Callus induction and identification of glyphosate resistance in indica rice Minghui 86

doi:10.16178/j.issn.0528-9017.20250820

Abstract

Abstract:

To establish the regeneration system of indica rice Minghui 86，the mature seeds of Minghui 86 were used as materials，and five different mass concentrations of hormone culture media were designed for callus induction and proliferation. The callus induction rate of indica rice Minghui 86 in combination C ［1.0 mg·L^-16-benzyladenine （6-BA）+2.5 mg·L^-12，4-dichlorophenoxyacetic acid （2，4-D）］ was the highest，reaching 92.3%，indicating that combination C was the best for callus induction of Minghui 86. On this basis，the callus of indica rice Minghui 86 was selected and six mass concentrations of glyphosate（0，100，200，300，400 and 500 mg·L^-1）were set up to screen the glyphosate resistance of callus. When the mass concentration of glyphosate was in the range of 300-400 mg·L^-1，the screening effect was best. Three more concentrations（300，350 and 400 mg·L^-1）were set，and the critical concentration was 300 mg·L^-1. The optimal hormone combination for callus induction and proliferation of indica rice Minghui 86 was found，which provided technical support for the development of glyphosate-resistant rice.

Key words: indica rice Minghui 86, callus, regeneration system, glyphosate

CLC Number:

S511

FU Zhuangzhuang, GONG Yuhang, LI Feifei. Callus induction and identification of glyphosate resistance in indica rice Minghui 86[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(5): 1221-1225.

Figures/Tables 4

Table 1 Five hormone combinations for callus induction and proliferation

激素组合	6-苄氨基嘌呤（6-BA）	2，4-二氯苯氧乙酸（2，4-D）
A	0	2.0
B	0	2.5
C	1.0	2.5
D	0	3.0
E	0	4.0

Fig.1 Callus induction of indica rice Minghui 86 under different hormone treatments

Fig.2 Callus proliferation of indica rice Minghui 86 under different hormone treatments

Fig.3 Browning rate of indica rice Minghui 86 callus treated with different mass concentrations of glyphosate

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