Effects of cadmium stress on osmotic regulation and balance of 3 clones of Taxodium hybrid Zhongshanshan

doi:10.16178/j.issn.0528-9017.20250168

Abstract

Abstract:

Utilizing Taxodium distichum, Zhongshanshan 118, and Zhongshanshan 406 as experimental materials, a hydroponic cultivation experiment was conducted to investigate the effects of heavy metal cadmium stress. The study assessed the osmotic adjustment system indicators such as leaf soluble sugars, proline, and soluble proteins content to compare the response characteristics of different Zhongshanshan clones under various cadmium concentrations. The objective was to select clones with cadmium tolerance and preliminarily explore the physiological mechanisms underlying their heavy metal cadmium resistance from the perspective of osmotic regulation. The findings indicate that under cadmium stress, the three Zhongshanshan clones displayed distinct response traits. Notably, Zhongshanshan 406 exhibited the most stable levels of leaf soluble sugars and soluble proteins content, and a pronounced increase in proline content, thereby demonstrating the most robust osmotic adjustment capacity in response to heavy metal cadmium stress.

Key words: cadmium stress, soluble sugar, soluble protein, proline, osmotic regulation, Taxodium hybrid Zhongshanshan

CLC Number:

S791.27

LI Min, LIU Fei, GAO Changjian, LIU Jinlin. Effects of cadmium stress on osmotic regulation and balance of 3 clones of Taxodium hybrid Zhongshanshan[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(9): 2194-2199.

Figures/Tables 3

References 17

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材料	镉浓度/ (μmol·L^-1)	可溶性糖含量/(mg·g^-1)
材料	镉浓度/ (μmol·L^-1)	第1周	第2周	第3周	第4周	第5周
落羽杉	0	13.467 c	13.582 c	13.825 c	16.459 c	18.116 c
	10	13.987 c	13.678 c	13.938 c	16.669 c	18.116 c
	50	18.863 b	18.408 b	16.962 b	22.309 b	21.155 b
	200	24.243 a	24.048 a	20.879 a	26.746 a	27.669 a
中山杉118	0	14.589 b	14.621 b	15.109 bc	16.913 b	17.319 b
	10	14.637 b	14.767 b	15.791 b	17.043 b	13.727 bc
	50	14.735 b	14.767 b	15.986 b	17.888 b	15.482 b
	200	26.015 a	23.772 a	20.781 a	24.146 a	20.570 a
中山杉406	0	14.312 b	14.345 b	15.027 bc	15.499 b	16.750 b
	10	14.377 b	14.491 b	15.401 b	15.726 b	16.848 b
	50	14.540 b	14.686 b	15.726 b	15.889 b	16.897 b
	200	22.780 a	22.878 a	19.757 a	23.983 a	19.270 a

材料	镉浓度/ (μmol·L^-1)	可溶性糖含量/(mg·g^-1)
材料	镉浓度/ (μmol·L^-1)	第1周	第2周	第3周	第4周	第5周
落羽杉	0	13.467 c	13.582 c	13.825 c	16.459 c	18.116 c
	10	13.987 c	13.678 c	13.938 c	16.669 c	18.116 c
	50	18.863 b	18.408 b	16.962 b	22.309 b	21.155 b
	200	24.243 a	24.048 a	20.879 a	26.746 a	27.669 a
中山杉118	0	14.589 b	14.621 b	15.109 bc	16.913 b	17.319 b
	10	14.637 b	14.767 b	15.791 b	17.043 b	13.727 bc
	50	14.735 b	14.767 b	15.986 b	17.888 b	15.482 b
	200	26.015 a	23.772 a	20.781 a	24.146 a	20.570 a
中山杉406	0	14.312 b	14.345 b	15.027 bc	15.499 b	16.750 b
	10	14.377 b	14.491 b	15.401 b	15.726 b	16.848 b
	50	14.540 b	14.686 b	15.726 b	15.889 b	16.897 b
	200	22.780 a	22.878 a	19.757 a	23.983 a	19.270 a

