血红丛赤壳属交配群Ⅵ全基因组候选效应分子的预测和分析

doi:10.16178/j.issn.0528-9017.20210137

摘要/Abstract

摘要：

血红丛赤壳属交配群Ⅵ(Nectria haematococca mating population Ⅵ)是茄镰孢豌豆专化型(Fusarium solani f. sp. pisi)的有性态,由其引起的豌豆镰孢根腐病是豌豆上最重要的病害之一。对该菌候选效应分子进行预测,并对其特征进行明确。通过全基因组测序获得Nectria haematococca mpⅥ LQ1菌株全基因组信息,利用Signal P、TMHMM、Protcomp、big-PI Predictor 和Target P等生物信息学软件和预测程序对其中9 912条蛋白序列进行候选效应分子预测,再通过对上述蛋白半胱氨酸含量、信号肽长度和冗余性分析,获得229个符合条件的候选效应分子,其中大部分为功能未知的假定蛋白。利用生物信息学方法预测出血红丛赤壳属交配群Ⅵ LQ1的候选效应分子,为进一步研究血红丛赤壳属交配群Ⅵ的致病机制奠定了基础,并为其他病原菌效应分子的预测与分析提供了参考。

关键词: 血红丛赤壳属交配群Ⅵ, 效应分子, 基因组, 生物信息学

中图分类号:

S432.4⁺4

戚家明, 张东旭, 王少丽, 王阳, 肖建中. 血红丛赤壳属交配群Ⅵ全基因组候选效应分子的预测和分析[J]. 浙江农业科学, 2021, 62(1): 106-110.

图/表 5

参考文献 16

[1]	ETEBU E, OSBORN A M. Pea footrot disease depends on the combination of pathogenicity genes in Nectria haematococca[J]. Asian Journal of Agricultural Sciences, 2011, 3(3):156-161.
[2]	宋刚, 徐玉明. 豌豆品种抗根腐病鉴定初报[J]. 杂粮作物, 2001, 21(4):40-41.
[3]	JONES J D G, DANGL J L. The plant immune system[J]. Nature, 2006, 444(7117):323-329. DOI URL PMID
[4]	STERGIOPOULOS I, DE WIT P J G M. Fungal effector proteins[J]. Annual Review of Phytopathology, 2009, 47:233-263. DOI URL PMID
[5]	BOLTON M D, VAN ESSE H P, VOSSEN J H, et al. The novel Cladosporium fulvum lysin motif effector Ecp6 is a virulence factor with orthologues in other fungal species[J]. Molecular Microbiology, 2008, 69(1):119-136. DOI URL PMID
[6]	VANETTEN H, FUNNELL-BAERG D, WASMANN C, et al. Location of pathogenicity genes on dispensable chromosomes in Nectria haematococca MPVI[J]. Antonie Van Leeuwenhoek, 1994, 65(3):263-267. DOI URL PMID
[7]	HAN Y N, LIU X G, BENNY U, et al. Genes determining pathogenicity to pea are clustered on a supernumerary chromosome in the fungal plant pathogen Nectria haematococca[J]. The Plant Journal, 2001, 25(3):305-314. URL PMID
[8]	TEMPORINI E D, VANETTEN H D. Distribution of the pea pathogenicity (PEP) genes in the fungus Nectria haematococca mating population VI[J]. Current Genetics, 2002, 41(2):107-114. DOI URL PMID
[9]	VANETTEN H D, MATTHEWS P S, TEGTMEIER K J, et al. The association of pisatin tolerance and demethylation with virulence on pea in Nectria haematococca[J]. Physiological Plant Pathology, 1980, 16(2):257-268. DOI URL
[10]	VANETTEN H D. Identification of additional habitats of Nectria haematococca mating population Ⅵ[J]. Phytopathology, 1978, 68(11):1552. DOI URL
[11]	REP M, KISTLER H C. The genomic organization of plant pathogenicity in Fusarium species[J]. Current Opinion in Plant Biology, 2010, 13(4):420-426. URL PMID
[12]	YOSHIDA K, SAITOH H, FUJISAWA S, et al. Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae[J]. The Plant Cell, 2009, 21(5):1573-1591. DOI URL PMID
[13]	韩长志. 全基因组预测希金斯炭疽菌的候选效应分子[J]. 生物技术, 2015, 25(6):546-551.
[14]	闫丽斌, 肖淑芹, 薛春生. 玉米大斑病菌全基因组候选效应分子的预测和分析[J]. 沈阳农业大学学报, 2017, 48(1):15-20.
[15]	SULZENBACHER G, MACKENZIE L F, WILSON K S, et al. The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 Å resolution[J]. Biochemistry, 1999, 38(15):4826-4833. DOI URL PMID
[16]	MA Z C, ZHU L, SONG T Q, et al. A paralogous decoy protects Phytophthora sojae apoplastic effector PsXEG1 from a host inhibitor[J]. Science, 2017, 355(6326):710-714. URL PMID

基因ID	同源性/%	E-value	描述
A0508	88.5	1.30×10^-124	polysaccharide lyase family 3
A0880	89.2	8.50×10^-55	Mg²⁺ transporter protein,cora-like/zinc transport protein zntb
A1026	58.8	2.70×10^-18	chymotrypsin-elastase inhibitor ixodidin
A2234	98.9	8.00×10^-106	plastocyanin-like domain-containing protein
A2700	50.7	1.40×10^-36	basic form of pathogenesis-protein 1
A3201	95.9	1.60×10^-135	polysaccharide lyase family 3
A3403	49.2	1.30×10^-50	AAC-rich mrna clone aac11 protein
A3468	96.1	1.10×10^-131	polysaccharide lyase family 3
A4018	99.1	2.40×10^-136	polysaccharide lyase family 3
A4804	93.5	7.80×10^-107	bacterial-type extracellular deoxyribonuclease
A5722	96.4	1.50×10^-139	glycoside hydrolase family 12
A5836	98.4	4.50×10^-101	phospholipase A2
A7641	95.3	1.20×10^-139	polysaccharide lyase family 3
A8305	66.7	3.80×10^-26	hydrophobin 3 precursor
A8369	97.8	1.80×10^-123	carbohydrate esterase family 5
A9623	82.8	1.50×10^-140	polysaccharide lyase family 1

基因ID	同源性/%	E-value	描述
A0508	88.5	1.30×10^-124	polysaccharide lyase family 3
A0880	89.2	8.50×10^-55	Mg²⁺ transporter protein,cora-like/zinc transport protein zntb
A1026	58.8	2.70×10^-18	chymotrypsin-elastase inhibitor ixodidin
A2234	98.9	8.00×10^-106	plastocyanin-like domain-containing protein
A2700	50.7	1.40×10^-36	basic form of pathogenesis-protein 1
A3201	95.9	1.60×10^-135	polysaccharide lyase family 3
A3403	49.2	1.30×10^-50	AAC-rich mrna clone aac11 protein
A3468	96.1	1.10×10^-131	polysaccharide lyase family 3
A4018	99.1	2.40×10^-136	polysaccharide lyase family 3
A4804	93.5	7.80×10^-107	bacterial-type extracellular deoxyribonuclease
A5722	96.4	1.50×10^-139	glycoside hydrolase family 12
A5836	98.4	4.50×10^-101	phospholipase A2
A7641	95.3	1.20×10^-139	polysaccharide lyase family 3
A8305	66.7	3.80×10^-26	hydrophobin 3 precursor
A8369	97.8	1.80×10^-123	carbohydrate esterase family 5
A9623	82.8	1.50×10^-140	polysaccharide lyase family 1