月季HSP20基因家族鉴定及功能分析

doi:10.16178/j.issn.0528-9017.20240700

摘要/Abstract

摘要：

热休克蛋白20(HSP20)在植物生长、发育和对非生物胁迫响应中发挥关键作用。尽管在许多植物中,HSP20基因都与热胁迫有关,但月季中HSP20(RcHSP20)基因家族却鲜有研究。为了研究 RcHSP20对热胁迫的响应机制,基于全基因组鉴定分析了月季中HSP20基因家族的所有成员,共鉴定出 31个RcHSP20基因,并对其理化性质进行了分析。系统进化分析表明,RcHSP20可分为4个亚类。重复事件分析表明,串联重复和片段化复制事件在促进RcHSP20基因家族的扩张中发挥了关键作用。共线性分析表明,月季与玫瑰的同源性最高,有21个同源基因,其次是拟南芥和水稻,分别有5个和2个同源基因。在CⅠ亚家族中,RcHSP20-7、RcHSP20-8、RcHSP20-1、RcHSP20-14、RcHSP20-2、RcHSP20-3以及RcHSP20-5的表达模式呈现出高度的相似性:在受到0.5 h的热胁迫后表达量开始上升,当热胁迫时间延长至2 h,基因表达量大幅增加,直至达到峰值。然而,热胁迫6~12 h这些基因的表达量开始逐步降低。结果表明,这些RcHSP20基因在参与热胁迫响应中可能扮演着至关重要的角色。

关键词: 月季, HSP20, 生物信息学, 基因家族

Abstract:

Heat shock protein 20 (HSP20) plays a critical role in plant growth, development, and response to abiotic stress. Although HSP20 genes have been associated with heat stress in numerous types of plants, there is little research on HSP20 (RcHSP20) gene family in Rosa chinensis. In order to investigate the response mechanism of RcHSP20 to heat stress, all members of HSP20 gene family in R. chinensis were identified and analyzed based on whole genome identification. A total of 31 RcHSP20 genes were identified and their physicochemical properties were analyzed. System evolution analysis shows that RcHSP20 can be divided into 4 subtypes. The duplicated event analysis demonstrated that tandem duplication and segment duplication events played crucial roles in promoting the expansion of RcHSP20 gene family. Collinearity analysis showed that R. chinensis shared the highest homology in 21 HSP20 gene pairs with Rosa rugosa, followed by 5 and 2 homologous gene pairs with Arabidopsis thaliana and Oryza sativa, respectively. The expression pattern of RcHSP20-7, RcHSP20-8, RcHSP20-1, RcHSP20-14, RcHSP20-2, RcHSP20-3 and RcHSP20-5 in the CⅠ subfamily showed a high degree of similarity. After being subjected to 0.5 hours of heat stress, the expression level began to increase. When the heat stress time was prolonged to 2 hours, the gene expression level increased until reaching its peak. However, the expression levels of these genes gradually decreased after 6-12 hours of heat stress. The results indicate that these RcHSP20 genes may play a crucial role in participating in heat stress response.

Key words: Rosa chinensis, HSP20, bioinformatics, gene family

中图分类号:

S685.12

侯哲, 陈靓颖, 姜正之, 沈萍, 武金翠, 黄长兵. 月季HSP20基因家族鉴定及功能分析[J]. 浙江农业科学, 2025, 66(12): 3026-3035.

HOU Zhe, CHEN Liangying, JIANG Zhengzhi, SHEN Ping, WU Jincui, HUANG Changbing. Identification and functional analysis of HSP20 gene family in Rosa chinensis[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(12): 3026-3035.