材料	镉浓度/ (μmol·L^-1)	可溶性蛋白含量/(mg·g^-1)
材料	镉浓度/ (μmol·L^-1)	第1周	第2周	第3周	第4周	第5周
落羽杉	0	1.314 c	1.387 c	1.417 c	1.962 c	2.527 c
	10	1.323 c	1.398 c	1.467 c	1.982 c	2.552 c
	50	2.402 ab	2.046 b	2.291 b	2.191 b	3.178 b
	200	2.619 a	3.234 a	3.331 a	3.746 a	3.849 a
中山杉118	0	1.286 b	1.306 b	1.362 b	1.615 b	1.601 b
	10	1.292 b	1.314 b	1.378 b	1.634 b	1.623 b
	50	1.303 b	1.395 b	1.395 b	1.654 b	1.645 b
	200	2.132 a	2.285 a	2.424 a	2.897 a	2.928 a
中山杉406	0	1.156 b	1.209 b	1.225 bc	1.473 b	1.439 b
	10	1.164 b	1.211 b	1.398 bc	1.573 b	1.531 b
	50	1.195 b	1.228 b	2.118 b	1.782 b	1.572 b
	200	1.985 a	2.013 a	2.107 a	2.216 a	2.600 a

材料	镉浓度/ (μmol·L^-1)	可溶性蛋白含量/(mg·g^-1)
材料	镉浓度/ (μmol·L^-1)	第1周	第2周	第3周	第4周	第5周
落羽杉	0	1.314 c	1.387 c	1.417 c	1.962 c	2.527 c
	10	1.323 c	1.398 c	1.467 c	1.982 c	2.552 c
	50	2.402 ab	2.046 b	2.291 b	2.191 b	3.178 b
	200	2.619 a	3.234 a	3.331 a	3.746 a	3.849 a
中山杉118	0	1.286 b	1.306 b	1.362 b	1.615 b	1.601 b
	10	1.292 b	1.314 b	1.378 b	1.634 b	1.623 b
	50	1.303 b	1.395 b	1.395 b	1.654 b	1.645 b
	200	2.132 a	2.285 a	2.424 a	2.897 a	2.928 a
中山杉406	0	1.156 b	1.209 b	1.225 bc	1.473 b	1.439 b
	10	1.164 b	1.211 b	1.398 bc	1.573 b	1.531 b
	50	1.195 b	1.228 b	2.118 b	1.782 b	1.572 b
	200	1.985 a	2.013 a	2.107 a	2.216 a	2.600 a

材料	镉浓度/ (μmol·L^-1)	脯氨酸含量/(mg·g^-1)
材料	镉浓度/ (μmol·L^-1)	第1周	第2周	第3周	第4周	第5周
落羽杉	0	57.872 c	58.587 c	61.179 cd	76.283 c	110.780 cd
	10	58.408 c	60.464 c	69.758 c	92.191 c	194.522 c
	50	80.501 b	106.848 b	130.532 b	137.949 b	199.438 b
	200	110.333 a	128.387 a	157.164 a	185.764 a	238.851 a
中山杉118	0	48.666 bc	66.898 b	92.906 c	122.577 d	165.029 d
	10	50.543 bc	69.222 b	111.942 c	135.268 bc	187.819 bc
	50	58.408 b	71.814 b	113.283 b	138.843 b	192.198 b
	200	84.684 a	249.218 a	215.703 a	216.597 a	225.445 a
中山杉406	0	46.343 b	62.966 b	92.101 b	103.184 bc	115.874 bc
	10	46.164 b	66.898 b	99.162 b	110.244 bc	120.879 bc
	50	47.683 b	71.367 b	105.597 b	117.126 b	126.242 b
	200	81.645 a	246.001 a	209.447 a	199.706 a	219.814 a