and response of plants to it: a review[J]. International Journal of Environmental Science and Technology, 2024, 21(16): 10277-10318.
[7]	LIU H Y, YUE L, ZHAO Y Y, et al. Changes in bacterial community structures in soil caused by migration and aging of microplastics[J]. Science of The Total Environment, 2022, 848: 157790.
[8]	JIANG L L, YE Y S, HAN Y L, et al. Microplastics dampen the self-renewal of hematopoietic stem cells by disrupting the gut microbiota-hypoxanthine-Wnt axis[J]. Cell Discovery, 2024, 10(1): 35.
[9]	刘勇, 马杰, 冯冰聪, 等. 微塑料迁移及其复合污染对土壤生态系统的影响研究进展[J]. 农业环境科学学报, 2025, 44(3): 537-553.
[10]	OSKARSSON A, WIDELL A, OLSSON I M, et al. Cadmium in food chain and health effects in sensitive population groups[J]. Biometals, 2004, 17(5): 531-534.
[11]	ZHAO F J, MA Y B, ZHU Y G, et al. Soil contamination in China: current status and mitigation strategies[J]. Environmental Science & Technology, 2015, 49(2): 750-759.
[12]	LI Q J, YAN J, LI Y L, et al. Microplastics alter cadmium accumulation in different soil-plant systems: Revealing the crucial roles of soil bacteria and metabolism[J]. Journal of Hazardous Materials, 2024, 474: 134768.
[13]	SARKER A, DEEPO D M, NANDI R, et al. A review of microplastics pollution in the soil and terrestrial ecosystems: a global and Bangladesh perspective[J]. Science of The Total Environment, 2020, 733: 139296.
[14]	LIN H, CUI G F, JIN Q, et al. Effects of microplastics on the uptake and accumulation of heavy metals in plants: a review[J]. Journal of Environmental Chemical Engineering, 2024, 12(1): 111812.
[15]	GUO J J, HUANG X P, XIANG L, et al. Source, migration and toxicology of microplastics in soil[J]. Environment International, 2020, 137: 105263.
[16]	GUO J J, LI F, XIAO H C, et al. Polyethylene and polypropylene microplastics reduce chemisorption of cadmium in paddy soil and increase its bioaccessibility and bioavailability[J]. Journal of Hazardous Materials, 2023, 449: 130994.
[17]	牛佳瑞, 邹勇军, 简敏菲, 等. 聚苯乙烯微塑料联合镉污染对土壤理化性质和生菜(Lactuca sativa)生理生态的影响[J]. 环境科学, 2024, 45(1): 470-479.
[18]	严馨, 赵建. 医用口罩原材料聚丙烯的生物降解研究进展[J]. 微生物前沿, 2021(2): 91-97.
[19]	ZHOU B Y, WANG J Q, ZHANG H B, et al. Microplastics in agricultural soils on the coastal plain of Hangzhou Bay, East China: multiple sources other than plastic mulching film[J]. Journal of Hazardous Materials, 2020, 388: 121814.
[20]	QI Y L, YANG X M, PELAEZ A M, et al. Macro- and micro-plastics in soil-plant system: effects of plastic mulch film residues on wheat (Triticum aestivum) growth[J]. Science of The Total Environment, 2018, 645: 1048-1056.
[21]	GUO J J, TAN X, FU H L, et al. Selection for Cd pollution-safe cultivars of Chinese kale (Brassica alboglabra L. H. bailey) and biochemical mechanisms of the cultivar-dependent Cd accumulation involving in Cd subcellular distribution[J]. Journal of Agricultural and Food Chemistry, 2018, 66(8): 1923-1934.
[22]	王学奎, 黄见良. 植物生理生化实验原理与技术[M]. 3版. 北京: 高等教育出版社, 2015.
[23]	TOMSETT A B, THURMAN D A. Molecular biology of metal tolerances of plants[J]. Plant, Cell & Environment, 1988, 11(5): 383-394.
[24]	杨金凤, 卜玉山, 郭小燕, 等. 土壤外源镉、铅污染对油菜生长的影响研究[J]. 陕西农业科学, 2005, 51(3): 25-28.
[25]	ZHOU Y H, XUE M, YANG Z Y, et al. High cadmium pollution risk on vegetable amaranth and a selection for pollution-safe cultivars to lower the risk[J]. Frontiers of Environmental Science & Engineering, 2013, 7(2): 219-230.
[26]	WANG W F, GE J, YU X Y, et al. Environmental fate and impacts of microplastics in soil ecosystems: progress and perspective[J]. Science of The Total Environment, 2020, 708: 134841.
[27]	LI J, SONG Y, CAI Y B. Focus topics on microplastics in soil: analytical methods, occurrence, transport, and ecological risks[J]. Environmental Pollution, 2020, 257: 113570.
[28]	SUN X D, YUAN X Z, JIA Y B, et al. Differentially charged nanoplastics demonstrate distinct accumulation in Arabidopsis thaliana[J]. Nature Nanotechnology, 2020, 15(9): 755-760.
[29]	FAJARDO C, MARTÍN C, COSTA G, et al. Assessing the role of polyethylene microplastics as a vector for organic pollutants in soil: ecotoxicological and molecular approaches[J]. Chemosphere, 2022, 288: 132460.
[30]	WU X, LIU Y, YIN S S, et al. Metabolomics revealing the response of rice (Oryza sativa L.) exposed to polystyrene microplastics[J]. Environmental Pollution, 2020, 266: 115159.
[31]	刘国锋, 何俊, 杨轶中, 等. 藻华聚集的生态效应: 对凤眼莲叶绿素和光合作用的影响[J]. 环境科学, 2015, 36(8): 2887-2894.
[32]	GAO M L, LIU Y, SONG Z G. Effects of polyethylene microplastic on the phytotoxicity of di-n-butyl phthalate in lettuce (Lactuca sativa L.var.ramosa Hort)[J]. Chemosphere, 2019, 237: 124482.