图/表 9

参考文献 18

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基因号	基因名称	染色体	基因组位置	氨基酸数量	分子量/ku	等电点	分类
RcHSP20-1	XP_024177208.1	chr1	58 942 964~58 943 810	156	17.48	5.97	CⅠ
RcHSP20-2	XP_024158424.1	chr5	69 056 274~69 057 015	154	17.83	5.83	CⅠ
RcHSP20-3	XP_024198633.1	chr5	69 069 909~69 070 669	154	17.87	6.20	CⅠ
RcHSP20-4	XP_024162486.1	chr6	68 368 642~68 369 402	157	17.50	5.95	CⅡ
RcHSP20-5	XP_024159125.1	chr5	69 100 788~69 101 533	154	17.87	6.20	CⅠ
RcHSP20-6	XP_024177207.1	chr1	58 943 643~58 945 009	162	18.35	8.04	CⅠ
RcHSP20-7	XP_024191667.1	chr1	58 881 195~58 881 969	158	17.95	8.80	CⅠ
RcHSP20-8	XP_024195982.1	chr4	65 247 181~65 247 933	158	18.01	6.77	CⅠ
RcHSP20-9	XP_024162485.1	chr6	68 370 621~68 371 407	195	21.82	9.27	CⅡ
RcHSP20-10	XP_024166911.1	chr1	67 429 577~67 430 674	225	24.80	7.77	P
RcHSP20-11	XP_024185453.1	chr2	86 643 306~86 643 990	147	16.82	6.84	CⅠ
RcHSP20-12	XP_024181394.1	chr2	53 006 324~53 007 776	227	25.51	6.61	MⅡ
RcHSP20-13	XP_024162484.1	chr6	68 371 476~68 372 689	207	22.99	5.66	CⅡ
RcHSP20-14	XP_024158547.1	chr5	17 089 977~17 091 008	151	17.39	5.57	CⅠ
RcHSP20-15	XP_024195338.1	chr4	56 053 354~56 054 498	233	26.28	8.90	P
RcHSP20-16	XP_024186557.1	chr3	37 802 222~37 803 178	145	16.36	8.01	Po
RcHSP20-17	XP_024181213.1	chr2	64 678 952~64 679 826	161	17.79	6.74	CⅢ
RcHSP20-18	XP_024172893.1	chr7	20 732 219~20 733 489	223	24.95	5.48	MⅠ
RcHSP20-19	XP_024198077.1	chr5	2 226 808~2 227 700	139	16.27	4.98	CⅤ
RcHSP20-20	XP_024158220.1	chr5	73 158 751~73 159 609	142	15.86	4.53	CⅣ
RcHSP20-21	XP_024175290.1	chr1	62 242 944~62 243 892	204	23.40	6.01	MⅠ
RcHSP20-22	XP_024174092.1	chr7	304 037~305 038	221	25.00	8.57	CⅦ
RcHSP20-23	XP_024191057.1	chr3	47 646 284~47 647 154	166	18.86	9.17	Po
RcHSP20-24	XP_024166392.1	chr1	21 429 962~21 431 466	394	42.68	9.42	CⅦ
RcHSP20-25	XP_024158947.1	chr5	47 359 990~47 361 559	334	37.32	7.58	Po
RcHSP20-26	XP_024178515.1	chr2	51 759 480~51 760 285	147	16.82	8.66	Po
RcHSP20-27	XP_040375573.1	chr5	31 531 529~31 533 387	164	19.19	5.46	CⅥ
RcHSP20-28	XP_024163883.1	chr6	11 882 555~11 883 007	150	16.86	5.26	MⅠ
RcHSP20-29	XP_024172565.1	chr7	2 485 836~2 486 933	268	30.26	5.74	CⅦ
RcHSP20-30	XP_024182745.1	chr2	94 06 366~9 413 678	570	42.84	4.89	CⅦ
RcHSP20-31	XP_024190984.1	chr3	47 524 330~47 525 133	151	17.27	9.60	Po

