Abstract:

To investigate the effects of different fertilization dosage on carbon-nitrogen metabolism and alkaloid content in Zhusha tobacco, a field experiment was conducted in 2020 using Zhusha tobacco 3 as the test material. The results showed that the activities of nitrate reductase and glutamine synthetase in tobacco plants under the 15% fertilizer reduction treatment were lower than those under the 15% fertilizer increase treatment, indicating that nitrogen metabolism was less vigorous in the 15% fertilizer reduction treatment compared with the 15% fertilizer increase treatment. During the vigorous growth period, lower leaf maturation period, and middle leaf maturation period, the sucrose synthase activity in tobacco plants under the 15% fertilizer reduction treatment was significantly lower than that under the 15% fertilizer increase treatment, suggesting that the carbon metabolism products were less abundant and the carbon metabolism intensity was lower in the 15% fertilizer reduction treatment. Under the 15% fertilizer reduction and local conventional treatments, the nicotine content was lower than that under the 15% fertilizer increase treatment, but under the 15% fertilizer increase condition, the nornicotine content was the lowest.

Key words: different fertilization dosage, Zhusha tobacco, carbon-nitrogen metabolism, alkaloids