Effects of oxalic acid on rhizosphere bacterial diversity of flax in cadmium-contaminated soil

doi:10.16178/j.issn.0528-9017.20240275

Abstract

Abstract:

In order to study the effects of oxalic acid on rhizosphere bacteria of flax in cadmium-contaminated soil, a pot experiment was conducted. The rhizosphere soil of flax was taken at the physiological maturity stage, and the effects of different concentrations of oxalic acid on rhizosphere bacterial diversity and community composition of flax in cadmium-contaminated soil were analyzed by Illumina-MiSeq high-throughput sequencing technology. The results showed that the treatment with high concentration of oxalic acid could increase the OTU number of rhizosphere bacteria in the cadmium-contaminated soil. ACE, Chao1, Simpson and Shannon index values were the highest under the treatment with 2.5 mmol·kg^-1 oxalic acid, among which Simpson and Shannon index values were significantly higher than the control. The results of PCoA analysis showed that the community structure of bacterial OTU in rhizosphere soil of flax treated with high concentration of oxalic acid was the most different from that in control. In terms of bacterial composition, the dominant bacteria phylum and dominant bacteria genus were Patescibacteria and TM7a, respectively, with an average proportion of 40.60% and 26.06%, respectively. In summary, the treatment with 2.5 mmol·kg^-1of oxalic acid can increase the diversity and abundance of rhizosphere bacteria in cadmium-contaminated soil, and has the greatest effect on bacterial composition. This study can be helpful to provide a theoretical reference for the subsequent research on the effects of oxalic acid and other exogenous organic acids on cadmium absorption in flax.

Key words: oxalic acid, flax, rhizosphere, bacterial diversity

CLC Number:

S532

LIU Tingting, ZOU Lina, LUO Xiahong, LI Wenlue, CHEN Changli, ZHU Guanlin, AN Xia. Effects of oxalic acid on rhizosphere bacterial diversity of flax in cadmium-contaminated soil[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(7): 1764-1769.

Figures/Tables 6

References 21

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样品名称	有效序列数目/条	总长度/ bp	最大长度/ bp	最小长度/ bp	OTU数量/ 个
CK-1	111 812	51 027 445	474	201	1 872
CK-2	103 777	47 039 247	474	202	2 051
CK-3	101 592	46 218 601	474	202	1 833
OA1-1	109 563	49 807 858	474	201	2 112
OA1-2	111 101	50 507 042	474	202	1 669
OA1-3	98 785	44 790 113	474	201	1 681
OA2-1	99 809	45 142 020	474	202	1 984
OA2-2	102 732	46 493 559	474	202	2 036
OA2-3	109 951	49 747 733	474	202	2 308
OA3-1	103 206	46 739 607	474	202	2 358
OA3-2	103 438	46 926 580	474	206	2 265
OA3-3	99 622	45 013 130	474	202	2 013

样品名称	有效序列数目/条	总长度/ bp	最大长度/ bp	最小长度/ bp	OTU数量/ 个
CK-1	111 812	51 027 445	474	201	1 872
CK-2	103 777	47 039 247	474	202	2 051
CK-3	101 592	46 218 601	474	202	1 833
OA1-1	109 563	49 807 858	474	201	2 112
OA1-2	111 101	50 507 042	474	202	1 669
OA1-3	98 785	44 790 113	474	201	1 681
OA2-1	99 809	45 142 020	474	202	1 984
OA2-2	102 732	46 493 559	474	202	2 036
OA2-3	109 951	49 747 733	474	202	2 308
OA3-1	103 206	46 739 607	474	202	2 358
OA3-2	103 438	46 926 580	474	206	2 265
OA3-3	99 622	45 013 130	474	202	2 013

样品名称	覆盖度/%	ACE指数	Chao1指数	Shannon指数	Simpson指数
CK	99.29	2 173.90±90.23 ab	2 055.48±100.32 ab	5.99±0.26 b	0.92±0.01 b
OA1	99.28	2 101.83±213.01 b	1 980.94±225.35 b	6.18±0.89 b	0.94±0.03 ab
OA2	99.20	2 383.41±155.70 ab	2 254.19±150.29 ab	6.69±0.37 ab	0.94±0.02 ab
OA3	99.28	2 465.14±183.28 a	2 334.68±165.32 a	7.32±0.49 a	0.96±0.02 a

样品名称	覆盖度/%	ACE指数	Chao1指数	Shannon指数	Simpson指数
CK	99.29	2 173.90±90.23 ab	2 055.48±100.32 ab	5.99±0.26 b	0.92±0.01 b
OA1	99.28	2 101.83±213.01 b	1 980.94±225.35 b	6.18±0.89 b	0.94±0.03 ab
OA2	99.20	2 383.41±155.70 ab	2 254.19±150.29 ab	6.69±0.37 ab	0.94±0.02 ab
OA3	99.28	2 465.14±183.28 a	2 334.68±165.32 a	7.32±0.49 a	0.96±0.02 a