Development and testing of a device for cleaning agar in tissue culture containers

doi:10.16178/j.issn.0528-9017.20230944

Abstract

Abstract:

A mechanical centrifugal technique was proposed to clean the agar in the tissue culture containers because of the difficulty of automatic cleaning due to the agar sticking to the wall of the cups. The effects of the rotation speed of the scraper, the rotation time of the scraper, the size of the scraper and the way of scraping in and out of the bottle on the cleaning effect of the containers by a one-way test. The orthogonal test was used to obtain the optimal combination of test conditions, and the results showed that the scraper rotation speed was 210 r·min^-1, the scraper rotation time was 2.5 s, the scraper in/out mode was positive-reverse rotation, the scraper radius was 28 mm, the effect of agar clearing of the container in the tissue culture reached more than 95%, which can meet the actual production requirements of the tissue culture container cleaning and filling. The test results provide technical support for the research and development of automatic cleaning and filling equipment for recycling-type tissue culture containers, and have the significance of engineering promotion.

Key words: tissue culture, agar, agar cleaning test, agar cleaning effect

CLC Number:

S226.5

ZHENG Hang, LIN Wenying, ZHANG Yi, XUE Xianglei, YE Yunxiang, CHEN Bing, YU Guohong. Development and testing of a device for cleaning agar in tissue culture containers[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(10): 2338-2343.

Figures/Tables 11

Fig.1 Schematic diagram of tissue culture container

Fig.2 Structure diagram of tissue culture container agar cleaning test device

Fig.3 Container agar cleaning rate at different rotation speeds of scraper

Fig.4 Container agar cleaning rate at different rotation time

Fig.5 Container agar cleaning rate at different scraper sizes

Fig.6 Container agar cleaning rate at different scraper in/out mode

Table 1 Orthogonal test factors and levels

水平	因素
水平	A刮片转速/(r·min^-1)	B旋转时间/s	C进出形式
1	150	2.5	正转-正转
2	180	3.0	正转-停止
3	210	3.5	正转-反转

Table 2 Experimental results and analysis of variance

序号	因素				清脂率/ %
序号	A	B	C	空列	清脂率/ %
1	1	1	1	1	94.34
2	1	2	2	2	89.77
3	1	3	3	3	95.34
4	2	1	2	3	91.16
5	2	2	3	1	97.80
6	2	3	1	2	98.74
7	3	1	3	2	97.61
8	3	2	1	3	99.61
9	3	3	2	1	92.56
k₁	93.15	94.37	97.56	94.90
k₂	95.90	95.73	91.16	95.37
k₃	96.59	95.55	96.92	95.37
R	3.44	1.36	6.40	0.47

Fig.7 Tissue culture container agar clearing test

Table 3 Validation test results of container agar clearing rate

序号	清脂前容器质量/g	清脂后容器质量/g	空瓶质量/g	清脂率/ %
1	219.26	186.84	186.29	98.33
2	209.38	174.51	173.97	98.48
3	209.93	173.46	173.05	98.89

Fig.8 Comparative results of before and after agar clearing test of tissue culture container

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