Isolation and identification of pepper anthracnose pathogen in Lyuchun County，Yunnan Province of China

doi:10.16178/j.issn.0528-9017.20240360

Abstract

Abstract:

Anthracnose is a common disease on pepper leaves，which can cause plant leaves to wither and fall-off in severe cases，affecting pepper yield and causing certain economic losses. Colletotrichum fungi are important pathogens that cause the withering of pepper branches and leaves. Lyuchun County is the main pepper production area in Yunnan Province，and due to the local humid and hot climate，pepper anthracnose occurs frequently. This study isolated and purified a pathogenic fungus strain H4 from diseased tissues，and determined its pathogenicity. Based on morphological characteristics and the combined construction of internal transcribed spacer （ITS） sequence， Actin gene，and GADPH gene，a phylogenetic tree analysis was conducted，and it was determined that the pathogen causing anthracnose in pepper leaves was Colletotrichum siamense. To our knowledge，this was the first discovery of anthracnose on pepper leaves in Yunnan caused by C. siamense. And the relevant results provide important theoretical basis for the prevention and control of pepper diseases.

Key words: anthracnose, pepper（Piper nigrum L.）, pathogen, identification, Colletotrichum siamense

CLC Number:

S435.621.2⁺2

HE Guoyou, ZHANG Yijie, SHI Chunyue, LU Yao, FU Chengxiu, WANG Zhenchong, YAN Qin, KONG Chuisi. Isolation and identification of pepper anthracnose pathogen in Lyuchun County，Yunnan Province of China[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(2): 444-448.

Figures/Tables 5

Table 1 Primers used for pathogen identification

基因	引物	序列（5′→3′）	参考文献
ITS	ITS1	TCCGTAGGTGAACCTGCGG	［9］
ITS	ITS2	TCCTCCGCTTATTGATATGC	［9］
Actin	ACT-512F	ATGTGCAAGGCCGGTTTCGC	［9］
Actin	ACT-783R	TACGAGTCCTTCTGGCCCAT	［9］
GAPDH	GDF1	GCCGTCAACGACCCCTTCATTGA	［9］
GAPDH	GDR1	GGGTGGAGTCGTACTTGAGCATGT	［9］

Fig.1 Symptoms of Piper nigrum L. leaf anthracnose

Fig.2 Morphological appearances of colonies on PDA plates

Fig.3 Morphological characteristics of the pathogen of C. siamense

Fig.4 Phylogenetic tree of Colletotrichum based on combined ITS，Actin and GAPDH genes

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