超高温堆肥在有机固体废弃物抗生素抗性基因等生物污染消减中的研究进展

doi:10.16178/j.issn.0528-9017.20230913

摘要/Abstract

摘要：

抗生素抗性基因(antibiotic resistance genes,ARGs)污染已经成为一个严重的环境问题。高温堆肥是一项高效、绿色的有机固废无害化处理方式,促进物质循环利用,减少抗生素及其ARGs的排放,但是传统高温堆肥具有发酵周期较长、无害化不彻底、消减ARGs和移动遗传元素效果较差等局限性,超高温堆肥(ultra-high temperature composting, UHTC)是近年来开发的一种有机固体废弃物资源化新技术,其具有发酵温度高、周期短、无害化效果强、资源化效率高和运行成本低等特点。然而,不同UHTC系统在去除有机固体废弃物中的ARGs效果仍存在显著差异,值得深入讨论及总结。因此,本研究对UHTC技术对有机固体废弃物中ARGs的降解效果及机制进行了深入探讨,旨在进一步加强为有机固体废物中ARGs控制提供理论支持。

关键词: 抗生素抗性基因, 有机固体废弃物, 超高温堆肥

Abstract:

The pollution of antibiotic resistance genes (ARGs) has become a serious environmental problem. High temperature composting is an efficient and green harmless treatment method for organic solid waste, which promotes the recycling of substances and reduces the emission of antibiotics and ARGs. However, traditional high temperature composting has some limitations such as long fermentation cycle, incomplete harmless treatment, and poor effect on reducing ARGs and mobile genetic elements. Ultra-high temperature composting (UHTC) is a new technology for recycling organic solid waste developed in recent years. It has the characteristics of high fermentation temperature, short cycle, strong harmless effect, high recycling efficiency and low operating cost. However, different UHC composting systems still have significant differences in the removal of ARGs from organic solid waste, which is worthy of further discussion and summary. Therefore, the degradation effect and mechanism of ultra-high temperature composting technology on ARGs in organic solid waste were discussed in this paper, aiming to further strengthen the theoretical support for the control of ARGs in organic solid waste.

Key words: antibiotic resistance gene, organic solid waste, ultra-high temperature compost

中图分类号:

S141.3
X53

王经邦, 王亚菲, 姚燕来, 朱凤香, 王卫平, 洪春来, 朱为静, 洪磊东. 超高温堆肥在有机固体废弃物抗生素抗性基因等生物污染消减中的研究进展[J]. 浙江农业科学, 2024, 65(12): 3061-3067.

WANG Jingbang, WANG Yafei, YAO Yanlai, ZHU Fengxiang, WANG Weiping, HONG Chunlai, ZHU Weijing, HONG Leidong. Research progress of ultra-high temperature compost in the reduction of biological pollution such as antibiotic resistance genes in organic solid waste[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(12): 3061-3067.

