Abstract:

Using the stem segments of kiwifruit as materials, callus induction was induced on MS medium, and the response surface methodology was used to optimize the induction conditions of kiwifruit callus. On the basis of single factor analysis, a three factor three-level response surface methodology was designed to optimize the experiment, with 6-BA mass concentration, 2, 4-D mass concentration, and 0.1% mercuric chloride treatment time as independent variables, and the induction rate of callus tissue in the stem segment of kiwifruit as the evaluation index. The results showed that the optimal conditions for inducing callus tissue in kiwifruit were 6-BA concentration of 0.4 mg·L^-1, 2, 4-D concentration of 2.0 mg·L^-1, and 0.1% mercuric chloride treatment time of 4.0 minutes. Under these induction conditions, the induction rate of callus tissue in kiwifruit stem segments was 85.71%, which had a relatively small relative error with the predicted value. The experimental value was in good agreement with the predicted value. After optimizing the induction conditions, the induction rate of callus tissue in kiwifruit stem segments was effectively improved, providing a basis for further research on kiwifruit tissue culture.

Key words: Guichang kiwifruit, callus, stem segments, tissue culture, response surface methodology