浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (6): 1420-1424.DOI: 10.16178/j.issn.0528-9017.20231228

• 观赏园艺 • 上一篇    下一篇

台湾佛甲草的优质高效组培快繁技术

詹菁(), 沈林骏   

  1. 杭州萧山技师学院,浙江 杭州 311202
  • 收稿日期:2024-12-25 出版日期:2025-06-11 发布日期:2025-06-23
  • 作者简介:詹菁(1984—),女,高级讲师,硕士,研究方向为园林植物与观赏园艺,E-mail:365712408@qq.com。
  • 基金资助:
    2024年浙江省中华职业教育科研项目—乡村振兴背景下“产学研培创”五位一体农业实训基地建设路径研究

High quality and efficient tissue culture and rapid propagation technology of Sedum formosanum N.E.Br.

ZHAN Jing(), SHEN Linjun   

  1. Hangzhou Xiaoshan Technician College, Hangzhou 311202, Zhejiang
  • Received:2024-12-25 Online:2025-06-11 Published:2025-06-23

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摘要：

本研究以台湾佛甲草茎段为试验材料,通过对外植体进行预处理及无菌化处理获取无菌苗,随后将外植体转移到不同的增殖培养基及生根培养基上进行梯度试验,对比分析、筛选出台湾佛甲草最适合的组培配方。结果表明,在外植体取材前7 d,隔天使用800~1 000倍的50%多菌灵溶液对母本苗进行喷施,可有效减少内生菌,有效提高外植体的无菌化成活率。诱导培养基中加入0.2%的植物组培抗菌剂,结合75%乙醇30 s、0.1% HgCl2 8 min的灭菌处理,外植体无菌化成活率最高达76%;以基本培养基(MS)+6-苄基腺嘌呤(6-BA)1.5 mg·L-1+α-萘乙酸(NAA)0.1 mg·L-1+蔗糖30 g·L-1+琼脂8 g·L-1作增殖培养基时增殖效果较好,增殖系数达21.4;台湾佛甲草在1/2 MS培养基上生根效果良好,生根率达100%。本研究建立了台湾佛甲草组培快繁体系,为其工厂化生产、丰富我国地被植物品种资源奠定了一定基础。

关键词: 台湾佛甲草, 杀菌剂, 地被植物

Abstract:

This study utilized stem segments of Sedum formosanum N.E.Br. as experimental materials. Sterile seedlings were obtained through pretreatment and sterilization of explants. Subsequently, the explants were transferred to different proliferation and rooting media for gradient experiments, followed by comparative analysis to screen the optimal tissue culture formulation. The results showed that spraying the mother plants with 800-1 000 times diluted 50% carbendazim solution every other day in one week prior to explant collection effectively reduced endophytic bacteria and improved the survival rate of sterile explants. The highest sterile survival rate of explants (76%) was achieved by adding 0.2% plant tissue culture antimicrobial agent to the induction medium, combined with sterilization using alcohol for 30 s and 0.1% HgCl2 for 8 min. The proliferation medium MS + 1.5 mg·L-1 6-BA + 0.1 mg·L-1 NAA + 30 g·L-1 sucrose + 8 g·L-1 agar demonstrated superior proliferation effects, with a proliferation coefficient of 21.4. Sedum formosanum N.E.Br. exhibited excellent rooting performance on 1/2 MS medium, achieving a 100% rooting rate. This study established a tissue culture rapid propagation system for Sedum formosanum N.E.Br., laying a foundation for its industrialized production and enriching China's groundcover plant species resources.

Key words: Sedum formosanum N.E.Br., fungicides, groundcover

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