图/表 1

参考文献 58

[1]	印遇龙, 杨哲. 天然植物替代饲用促生长抗生素的研究与展望[J]. 饲料工业, 2020, 41(24): 1-7.
[2]	刘玉芳. 四环素类抗生素在土壤中的迁移转化模拟研究[D]. 广州: 暨南大学, 2012.
[3]	江凌. 技术性贸易壁垒对我国农产品出口影响分析及应对策略研究[D]. 重庆: 西南大学, 2012.
[4]	SARMAH A K, MEYER M T, BOXALL A B A. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment[J]. Chemosphere, 2006, 65(5): 725-759.
[5]	吴娜娜, 钱虹, 李亚峰. 水中磺胺类抗生素去除技术研究进展[J]. 建筑与预算, 2017(6): 43-50.
[6]	杨凤霞, 毛大庆, 罗义, 等. 环境中抗生素抗性基因的水平传播扩散[J]. 应用生态学报, 2013, 24(10): 2993-3002.
[7]	王小彬, 闫湘, 李秀英. 畜禽粪污厌氧发酵沼液农用之环境安全风险[J]. 中国农业科学, 2021, 54(1): 110-139.
[8]	孙刚, 袁守军, 计峰, 等. 畜禽粪便中抗生素残留危害及其研究进展[J]. 环境与健康杂志, 2009, 26(3): 277-279.
[9]	孙刚. 畜禽粪便中四环素类抗生素检测分析及其在堆肥中的降解研究[D]. 合肥: 合肥工业大学, 2010.
[10]	钟为章, 陈赛男, 李月, 等. 好氧堆肥对抗生素抗性基因的消长影响研究进展[J]. 应用化工, 2022, 51(7): 2057-2063.
[11]	WHITEHEAD T R, COTTA M A. Stored swine manure and swine faeces as reservoirs of antibiotic resistance genes[J]. Letters in Applied Microbiology, 2013, 56(4): 264-267.
[12]	QIAN X, GU J, SUN W, et al. Diversity, abundance, and persistence of antibiotic resistance genes in various types of animal manure following industrial composting[J]. Journal of Hazardous Materials, 2018, 344: 716-722.
[13]	HALEY B J, KIM S W, SALAHEEN S, et al. Differences in the microbial community and resistome structures of feces from preweaned calves and lactating dairy cows in commercial dairy herds[J]. Foodborne Pathogens and Disease, 2020, 17(8): 494-503.
[14]	邹威, 金彩霞, 魏闪, 等. 华北地区不同规模畜禽养殖场粪便中抗生素抗性基因污染特征[J]. 农业环境科学学报, 2020, 39(11): 2640-2652.
[15]	CHENG W X, CHEN H, SU C, et al. Abundance and persistence of antibiotic resistance genes in livestock farms: a comprehensive investigation in Eastern China[J]. Environment International, 2013, 61: 1-7.
[16]	ZHANG Q Q, TIAN G M, JIN R C. The occurrence, maintenance, and proliferation of antibiotic resistance genes (ARGs) in the environment: influencing factors, mechanisms, and elimination strategies[J]. Applied Microbiology and Biotechnology, 2018, 102(19): 8261-8274.
[17]	QIAO M, YING G G, SINGER A C, et al. Review of antibiotic resistance in China and its environment[J]. Environment International, 2018, 110: 160-172.
[18]	徐永刚, 宇万太, 马强, 等. 环境中抗生素及其生态毒性效应研究进展[J]. 生态毒理学报, 2015, 10(3): 11-27.
[19]	CHEE-SANFORD J C, MACKIE R I, KOIKE S, et al. Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste[J]. Journal of Environmental Quality, 2009, 38(3): 1086-1108.
[20]	WANG Y Z, ZHANG Y L, LI J X, et al. Biogas energy generated from livestock manure in China: current situation and future trends[J]. Journal of Environmental Management, 2021, 297: 113324.
[21]	王森, 焦瑞峰, 马艳华, 等. 我国畜禽粪便综合利用途径研究[J]. 河南科技学院学报(自然科学版), 2017, 45(1): 20-24.
[22]	谷洁, 高华, 李鸣雷, 等. 施用有机无机复混肥对夏玉米生长和水分利用的影响[J]. 西北植物学报, 2004, 24(4): 638-642.
[23]	徐冰洁, 罗义, 周启星, 等. 抗生素抗性基因在环境中的来源、传播扩散及生态风险[J]. 环境化学, 2010, 29(2): 169-178.
[24]	汪涛, 杨再福, 陈勇航, 等. 地表水中磺胺类抗生素的生态风险评价[J]. 生态环境学报, 2016, 25(9): 1508-1514.
[25]	赵勇, 李欢, 张昭寰, 等. 食源性致病菌耐药机制研究进展[J]. 生物加工过程, 2018, 16(2): 1-10.
[26]	何敏艳. 高效铬还原菌Bacillus cereus SJ1和Lysinibacillus fusiformis ZC1的铬还原特性和全基因组序列分析[D]. 武汉: 华中农业大学, 2010.
[27]	钱昱良, 杨振边, 马保华, 等. 强力霉素降解菌在蛋鸡粪堆肥中的应用及其抗性风险研究[J]. 中国家禽, 2022, 44(10): 69-76.
[28]	张树清, 张夫道, 刘秀梅, 等. 高温堆肥对畜禽粪中抗生素降解和重金属钝化的作用[J]. 中国农业科学, 2006, 39(2): 337-343.
[29]	韦蓓, 黄福义, 苏建强. 堆肥对污泥中四环素类抗生素及抗性基因的影响[J]. 环境工程学报, 2014, 8(12): 5431-5438.
[30]	张丹, 彭双, 王丹青, 等. 鸡粪和猪粪生物发酵过程中抗生素抗性基因的动态变化[J]. 环境科学, 2023, 44(3): 1780-1791.
[31]	郑宁国, 黄南, 王卫卫, 等. 高温堆肥过程对猪粪来源抗生素抗性基因的影响[J]. 环境科学, 2016, 37(5): 1986-1992.
[32]	LIU Y W, FENG Y, CHENG D M, et al. Dynamics of bacterial composition and the fate of antibiotic resistance genes and mobile genetic elements during the co-composting with gentamicin fermentation residue and lovastatin fermentation residue[J]. Bioresource Technology, 2018, 261: 249-256.
[33]	LIAO H P, LU X M, RENSING C, et al. Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge[J]. Environmental Science & Technology, 2018, 52(1): 266-276.
[34]	YU Y S, CHEN L J, FANG Y, et al. High temperatures can effectively degrade residual tetracyclines in chicken manure through composting[J]. Journal of Hazardous Materials, 2019, 380: 120862.
[35]	韦蓓, 黄福义, 李虎, 等. 污泥堆肥过程中磺胺类和大环内酯类抗性基因的残留[J]. 应用与环境生物学报, 2014, 20(3): 395-400.
