浙江农业科学

浙江农业科学 ›› 2024, Vol. 65 ›› Issue (2): 449-453.DOI: 10.16178/j.issn.0528-9017.20230964

• 蚕桑与特种养殖 • 上一篇    下一篇

丁香油对红鳍东方鲀幼鱼血液生化指标的影响

王舒慧(), 赵睿虎, 曹新宇, 李欣阳, 姜志强()   

  1. 大连海洋大学 农业农村部北方海水增养殖重点实验室,辽宁 大连 116023
  • 收稿日期:2023-09-26 出版日期:2024-02-11 发布日期:2024-02-28
  • 通讯作者: 姜志强(1960—),男,山东乳山人,教授,研究方向为水产养殖,E-mail:zhqjiang@dlou.edu.cn。
  • 作者简介:姜志强(1960—),男,山东乳山人,教授,研究方向为水产养殖,E-mail:zhqjiang@dlou.edu.cn。
    王舒慧(1999—),女,辽宁海城人,硕士,研究方向为水产养殖,E-mail:1186017227@qq.com。
  • 基金资助:
    2023中央财政对辽宁渔业补助项目

Effects of clove oil on blood biochemical indexes in juvenile Japanese pufferfish (Takifugu rubripes)

Shuhui WANG(), Ruihu ZHAO, Xinyu CAO, Xinyang LI, Zhiqiang JIANG()   

  1. Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023,Liaoning
  • Received:2023-09-26 Online:2024-02-11 Published:2024-02-28
  • Contact: Zhiqiang JIANG

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摘要：

通过研究丁香油对红鳍东方鲀(Takifugu rubripes)幼鱼血液生化指标的影响,为分析其麻醉机理及使用安全性奠定基础。分别用8、16、24 mg·L-1浓度的丁香油溶液,对红鳍东方鲀幼鱼进行麻醉处理,在麻醉后1/4、4、8、24 h以及复苏后24 h,对鱼体血糖(GLU)、血清中总蛋白(TP)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(T-BIL)、尿素氮(BUN)等血液生化指标和血清溶菌酶(LZM)活性进行测定。结果显示,丁香油麻醉对红鳍东方鲀幼鱼的多项血液生化指标以及血清溶菌酶活性均产生了显著影响,且该影响在高麻醉浓度下更显著。经丁香油麻醉及复苏24 h后,大部分的幼鱼血液生化指标与血清溶菌酶活性与未麻醉前不同。该试验得出了适宜红鳍东方鲀幼鱼运输和操作的丁香油浓度范围,为丁香油麻醉红鳍东方鲀幼鱼提供理论依据。

关键词: 丁香油, 红鳍东方鲀(Takifugu rubripes), 血液生化指标

Abstract:

The effects of clove oil on blood biochemical indexes of juvenile Japanese pufferfish (Takifugu rubripes) were investigated to lay a foundation for analyzing the mechanism of anesthesia and the safety of its use. The anesthesia treatment of juvenile Japanese pufferfish was carried out with clove oil at concentrations of 8, 16 and 24 mg·L-1, respectively, and blood biochemical indexes such as glucose (GLU), total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (T-BIL) and blood urea nitrogen (BUN) and serum lysozyme (LZM) activity in the fish were measured at 1/4, 4, 8, 24 h after anesthesia and 24 h after resuscitation.The results showed that clove oil anesthesia had a significant effect on blood biochemical indexes and lysozyme activity in juvenile Japanese pufferfish, and the effect was more significant at high anesthetic concentrations. After anesthetizing with clove oil and undergoing resuscitation for 24 h, most of the blood biochemical indexes and lysozyme activity of juvenile Japanese pufferfish were different from those before anesthesia. This experiment has concluded that the concentration range of clove oil suitable for the transportation and operation of juvenile Japanese pufferfish, which provides a theoretical basis for anesthesia of juvenile Japanese pufferfish with clove oil.

Key words: clove oil, Takifugu rubripes, blood biochemical indexes

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