Purification technology of total flavonoids from Tetrastigma hemsleyanum

doi:10.16178/j.issn.0528-9017.20240320

Abstract

Abstract:

In order to optimize the process conditions for the separation and purification of total flavonoids from polyamide, the content of total flavonoids in Tetrastigma hemsleyanum was determined by ultraviolet spectrophotometry, and the flavonoids were adsorbed by polyamide resin, and the static and dynamic adsorption and desorption properties were studied from three aspects: static and dynamic adsorption and desorption performance after different treatments. The results showed that the optimal conditions for the adsorption of total flavonoids in the leaves of Tetrastigma hemsleyanum were 15 mg·mL^-1 and 1.75 g of polyamide in the loading solution, and the pH value was controlled at about 3.0 for static adsorption, and the highest adsorption rate was 76.7%. The polyamide resin was then desorbed with 60% ethanol with a desorption rate of up to 75.9%.

Key words: Tetrastigma hemsleyanum, flavonoids, polyamide resin, purification

CLC Number:

S567

FU Changyong, XU Haishun. Purification technology of total flavonoids from Tetrastigma hemsleyanum[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(8): 1759-1763.

Figures/Tables 9

Fig.1 Rutin standard curve and its linear equation

Fig.2 Effect of loading solution concentration on adsorption rate of polyamide

Fig.3 Effect of the weight of resin on the adsorption rate of polyamide

Fig.4 Effect of pH value on the adsorption rate of polyamide

Fig.5 Static adsorption curve

Fig.6 Effect of ethanol volume fraction on polyamide desorption

Fig.7 Static desorption curves

Fig.8 Leakage curve of polyamide resin

Fig.9 Elution curves of total flavonoids

References 12

[1]	刘伟民, 熊科辉, 刘洋, 等. 三叶青叶不同萃取物的抗氧化和抗炎活性评价[J]. 浙江林业科技, 2023, 43(5): 59-65.
[2]	MAO Z A, YANG L, LV Y S, et al. A glucuronogalactomannan isolated from Tetrastigma hemsleyanum Diels et Gilg: structure and immunomodulatory activity[J]. Carbohydrate Polymers, 2024, 333: 121922.
[3]	章健敏, 林国卫, 娄俊. 月均气温对三叶青林下容器栽培苗光合特性及其块根总黄酮含量的影响[J]. 浙江农业科学, 2024, 65(3): 555-561.
[4]	TAO K X, PENG H, BI X, et al. Effect of Tetrastigma hemsleyanum leaves and extract supplementation on liver metabolomics and the gut microbiota in alcohol-induced liver injury[J]. Food Bioscience, 2024, 58: 103617.
[5]	邓思珊, 刘洪旭, 马丽红, 等. 三叶青叶黄酮类化学成分的UPLC-MS/MS定性分析及HPLC含量测定[J]. 中国医药导报, 2018, 15(33): 80-84, 88.
[6]	鲁宪坤, 翟鑫源, 李方红, 等. 补骨脂总黄酮提取工艺的优化[J]. 中成药, 2022, 44(1): 193-197.
[7]	司建志, 王硕, 周小雷, 等. 聚酰胺纯化八角残渣黄酮的工艺研究[J]. 食品工业科技, 2015, 36(8): 245-249, 256.
[8]	杨阳, 张小杰, 高文运, 等. 聚酰胺纯化膜苞鸢尾中总黄酮的工艺研究[J]. 当代化工, 2020, 49(7): 1281-1284, 1289.
[9]	芦宇, 吕长鑫, 焦天慧, 等. 聚酰胺纯化红树莓籽黄酮及其结合胆酸盐能力评价[J]. 中国食品学报, 2019, 19(10): 172-178.
[10]	郑小卫, 李清林, 辛文秀, 等. 小春花总黄酮提取纯化工艺研究[J]. 中华中医药学刊, 2018, 36(4): 983-988.
[11]	撒玉良, 袁长胜, 陈文, 等. 聚酰胺树脂纯化玫瑰花总黄酮工艺研究[J]. 食品研究与开发, 2017, 38(21): 52-58.
[12]	陈俊其, 秦华珍, 尹优, 等. 山姜属中药黄酮类成分提取及分离富集方法研究进展[J]. 中医学报, 2019, 34(11): 2302-2307.