组别	地上部生物量(鲜重)/ (g·盆^-1)	BRS/%
CK+NPP	3.347±0.195 B b	0
CK+LPP	3.155±0.161 BC b	-5.736
CK+HPP	3.075±0.114 BC b	-8.127
Cd+NPP	3.848±0.117 A a	14.969
Cd+LPP	3.017±0.215 BC b	-9.860
Cd+HPP	2.684±0.232 C c	-19.809

组别	地上部生物量(鲜重)/ (g·盆^-1)	BRS/%
CK+NPP	3.347±0.195 B b	0
CK+LPP	3.155±0.161 BC b	-5.736
CK+HPP	3.075±0.114 BC b	-8.127
Cd+NPP	3.848±0.117 A a	14.969
Cd+LPP	3.017±0.215 BC b	-9.860
Cd+HPP	2.684±0.232 C c	-19.809

指标	Cd	PP	Cd × PP
地上部生物量	0.012 ns	25.800^***	9.979^**
地下部生物量	2.110 ns	38.950^***	11.060^**
株高	1.516 ns	37.679^***	4.396^*
根长	2.371 ns	28.473^***	2.627 ns
净光合速率	0.476 ns	46.192^***	28.710^***
叶绿素a	2.154 ns	75.573^***	7.104^**
叶绿素b	6.364^*	118.634^***	8.756^**
地上部Cd含量	2 025.190^***	168.723^***	44.556^***
地下部Cd含量	3 914.822^***	120.002^***	56.192^***

指标	Cd	PP	Cd × PP
地上部生物量	0.012 ns	25.800^***	9.979^**
地下部生物量	2.110 ns	38.950^***	11.060^**
株高	1.516 ns	37.679^***	4.396^*
根长	2.371 ns	28.473^***	2.627 ns
净光合速率	0.476 ns	46.192^***	28.710^***
叶绿素a	2.154 ns	75.573^***	7.104^**
叶绿素b	6.364^*	118.634^***	8.756^**
地上部Cd含量	2 025.190^***	168.723^***	44.556^***
地下部Cd含量	3 914.822^***	120.002^***	56.192^***

组别	BF	TF
CK+NPP	0.133	0.656
CK+LPP	0.193	0.664
CK+HPP	0.285	0.751
Cd+NPP	0.656	0.485
Cd+LPP	0.994	0.496
Cd+HPP	1.205	0.585