浙江农业科学

浙江农业科学 ›› 2024, Vol. 65 ›› Issue (11): 2693-2702.DOI: 10.16178/j.issn.0528-9017.20240181

• 食品技术 • 上一篇    下一篇

番木瓜TCP转录因子鉴定及在果实成熟中的表达

周汪洋1,2(), 吴飞燕2, 赵苗羽2, 黄蕾2, 陈雄进3, 周艳2,4,5,*(), 刘锴栋2,4,5,*()   

  1. 1.广东海洋大学 滨海农业学院, 广东 湛江 524088
    2.岭南师范学院 生命科学与技术学院, 广东 湛江 524048
    3.湛江市农业科学研究院, 广东 湛江 524094
    4.广东省热带特色资源植物科技创新中心,广东 湛江 524048
    5.湛江市热带特色资源植物技术开发重点实验室,广东 湛江 524048
  • 收稿日期:2024-03-07 出版日期:2024-11-11 发布日期:2024-11-15
  • 通讯作者: 周艳 (1991—),女,四川南充人,副教授,博士,研究方向为园艺植物逆境生理和分子生物学,E-mail:286138826@qq.com;刘锴栋 (1982—),男,广东湛江人,教授,博士,研究方向为果树分子生物学,E-mail:liukaidong2001@126.com。
  • 作者简介:周汪洋 (2000—),女,湖南常德人,硕士,研究方向为果树生物技术,E-mail:183261938@qq.com。
  • 基金资助:
    国家自然科学基金面上项目(32372414);广东省自然科学基金项目(2022A1515010719);广东省自然科学基金项目(2023A1515012212);广东省科技专项资金项目(2021A05192);广东省科技专项资金项目(2021A05222);岭南师范学院雷阳学者岗位计划项目(2022);广东省大学生科技创新培育项目(pdjh2023b0328)

Identification of TCP transcription factors in Carica papaya and their expression during fruit ripening

ZHOU Wangyang1,2(), WU Feiyan2, ZHAO Miaoyu2, HUANG Lei2, CHEN Xiongjin3, ZHOU Yan2,4,5,*(), LIU Kaidong2,4,5,*()   

  1. 1. College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, Guangdong
    2. Life Science & Technology School, Lingnan Normal University, Zhanjiang 524048,Guangdong
    3. Zhanjiang Academy of Agricultural Sciences, Zhanjiang 524094, Guangdong
    4. Guangdong Technology Innovation Center of Tropical Characteristic Plant Resource Development, Zhanjiang 524048, Guangdong
    5. Zhanjiang Key Laboratory of Tropical Characteristic Plant Technology Development, Zhanjiang 524048, Guangdong
  • Received:2024-03-07 Online:2024-11-11 Published:2024-11-15

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摘要：

TCP蛋白是植物特有的转录因子, 在植物生长发育、信号转导以及逆境响应等方面发挥着重要作用。该研究利用生物信息学方法对番木瓜TCP基因家族成员进行系统鉴定, 并对其进化关系、保守基序、保守结构域、基因结构、蛋白三级结构和其在果实成熟中表达模式进行了相关分析。结果表明, 番木瓜TCP基因家族中含有22个成员;TCP基因家族成员可分为2大类:Class Ⅰ和Class Ⅱ, 其中Class Ⅱ可细分为CYC/TB1和CIN两个亚类;同一亚组内, TCP蛋白具有相似的保守基序和保守结构域, 不同亚组之间具有较大差异, 表明不同类型TCP蛋白可能具有独特的生理功能;22个家族成员均有bHLH(basic-helix-loop-helix)保守区, 且其基因结构由1~7个外显子和1~6个内含子构成;Class Ⅱ亚族基因表达量随着果实的成熟显著下调, 推测Class Ⅱ亚族成员能够抑制果实成熟。这些结果有助于今后揭示番木瓜TCP基因家族成员的功能。

关键词: 番木瓜, TCP转录因子, 生物信息学分析, 表达分析

Abstract:

TCP protein is the plant specific transcription factors, which plays an important role in the aspects of plant growth and development, signal transduction and biochemical stimulation response. In this research, we systematically screened the family members of TCP gene of Carica papaya and analyzed their phylogenetic relationship, conserved motifs, conserved domains, gene structure, protein tertiary structure and expression pattern in fruit maturation by using the bioinformatics methods. The results showed that 22 TCP genes existed in the Carica papaya TCP gene family. TCP gene family could be divided into two classes: Class Ⅰ and Class Ⅱ, and Class Ⅱ could be further classified into two subclasses (CYC/TB1 and CIN). Within the same subgroup, TCP proteins had similar conserved motifs and conserved domains, and there were large differences between different subgroups, indicating that different types of TCP proteins might have unique physiological functions. The 22 family members all had bHLH (basic-helix-loop-helix) conserved region and their genetic structure consisted of 1-7 exon and 1-6 introns; Gene expression in Class Ⅱ subgroup decreased significantly with fruit ripening, so it was assumed that Class Ⅱ subgroup had no effect on fruit ripening or inhibited fruit ripening. The above results would be helpful to reveal the function of TCP gene family members in Carica papaya.

Key words: Carica papaya, TCP transcription factor, bioinformatics analysis, expression pattern

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