[36]	GOU C L, WANG Y Q, ZHANG X Q, et al. Effects of chlorotetracycline on antibiotic resistance genes and the bacterial community during cattle manure composting[J]. Bioresource Technology, 2021, 323: 124517.
[37]	MACGREGOR S T, MILLER F C, PSARIANOS K M, et al. Composting process control based on interaction between microbial heat output and temperature[J]. Applied and Environmental Microbiology, 1981, 41(6): 1321-1330.
[38]	XIAO Y, ZENG G M, YANG Z H, et al. Continuous thermophilic composting (CTC) for rapid biodegradation and maturation of organic municipal solid waste[J]. Bioresource Technology, 2009, 100(20): 4807-4813.
[39]	余震, 周顺桂. 超高温好氧发酵技术:堆肥快速腐熟与污染控制机制[J]. 南京农业大学学报, 2020, 43(5): 781-789.
[40]	廖汉鹏, 陈志, 余震, 等. 有机固体废物超高温好氧发酵技术及其工程应用[J]. 福建农林大学学报(自然科学版), 2017, 46(4): 439-444.
[41]	YU Z, TANG J, LIAO H P, et al. The distinctive microbial community improves composting efficiency in a full-scale hyperthermophilic composting plant[J]. Bioresource Technology, 2018, 265: 146-154.
[42]	OSHIMA T, MORIYA T. A preliminary analysis of microbial and biochemical properties of high-temperature compost[J]. Annals of the New York Academy of Sciences, 2008, 1125: 338-344.
[43]	TASHIRO Y, TABATA H, ITAHARA A, et al. Unique hyper-thermal composting process in Kagoshima City forms distinct bacterial community structures[J]. Journal of Bioscience and Bioengineering, 2016, 122(5): 606-612.
[44]	薛兆骏, 周国亚, 俞肖峰, 等. 超高温自发热好氧堆肥工艺处理剩余污泥[J]. 中国环境科学, 2017, 37(9): 3399-3406.
[45]	王晓诚, 郭颖, 颜开红. 超高温自发热好氧堆肥工艺处理生活垃圾探究[J]. 环境工程, 2020, 38(10): 183-189.
[46]	LIAO H P, LIU C, AI C F, et al. Mesophilic and thermophilic viruses are associated with nutrient cycling during hyperthermophilic composting[J]. The ISME Journal, 2023, 17(6): 916-930.
[47]	QIAN X, SUN W, GU J, et al. Reducing antibiotic resistance genes, integrons, and pathogens in dairy manure by continuous thermophilic composting[J]. Bioresource Technology, 2016, 220: 425-432.
[48]	XIE W Y, SHEN Q, ZHAO F J. Antibiotics and antibiotic resistance from animal manures to soil: a review[J]. European Journal of Soil Science, 2018, 69(1): 181-195.
[49]	GUO A Y, GU J, WANG X J, et al. Effects of superabsorbent polymers on the abundances of antibiotic resistance genes, mobile genetic elements, and the bacterial community during swine manure composting[J]. Bioresource Technology, 2017, 244(Pt 1): 658-663.
[50]	CHEN Z Q, WANG Y, WEN Q X. Effects of chlortetracycline on the fate of multi-antibiotic resistance genes and the microbial community during swine manure composting[J]. Environmental Pollution, 2018, 237: 977-987.
[51]	SELVAM A, XU D L, ZHAO Z Y, et al. Fate of tetracycline, sulfonamide and fluoroquinolone resistance genes and the changes in bacterial diversity during composting of swine manure[J]. Bioresource Technology, 2012, 126: 383-390.
[52]	LIAO H P, ZHAO Q, CUI P, et al. Efficient reduction of antibiotic residues and associated resistance genes in tylosin antibiotic fermentation waste using hyperthermophilic composting[J]. Environment International, 2019, 133(Pt B): 105203.
[53]	STALEY Z R, TUAN C Y, ESKRIDGE K M, et al. Using the heat generated from electrically conductive concrete slabs to reduce antibiotic resistance in beef cattle manure[J]. The Science of the Total Environment, 2021, 768: 144220.
[54]	KARADAG D, ÖZKAYA B, ÖLMEZ E, et al. Profiling of bacterial community in a full-scale aerobic composting plant[J]. International Biodeterioration & Biodegradation, 2013, 77: 85-90.
[55]	YAMADA T, SUZUKI A, UEDA H, et al. Successions of bacterial community in composting cow dung wastes with or without hyperthermophilic pre-treatment[J]. Applied Microbiology and Biotechnology, 2008, 81(4): 771-781.
[56]	张羽. 污水厂剩余污泥好氧堆肥控制因素及微生物特性的研究[D]. 长春: 吉林建筑大学, 2015.
[57]	MA W J, WANG L, XU X Y, et al. Fate and exposure risk of florfenicol, thiamphenicol and antibiotic resistance genes during composting of swine manure[J]. The Science of the Total Environment, 2022, 839: 156243.
[58]	GURMESSA B, PEDRETTI E F, COCCO S, et al. Manure anaerobic digestion effects and the role of pre- and post-treatments on veterinary antibiotics and antibiotic resistance genes removal efficiency[J]. The Science of the Total Environment, 2020, 721: 137532.