基因号	基因名称	染色体	基因组位置	氨基酸数量	分子量/ku	等电点	分类
RcHSP20-1	XP_024177208.1	chr1	58 942 964~58 943 810	156	17.48	5.97	CⅠ
RcHSP20-2	XP_024158424.1	chr5	69 056 274~69 057 015	154	17.83	5.83	CⅠ
RcHSP20-3	XP_024198633.1	chr5	69 069 909~69 070 669	154	17.87	6.20	CⅠ
RcHSP20-4	XP_024162486.1	chr6	68 368 642~68 369 402	157	17.50	5.95	CⅡ
RcHSP20-5	XP_024159125.1	chr5	69 100 788~69 101 533	154	17.87	6.20	CⅠ
RcHSP20-6	XP_024177207.1	chr1	58 943 643~58 945 009	162	18.35	8.04	CⅠ
RcHSP20-7	XP_024191667.1	chr1	58 881 195~58 881 969	158	17.95	8.80	CⅠ
RcHSP20-8	XP_024195982.1	chr4	65 247 181~65 247 933	158	18.01	6.77	CⅠ
RcHSP20-9	XP_024162485.1	chr6	68 370 621~68 371 407	195	21.82	9.27	CⅡ
RcHSP20-10	XP_024166911.1	chr1	67 429 577~67 430 674	225	24.80	7.77	P
RcHSP20-11	XP_024185453.1	chr2	86 643 306~86 643 990	147	16.82	6.84	CⅠ
RcHSP20-12	XP_024181394.1	chr2	53 006 324~53 007 776	227	25.51	6.61	MⅡ
RcHSP20-13	XP_024162484.1	chr6	68 371 476~68 372 689	207	22.99	5.66	CⅡ
RcHSP20-14	XP_024158547.1	chr5	17 089 977~17 091 008	151	17.39	5.57	CⅠ
RcHSP20-15	XP_024195338.1	chr4	56 053 354~56 054 498	233	26.28	8.90	P
RcHSP20-16	XP_024186557.1	chr3	37 802 222~37 803 178	145	16.36	8.01	Po
RcHSP20-17	XP_024181213.1	chr2	64 678 952~64 679 826	161	17.79	6.74	CⅢ
RcHSP20-18	XP_024172893.1	chr7	20 732 219~20 733 489	223	24.95	5.48	MⅠ
RcHSP20-19	XP_024198077.1	chr5	2 226 808~2 227 700	139	16.27	4.98	CⅤ
RcHSP20-20	XP_024158220.1	chr5	73 158 751~73 159 609	142	15.86	4.53	CⅣ
RcHSP20-21	XP_024175290.1	chr1	62 242 944~62 243 892	204	23.40	6.01	MⅠ
RcHSP20-22	XP_024174092.1	chr7	304 037~305 038	221	25.00	8.57	CⅦ
RcHSP20-23	XP_024191057.1	chr3	47 646 284~47 647 154	166	18.86	9.17	Po
RcHSP20-24	XP_024166392.1	chr1	21 429 962~21 431 466	394	42.68	9.42	CⅦ
RcHSP20-25	XP_024158947.1	chr5	47 359 990~47 361 559	334	37.32	7.58	Po
RcHSP20-26	XP_024178515.1	chr2	51 759 480~51 760 285	147	16.82	8.66	Po
RcHSP20-27	XP_040375573.1	chr5	31 531 529~31 533 387	164	19.19	5.46	CⅥ
RcHSP20-28	XP_024163883.1	chr6	11 882 555~11 883 007	150	16.86	5.26	MⅠ
RcHSP20-29	XP_024172565.1	chr7	2 485 836~2 486 933	268	30.26	5.74	CⅦ
RcHSP20-30	XP_024182745.1	chr2	94 06 366~9 413 678	570	42.84	4.89	CⅦ
RcHSP20-31	XP_024190984.1	chr3	47 524 330~47 525 133	151	17.27	9.60	Po

基因复制	类型	Ka值	Ks值	Ka/Ks值	进化模式	分化时间(百万年)
RcHSP20-2/RcHSP20-3	串联重复	0.013 9	0.052 9	0.265 9	纯化选择	62.71
RcHSP20-4/RcHSP20-9	串联重复	0.066 0	0.183 0	0.359 0	纯化选择	19.39
RcHSP20-9/RcHSP20-13	串联重复	0.314 0	1.074 0	0.292 0	纯化选择	38.42
RcHSP20-30/RcHSP20-24	片段化复制	0.937 0	2.519 0	0.372 0	纯化选择	45.76

基因复制	类型	Ka值	Ks值	Ka/Ks值	进化模式	分化时间(百万年)
RcHSP20-2/RcHSP20-3	串联重复	0.013 9	0.052 9	0.265 9	纯化选择	62.71
RcHSP20-4/RcHSP20-9	串联重复	0.066 0	0.183 0	0.359 0	纯化选择	19.39
RcHSP20-9/RcHSP20-13	串联重复	0.314 0	1.074 0	0.292 0	纯化选择	38.42
RcHSP20-30/RcHSP20-24	片段化复制	0.937 0	2.519 0	0.372 0	纯化选择	45.76