浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (10): 2352-2358.DOI: 10.16178/j.issn.0528-9017.20250462

• 药用植物 • 上一篇    下一篇

掌叶覆盆子根活性成分对未成熟果实品质的指示作用分析

汪传宝1(), 江治良2, 胡淳莉3, 俞国良4, 占建勇2, 姜玲2, 郑平汉2, 王志安1,5, 孙健1,5,*()   

  1. 1.浙江省中药研究所有限公司,浙江 杭州 310023
    2.淳安县农业农村局,浙江 淳安 311700
    3.淳安县食品药品检验检测中心,浙江 淳安 311700
    4.临安区於潜镇中药材办公室,浙江 杭州 311311
    5.道地药材品质保障与资源持续利用全国重点实验室, 北京 100700
  • 收稿日期:2025-06-27 出版日期:2025-10-11 发布日期:2025-10-21
  • 通讯作者: 孙健,男,山东莱州人,高级工程师,博士,主要从事中药材抗性育种和生态栽培,E-mail:21116025@zju.edu.cn。
  • 作者简介:汪传宝,男,安徽黄山人,硕士,主要从事中药资源品质评价,E-mail:1712740643@qq.com。
  • 基金资助:
    名贵中药资源可持续利用能力建设项目(2060302-2202-09);浙江省农业(中药材新品种选育)新品种选育重大科技专项(2021C02074);浙江省科技计划(省属科研院所扶持专项)

Analysis of the indicative role of active ingredients in the roots of Rubus chingii Hu on the quality of immature fruits

WANG Chuanbao1(), JIANG Zhiliang2, HU Chunli3, YU Guoliang4, ZHAN Jianyong2, JIANG Ling2, ZHENG Pinghan2, WANG Zhian1,5, SUN Jian1,5,*()   

  1. 1. Zhejiang Research Institute of Traditional Chinese Medicine Co., Ltd., Hangzhou 310023, Zhejiang
    2. Agriculture and Rural Affairs Bureau of Chun'an County, Chun'an 311700, Zhejiang
    3. Food and Drug Testing Center of Chun'an County, Chun'an 311700, Zhejiang
    4. Chinese Herbal Medicine Office of Yuqian Town, Lin'an District, Hangzhou 311311, Zhejiang
    5. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing 100700
  • Received:2025-06-27 Online:2025-10-11 Published:2025-10-21

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摘要： 本研究利用高效液相色谱法(HPLC),分别对掌叶覆盆子根和未成熟果实中活性成分进行测定,将根中鞣花酸和没食子酸含量分别与未成熟果实中鞣花酸和山柰酚-3-O-芸香糖苷含量进行相关分析。结果显示,本研究中掌叶覆盆子根部鞣花酸含量为0.88%~2.02%,没食子酸含量为0.52%~1.10%;未成熟果实中鞣花酸含量为0.07%~0.32%,山柰酚-3-O-芸香糖苷含量为0.03%~0.16%。根中鞣花酸和没食子酸总含量与未成熟果实中鞣花酸的含量呈现出线性相关,决定系数R2为0.740 9。当根中鞣花酸和没食子酸总含量高于2.60%时,其未成熟果实中鞣花酸含量预测值高于《中华人民共和国药典》指标要求。根中鞣花酸和没食子酸含量与未成熟果实中山柰酚-3-O-芸香糖苷含量未呈现线性相关。本研究表明,可以通过在休眠期测定根部鞣花酸和没食子酸含量对果实中鞣花酸含量高的育种材料进行预筛选。

关键词: 掌叶覆盆子, 鞣花酸, 没食子酸, 山柰酚-3-O-芸香糖苷

Abstract:

In this study, high-performance liquid chromatography (HPLC) was applied to determine the active ingredients in the roots and immature fruits of Rubus chingii Hu. Correlation analyses were conducted between the contents of ellagic acid and gallic acid in roots and the contents of ellagic acid and kaempferol-3-O-rutinoside in immature fruits respectively. The results showed that the content of ellagic acid in the roots of Rubus chingii Hu was 0.88%-2.02%, and the content of gallic acid was 0.52%-1.10%. The content of ellagic acid in immature fruits was 0.07%-0.32%, and the content of kaempferol-3-O-rutinoside was 0.03%-0.16%. The total content of ellagic acid and gallic acid in roots showed a linear correlation with the content of ellagic acid in immature fruits, with the determination coefficient R2 being 0.740 9. When the total content of ellagic acid and gallic acid in roots was higher than 2.60%, the predicted value of ellagic acid content in immature fruits was higher than the index requirement of Pharmacopoeia of the People's Republic of China. No linear correlation was found between the contents of ellagic acid and gallic acid in roots and the content of kaempferol-3-O-rutinoside in immature fruits. This study indicates that determining the contents of ellagic acid and gallic acid in roots during the dormancy period can be used for pre-screening breeding materials with high levels of ellagic acid in fruits.

Key words: Rubus chingii Hu, ellagic acid, gallic acid, kaempferol-3-O-rutinoside

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