Study on tissue culture and rapid propagation technology of Nymphaea cv. Sunshine Princess

doi:10.16178/j.issn.0528-9017.20240465

Abstract

Abstract:

In this paper, the rhizoid of Nymphaea cv. Sunshine Princess was used as explants, and the culture conditions at different stages of Nymphaea cv. Sunshine Princess such as primary culture, proliferation, rooting and transplanting were studied comprehensively and systematically, and the tissue culture and rapid propagation technology system of waterlily was successfully established for the first time. The results showed that the solid medium was more suitable for tissue culture of Nymphaea cv. Sunshine Princess, and was beneficial to increase the proliferation rate and manual operation efficiency of waterlily. The effect of TDZ on the proliferation of buds was better than that of BA, but the two had a synergistic effect, and the proliferation ratio reached 3.44 and the growth state was good. The rooting effect of IBA was better than that of NAA at the same concentration, and the addition of activated carbon was also beneficial to the rooting induction of waterlily. Proper dosage of carbendazim could improve the survival rate of waterlily tissue culture seedlings. This study provides strong technical support for the aseptic preservation of waterlily germplasm resources and the rapid promotion of new varieties, and has important practical significance for accelerating the large-scale production of waterlily seedlings and promoting the industrial development of waterlily at home and abroad.

Key words: Nymphaea cv. Sunshine Princess, tissue culture, proliferation, rapid propagation

CLC Number:

S682.32

WANG Yan. Study on tissue culture and rapid propagation technology of Nymphaea cv. Sunshine Princess[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(10): 2283-2288.

Figures/Tables 6

References 30

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培养基类型	叶片颜色(老叶/新叶)	叶形	叶片数量/片	叶面积/cm²	叶片厚度/mm	叶柄长度/cm	增殖率
固液	绿色/红色	箭状卵形	7.13±0.71 b	11.71	0.31±0.03 a	16.08±5.9 a	1.00±0 b
固体	绿色/绿色	近圆形	10.87±3.04 a	3.39	0.21±0.05 b	8.25±2.15 b	1.65±0.46 a

培养基类型	叶片颜色(老叶/新叶)	叶形	叶片数量/片	叶面积/cm²	叶片厚度/mm	叶柄长度/cm	增殖率
固液	绿色/红色	箭状卵形	7.13±0.71 b	11.71	0.31±0.03 a	16.08±5.9 a	1.00±0 b
固体	绿色/绿色	近圆形	10.87±3.04 a	3.39	0.21±0.05 b	8.25±2.15 b	1.65±0.46 a

培养基编号	激素浓度(mg·L^-1)			起始材料数目	增殖不定芽数	增殖率	生长状态
培养基编号	TDZ	6-BA	NAA	起始材料数目	增殖不定芽数	增殖率	生长状态
1	0.5	0	0.1	18	31	1.72±0.39 d	正常
2	1.0	0	0.2	18	44	2.44±0.54 c	正常
3	2.0	0	0.2	18	51	2.83±0.51 c	正常
4	3.0	0	0.2	18	66	3.67±0.47 ab	异常
5	0	2.0	0.1	18	20	1.11±0.17 d	正常
6	0	4.0	0.2	18	31	1.72±0.49 d	正常
7	0	6.0	0.2	18	48	2.67±0.42 c	异常
8	1.0	1.0	0.2	18	52	2.88±0.34 c	正常
9	1.0	2.0	0.2	18	62	3.44±0.40 b	正常

培养基编号	激素浓度(mg·L^-1)			起始材料数目	增殖不定芽数	增殖率	生长状态
培养基编号	TDZ	6-BA	NAA	起始材料数目	增殖不定芽数	增殖率	生长状态
1	0.5	0	0.1	18	31	1.72±0.39 d	正常
2	1.0	0	0.2	18	44	2.44±0.54 c	正常
3	2.0	0	0.2	18	51	2.83±0.51 c	正常
4	3.0	0	0.2	18	66	3.67±0.47 ab	异常
5	0	2.0	0.1	18	20	1.11±0.17 d	正常
6	0	4.0	0.2	18	31	1.72±0.49 d	正常
7	0	6.0	0.2	18	48	2.67±0.42 c	异常
8	1.0	1.0	0.2	18	52	2.88±0.34 c	正常
9	1.0	2.0	0.2	18	62	3.44±0.40 b	正常

培养基编号	NAA/ (mg·L^-1)	IBA/ (mg·L^-1)	AC/ (g·L^-1)	接种芽数/ 个	生根数/ 条	生根苗数/ 株	生根率/ %	平均根数/ 条
A	0.10	0	0	30	69	22	73.3	2.76±0.33 d
B	0	0.10	0	30	118	29	96.7	3.93±0.43 c
C	0	0.10	0.1	30	142	30	100.0	4.73±0.65 b
D	0.05	0.05	0.1	30	256	30	100.0	8.53±1.08 a